ABSTRACT
Objective: To study the chemical constituents of Chinese eaglewood. Methods: The column chromatography on silica gel, Sephadex LH-20, and semi-preparative HPLC were used to separate and purify the compounds, their structures were elucidated by NMR and MS analyses. Results: Four compounds were isolated from the petroleum ether of Chinese eaglewood and identified as 6-methoxy-7-hydroxy-2-(2-phenylethyl) chromone (1), 6,7-dimethoxy-2-(2-phenylethyl) chromone (2), 6,7-dimethoxy-2-[2-(4'- methoxyphenyl) ethyl] chromone (3), and 6-methoxy-2-[2-(3'-methoxy-4'-hydroxyphenyl) ethyl] chromone (4). Conclusion: Compound 1 is a new compound named as Aquilarone J, and compound 4 is isolated from Chinese eaglewood for the first time.
ABSTRACT
OBJECTIVE: To establish the characteristic chromatogram of Chinese eaglewood by HPLC-DAD. METHODS: The analysis was performed on a Diamonsil C18 column(4.6 mm × 250 mm, 5 μm) with a gradient mobile phase of acetonitrile-0.1% formic acid at a flow rate of 0.7 mL · min-1. The detection wavelength was set at 252 nm and the column temperature was 30℃. Twenty-three batches of Chinese eaglewood samples were analyzed with the developed method, and similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine(2004A Version) was used for the quality assessment. RESULTS: Seventeen common peaks were found through the similarity evaluation system and 12 peaks were confirmed by comparison with the chromatograms of the standard substances. Among them, 9 peaks were identified as characteristic peaks of Chinese eaglewood. With peak 1 (agarotetrol) as the reference, the relative retention time of the other eight peaks were 1.033 6, 1.2193, 1.2486, 1.7833, 1.891 5, 1.9184, 2.404 6 and 2.4582, respectively. CONCLUSION: The developed method conforms to the requirements of methodology validation, and can be used for the quality control of Chinese eaglewood.
ABSTRACT
AIM@#To study the chemical constituents from the fermentation of the endophytic fungus HP-1 of Chinese eaglewood.@*METHODS@#The chemical constituents were isolated by column chromatography on silica gel and Sephadex LH-20, and their structures were elucidated on the basis of spectroscopic analysis.@*RESULTS@#Four compounds were isolated and identified as 3α, 3β, 10β-trimethyl-decahydroazuleno[6, 7]furan-8, 9, 14-triol (1), 4-hydroxyphenylacetic acid (2), 4-hydroxyphenethyl alcohol (3), and 5-hydroxymethyl-2-furancarboxaldehyde (4).@*CONCLUSION@#Compound 1 was a new compound. Compound 2 showed antibacterial activity against Staphylococcus aureus.