ABSTRACT
Objective: To investigate and analyze anticomplement and antitussive activities and the active ingredients of the extract of Chimonanthus nitens leaf. Methods: The classical anti-complement pathway and the concentrated ammonia-induced cough model was used to compare the activity of the different polar parts of C. nitens leaf, and the polar parts with anti-complement and antitussive activity were determined. A preliminary analysis of the chemical composition in activity extract was identified by high performance liquid chromatography-mass spectrometry. The main chemical components in the C. nitens leaf of antitussive and antibody activity were also evaluated. Results: The ethyl acetate extract of C. nitens leaf had both anti-complement and antitussive effects. A total of 28 compounds were initially identified through mass spectrometry analysis. Kaempferol-3-O-rutinoside and kaempferol had both antitussive and anti-complement activities. Conclusion: The ethyl acetate extract of C. nitens leaf has good anti-complement and antitussive activities, and the mainly active ingredients in it were kaempferol-3-O-rutinoside and kaempferol that could be used as quality-controlling chemical markers.
ABSTRACT
OBJECTIVE: To study the chemical constituents from the leaves of Jiangxi genuine medicinal material Chimonanthus nitens Oliv. METHODS: The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20, ODS column chromatography, semi-preparative HPLC, and so on. Their structures were elucidated on the basis of physiochemical properties and spectral data. RESULTS: Ten compounds were isolated and elucidated as uracil (1), 6, 7-dimethoxycoumarin (2), 6, 7, 8-trimethoxycoumarin (3), p-hydroxybenzoic acid ethyl ester (4), 1∶1 mixture of two diastereomers identical with (3RS, 6RS)-2, 6-dimethyl-octa-1, 7-dien-3, 6-diol (5 and 6), kaempferol (7), isofraxidin (8), scopoletin (9), and loliolide (10). CONCLUSION: Compounds 1, 3-6 and 10 are isolated from this plant for the first time and compounds 1, 4-6 are isolated from this genus for the first time.