ABSTRACT
Objective To investigate the effect of miR-107 on the function of human non-small cell lung cancer cell line A549 and its possible target genes.Methods The experiment was divided into the liposome+miR-107 mimics overexpression group(OV-miR-107 group),liposome+miR-107 inhibiting mimics downregulation group(KD-miR-107 group)and liposome+ negative control mimics group(NC group).The cell transfections of siRNA were siRNA-1,siRNA-2 and siRNA-3 respectively.The cellular prolifer-ation capacity was detected by MTT assay.The cellular cycle was detected by flow cytometry.The dual luciferase reporter gene as-say was performed to detect the downstream target gene of miR-107.The real time quantitative reverse transcription PCR and Western blot were respectively employed to examine mRNA and protein expression levels of downstream target gene.Results miR-107 inhibited the proliferation of A549 cells in a time-and dose-dependent manner(P<0.05).miR-107 arrested more A549 cells at the G0G1phase,and the proportions of S phase and G2M phase were decreased(P<0.05).miR-107 combinded with the 246 bp-253 bp of CCNE1 3′-untranslated region,and decreased mRNA and protein expression of CCNE1(P<0.05);after down-regulating CC-NE1 in A549 cells,siRNA-2 inhibited cellular proliferation(P<0.05)and blocked the cells to stay at G0G1phase(P<0.05).Con-clusion miR-107 inhibits the proliferation of human non-small cell lung cancer cell line A549 and regulates the cellular cycle by tar-geting CCNE1.
ABSTRACT
Objective To investigate the effect of elemene and the mechanism of this effect on the proliferation of neuroblastoma.Methods We treated cultured SK-N-SH cells with elemene and used the MTT assay to determine the inhibition of cell proliferation.We used RT-qPCR and Western blotting to measure the effect of elemene on mRNA and protein expression,respectively,of repressor element-1 silencing transcription factor (REST) in SK-N-SH cells and evaluated the mRNA expression of CCND1 and CCNE1 in SK-N-SH cells by using RT-qPCR.Results Elemene significantly inhibited the proliferation of SK-N-SH cells in a dose-and time-dependent manner (P =0.001 16).Moreover,the mRNA (P =0.000 38) and protein (P =0.003 39) expression of REST were markedly suppressed by elemene.Furthermore,elemene significantly reduced the mRNA expression of CCND1 (P =0.001 91) and CCNE1 (P =0.000 15),which are related to the cell cycle.Conclusion Elemene significantly suppressed the proliferation of neuroblastoma cells through the reduction of REST,CCND1,and CCNE1.