ABSTRACT
Objective@#To test the expression of miR-1178 in pancreatic cancer and study its clinicopathological significance and mechanism.@*Methods@#The expression of miR-1178 in 87 paired paraffin pancreatic ductal adenocarcinoma specimens and adjacent non- cancerous pancreatic tissue diagnosed by Pathology Department of Peking Union Medical College Hospital was detected by hybridization in situ. The relationship between the expression of miR-1178 and clinicopathological characters was analyzed.miR-1178 mimics and inhibitor were used to further detect the close relationship among miR-1178 and cancer invasion. Establish a nude mice subcutaneously transplanted tumor model, 4 weeks after vaccination for tumor volume and weight measurement.Student t-test, rank sum test, and χ2 test was used respectively to compare the data between two groups. Cox regression was adopted to improve the single factor and multiple factors analysis.@*Results@#The results of hybridization in situ showed the expression of miR-1178 was increased in 72 cases with pancreatic cancer compared to that in paired normal pancreatic tissues (50/72 vs. 11/72, χ2=43.26, P<0.05). miR-1178 expression was positively associated with tumor lymph node stage (χ2=4.189, P=0.041). Univariate and multivariate analysis revealed that miR-1178 was an independent adverse prognostic indicator for patients with pancreatic cancer (HR=2.364, 95%CI: 1.114-5.019, P=0.025). Transwell assay indicated the over-expression of miR-1178 increased the number of AsPC-1 cells that penetrated the ECM-coated membrane (177.0±19.8 vs. 119.7±15.9)(χ2=8.21, P<0.05). For the in vivo experiment, overexpression of miR-1178 significantly promoted tumor growth, compared with control group (tumor volume: (5 122.4±760.2)mm3 vs. (1 976.8±601.8)mm3, t=2.413, P<0.05; tumor weight: (1.55±0.21)g vs. (0.67±0.17)g, t=2.960, P<0.05). Over-expression of miR-1178 down-regulated the expression of Stub1 and elevated the expression of FAK/MMP-9 signal pathway(P<0.05).@*Conclusions@#MiR-1178 is overexpressed in pancreatic cancer, and is effective for predicting patients′ prognosis. MiR-1178 regulate Stub1/FAK/MMP-9 signal pathway and promote the invasion of AsPC-1 cells.
ABSTRACT
PURPOSE To investigate the relationship between the changes of extracellular matrix invaded by colorectal carcinoma and vascular endothelial cells,which will be useful to understand colorectal carcinoma biological behavior and may have clinical significance in the treatment of colorectal carcinoma.METHODS Immunohistochemistry technique and semi quantitative analysis were used.RESULTS As to the changes of ECM and endothelial cells,there were significant differences among the paracarcinoma groups,different invasive groups and normal group.THe increase of LN,CD34,F8 and actin were correlated with colorectal carcinoma invasion( P