ABSTRACT
The objective of this study is to improve the cephalosporin C production through optimization of medium and culture conditions .The effect of agitation speed on CPC production in 3 l bioreactor was investigated with fed batch mode. The maximum CPC (0.79 g/l) , the biomass ( 35.4g was obtained at 400 rpm A statistical method was introduced to optimized the main culture medium constituents. Some medium constituents involving glucose, ammonium sulphate as inorganic nitrogen source and methionine were found to be the most effective factors for (CPC) production. The results showed the priority of fed batch culture than the batch one, since it increase the yield of the CPC by about 155 %.
ABSTRACT
Acremonium chrysogenum NCIM 1069 was used for the biosynthesis of cephalosporin-C (CPC) in batch mode of cultivation. The effect of different medium constituents for better yield of CPC was thoroughly investigated. From the results of the fermentation, it was found that ammonium sulphate as inorganic nitrogen source and methionine at the concentration of 0.4 percent are most suitable for higher yield of antibiotic. The variation in the C/N ratio on the biosynthesis of CPC showed that a C/N ratio of 8.0 is most suitable for maximum production of CPC