Pharmacodynamics of Siwu Decoction on blood supply based on factor analysis / 中国中药杂志

To evaluate the pharmacodynamic effect of Siwu Decoction in treating blood deficiency in mice under multidimensional pharmacodynamic indexes by factor analysis. The mouse blood deficiency model was established with cyclophosphamide combined with acetophenone; and mouse organ index,white blood cells,red blood cell,hemoglobin,platelet counts in whole blood,serum granulocyte-macrophage colony-stimulating factor,macrophagecolony-stimulating factor,promotion erythropoietin,interleukin-3 and interleukin-6 were used as indicators to characterize the blood-enriching effect of Siwu Decoction; the pharmacodynamic effect of Siwu Decoction on blood deficiency model was evaluated comprehensively by factor analysis. Four common factors were extracted from 14 pharmacodynamics indexes through the factor analysis,namely blood phase factor,viscera index,hematopoietic regulatory factor 1-spleen index and hematopoietic regulatory factor 2-viscera index. The cumulative contribution rate of variance reached 86. 581%. The comprehensive score of factor analysis showed that Siwu Decoction had the best effect on blood replenishment,and it is significant compared with the model group( P<0. 01). The effect of alcohol precipitation of Siwu Decoction was slightly decreased. The study showed that Siwu Decoction has the best blood-enriching effect,followed by water decoction and traditional decoction. Alcohol precipitation had the worst effect. Factor analysis can be used for the comprehensive evaluation of blood deficiency mice model,and is a suitable evaluation method for animal model for multi-dimensional multistage complex data analysis. It provides a new model to evaluate the efficacy of multidimensional data in the future.

Comparison on blood tonic effects of Jiaoai Decoction and Siwu Decoction on blood deficiency model rats / 中草药

Objective: To compare the effects of Jiaoai Decoction and Siwu Decoction on peripheral hemogram, immune organ, and energy metabolism enzyme activity in blood deficiency model rats, and investigate the mechanism of Jiaoai Decoction derived as a tonic effect of Siwu Decoction after the change. Methods: Taking every day on the fundus venous plexus put blood 5 mL/kg, 12 d continuous bleeding, to establish the rat model of blood deficiency, the red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), platelet (PLT), immune organ index, and energy metabolism enzyme activity were detected. Results: Compared with the normal control group, RBC, HGB, HCT, and PLT could be significantly decreased (P2+-Mg2+-ATP and Na+-K+-ATP enzyme activity could be decreased significantly (P2+-Mg2+-ATP and Na+-K+-ATP enzyme activity could be enhanced (P2+-Mg2+-ATP enzyme activity could be enhanced (P+-K+-ATP enzyme activity was significantly weaker than that in the mid-dose group of Jiaoai Decoction. Conclusion: Jiaoai Decoction and Siwu Decoction have the effect of enriching the blood in different degrees, Jiaoai Decoction evolved into Siwu Decoction could decrease the efficacy of enriching the blood.