ABSTRACT
OBJECTIVE@#To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism.@*METHODS@#The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot.@*RESULTS@#MTT assay indicated bortezomib (2.5 μM, 5 μM, 10 μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48 h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9.@*CONCLUSIONS@#These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
ABSTRACT
Objective: To explore the effect of bortezomib on migration and invasion of cervical carcinoma HeLa cell and specific molecular mechanism. Methods: The effect of bortezomib on the viability of HeLa cell was measured by MTT assay. The effect of bortezomib on cell migration and invasion was measured by Transwell assay and invasion experiment respectively. The activation of Akt/mTOR signaling pathway and expression level of MMP2, MMP9 were assayed by western blot. Results: MTT assay indicated bortezomib (2.5μM, 5μM, 10μM) could inhibit HeLa cell viability, and the inhibitory rate was highest at 48h. Transwell assay and invasion experiment results showed that bortezomib inhibited HeLa cell migration and invasion. Western blotting assays presented bortezomib could suppress the phosphorylation of Akt and mTOR, and down-regulate the expression of MMP2 and MMP9. Conclusions: These results suggested bortezomib could inhibit migration and invasion in cervical carcinoma HeLa cell, which might be related to Akt/mTOR signal pathway.
ABSTRACT
OBJECTIVE: To prepare selened Lycium barbarum polysaccharides sulfates (Se-LBPS) and to investigate its antioxidation activity and inhibitory effect on Hela cell growth. METHODS: Se-LBPS was prepared through reaction of laboratory-made sulfated Lycium barbarum polysaccharides with sodium selenite using acedic acid as the catalyst, and it was characterized by means of Fourie transform infrared spectroscopy. The antioxidation activity was investigated by spectrophotometry, and the inhibitory action of Se-LBPS on the growth of human cervical carcinoma Hela cell in vitro was examined using MTT method. RESULTS: The eliminating ratios of hydroxyl radical and superoxide radical for Se-LBPS achieved 60.51% and 47.9%, respectively. The growth inhibiton ratio of human cervical carcinoma Hela cell by Se-LBPS was better than LBPS and selened Lycium barbarum polysaccharides (Se-LBP). The inhibition ratio was positively correlated with Se-LBPS concentration. An inhibition ratio of 59.67% was achieved with 200 μg · mL-1 Se-LBPS. CONCLUSION: Se-LBPS has obvious antioxidation activity which is stonger than selenium sweet potatoleaf polysaccarides, and marked inhibitory effects on human cervical carcinoma Hela cell growth stonger than folic acid. Copyright 2012 by the Chinese Pharmaceutical Association.