ABSTRACT
Objective: To investigate the relationship between rs4373814 polymorphisms of calcium channel (CACNB2) gene and the susceptibility of essential hypertension (EH) occurrence in Han population at northeastern area of China. Methods: A case-control study in Han population at northeastern of China was carried out and the research included 1998 participants as 2 groups: EH group,n=1006 patients treated in our hospital, and Control group,n=992 subjects with normal blood pressure from physical examination. The rs4373814 polymorphisms were examined by Snapshot technique and the statistical analysis was performed by SPSS 19.0 software. Results: The rs4373814 risk allele C frequency of CACNB2 gene was signiifcantly different between EH group and Control group,P=0.039, OR=1.068, 95% CI (1.004-1.137). Logistic regression analysis showed that with adjusted gender, age, BMI, smoking, drinking, in dominant model, the genotype rs4373814 carring CC + GC/GG obviously increased the risk of EH occurrence,P=0.037, OR=1.260, 95% CI (1.013-1.567). Conclusion: The rs4373814 polymorphisms of CACNB2 gene are related to the susceptibility of EH occurrence in Han population at northeastern area of China.
ABSTRACT
BACKGROUND: Vascular smooth muscle cells (VSMCs) proliferation is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent calcium channel. OBJECTIVES: To investigate the role of cytosolic Ca(2+) in the proliferation of VSMCs. METHODS: The proliferation of aortic vascular smooth muscle cells of rats(1% cholesterol diet fed rats and general diet fed ones), acquired by enzymatic method. Cell counting, and calcium agonists(Bay K 8644 10(-6)M) or calcium antagonists(verapamil 10(-6)M). Cytosolic calcium ion was measured with Fura 2 method. Calcium channel gene expresssion was detected with RT-PCR method. RESULTS: Masson Trichrome staining shows more disturbed cell lining, more VSMCs, more degenerated media, more collagen laydown, and more adventitial fat in the aorta of cholesterol-fed rats than in that of general diet-fed ones. VSMCs of cholesterol fed-rats were moreproliferated than those of general diet fed-ones. Bay K 8644 enhanced VSMCs proliferation of both groups. Verapamil blocked the incremental effects induced by Bay K 8644. Expression of calcium channel gene was enhanced by Bay K 8644 and was reversed by verapamil. CONCLUSION: The enhanced proliferation of atherosclerotic VSMCs is associated with the increment in cytosolic calcium, which is accomplshed through the expression of L-type voltage dependent calcium channel.