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OBJECTIVE@#To observe the effect of bloodletting acupuncture at twelve -well points of hand on microcirculatory disturbance in mice with traumatic brain injury (TBI), and to explore the protective effect of bloodletting therapy on TBI.@*METHODS@#Sixty clean adult male C57BL/6J mice were randomly divided into a sham-operation group, a model group and a treatment group, 20 mice in each group. The TBI model was established by using electronic controlled cerebral cortex impact instrument in the model group and the treatment group. The mice in the treatment group were treated with bloodletting acupuncture at bilateral "Shaoshang" (LU 11), "Shangyang" (LI 1), "Zhongchong" (PC 9), "Guanchong" (TE 1), "Shaochong" (HT 9) and "Shaoze" (SI 1) immediately after trauma. The mice in the sham-operation group only opened the bone window but did not receive the strike. The regional cerebral blood flow (rCBF) was monitored by laser speckle contrast analysis (LASCA) using a PeriCam PSI System before trauma, immediately after trauma and 1, 2, 12, 24, 48, 72 h after trauma. The brain water content was measured by wet-dry weight method 24 h after trauma. The severity of functional impairment at 2, 12, 24, 48 and 72 h after trauma was evaluated by modified neurological scale scores (mNSS).@*RESULTS@#① 2 h after trauma, the mNSS in the model group and treatment group were >7 points, suggesting the successful establishment of model; compared with the sham-operation group, the mNSS was increased significantly from 12 to 72 h after trauma in the model group ( all <0.01), but the mNSS in the treatment group was significantly lower than that in the model group from 2 to 24 h after trauma (<0.01, <0.05). ② Compared with the sham-operation group, rCBF in the model group was decreased significantly immediately after trauma (<0.01), and the rCBF in the model group was lower than that in the sham-operation group from 1 to 72 h after trauma ( all <0.01); rCBF in the treatment group began to rise and was significantly higher than that in the model group 1-2 h after trauma (<0.01); 12-48 h after trauma, the increasing of rCBF in the two groups tended to be gentle until 72 h after injury, and rCBF in the model group was decreased while that in the treatment group continued to rise and was higher than that in the model group (<0.01). ③ 24 h after trauma, the brain water content in the model group was significantly higher than that in the sham-operation group (<0.01), and brain water content in the treatment group was significantly lower than that in the model group (<0.01).@*CONCLUSION@#The bloodletting acupuncture at twelve -well points of hand could improve microcirculation disturbance, increase microcirculation perfusion, alleviate secondary brain edema and promote the recovery of nerve function in mice with TBI.
Subject(s)
Animals , Male , Mice , Acupuncture Points , Acupuncture Therapy , Bloodletting , Brain Injuries, Traumatic , Therapeutics , Mice, Inbred C57BL , Microcirculation , Random AllocationABSTRACT
Background: Many clinical reports have indicated that ascorbic acid (vitamin C) improves vasodilatory impairments in patients with diabetes mellitus, but there is very little in vivo evidence to demonstrate its effectiveness on the brain. Objective: To investigate long-term effects of oral vitamin C administration on the cerebral microvascular vasodilation in diabetes, using streptozotocin (STZ)-induced diabetic rats. Materials and methods: Diabetes was induced in male Wistar Furth rats by a single intravenous injection of STZ (55 mg/kg b.w). Ascorbic acid (vitamin C) was administered in drinking water (1g/l). The rats were divided into control and diabetic groups with or without administration of vitamin C. The cerebral microcirculation was observed at different times (12, 24 and 36 weeks) after vitamin C supplementation, using fluorescence videomicroscopy. Responses of cerebral arterioles to acetylcholine (ACh), adenosine-5 diphosphate (ADP) and nitroglycerine (NTG) were studied by measuring diameters of cerebral arterioles before and after topical application on the cortical surface. Results: The vasodilatory responses of cerebral arterioles to ACh and ADP were significantly decreased in diabetic rats, compared with non-diabetic (control) rats. The response to NTG was not altered in diabetic rats, indicating that the vasodilatory impairment involves at the endothelium. The impaired endothelium-dependent vasodilation was prevented by long-term vitamin C administration. Conclusion: Long-term oral vitamin C administration might be of clinical relevance in improving cerebral microvascular vasodilatory impairment in diabetes.
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Objective:To observe DUXIL's curative effects and mechanisms for cerebral ischemia and anoxia secondary to the alteration of altering the number and configuration of human erythrocyte,which results in cerebral microcirculatory disturbance and dysunction of cerebrum.Methods:The patients were divided into two groups randomly and each group had 20 patients who had the prodrome of cerebral ischemia and anoxia.One group took DUXIL and the other NIMODIPINE for 15 days for comparison.Blood test and CVA were performed separately before and after the treatment.Twenty patients were chosed randomly from each group for SPECT.Results:There are varging degrees increases of parameters of erythrocyte acompanyed by alteration of parameters of CVA before treatment and SPECT qualitative analysis shows areas of low perfusion with differents severities.The number and configuration of erythrocyte was changed in group taken DUXIL.The parameters of CVA had been improved and the defect of encephalic perfusion also amended.The clinical symptoms were alleviated or disappeared.But no improvement were obtained in the other group.Conclusion:DUXIL can improve symptoms of cerebral ischemia and anoxia by affecting erythrocyte to make it have more favourable erythrocyte deformability(ED),which can contribute to effective perfusion of cerebral microcirculation.
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A new technique, the laser-doppler flowmetry. has been used intraoperatively to measure blood flow responses in the normal brain tissue, the traumatic brain tissue and so on. The flow estimate by this technique is based on the assessment of the doppler shift of the low power laser light, which is scattered by moving red blood cells.
Subject(s)
Brain , Craniotomy , Doppler Effect , Erythrocytes , Homeostasis , Laser-Doppler FlowmetryABSTRACT
Objective To observe the changes in cerebral microcirculation after subarachnoid hemorrhage(SAH) and to study its correlation with cerebral vasospasm(CVS).Methods Eighty-five SAH patients and 35 controls,enrolled in this study as perspective study subjects,were divided into SAH group and control group,CVS group and non-CVS group(nCVS),symptomatic CVS(sCVS) group and asymptomatic CVS(asCVS) group.All the subjects underwent CT perfusion(CTP) and the associated parameters,including cerebral blood flow(CBF),cerebral blood volume(CBV) and mean transit time(MTT),were recorded for final analysis.Results Compared to the control group,the CBF and MTT were significantly changed in SAH group(P