ABSTRACT
Objective To obtain the transcriptome dataset of Chloranthus japonicas. Methods Using the Illumina HiSeqTM 2000 150PE, a rhizome transcriptome of C. japonicus was generated, followed by systemic bioinformatics analyses. Results A total of 68 458 750 high quality clean reads were produced by the transcriptome sequencing. Trinity de novo assembling resulted in a total of 56 096 unigenes with an average length of 801 nt. BLAST analysis indicated that 25 773 (45.94%), 17 801 (31.73%), 16 082 (28.67%), and 9 649 (17.20%) unigenes were successfully annotated in the NR, Swiss-port, KOG, and KEGG databases, respectively. All unigenes were classified into three major groups by GO, including biological process, cellular component, and molecular function, and then, grouped into 40 subgroups. And 131 KEGG standard pathways were designated, 16 of which were defied as the secondary metabolism. Further analysis revealed that a total of 170 unigenes were involved in the biosynthesis of mono-, di-, sesqui-, or triterpene. Meantime, 1 887 unigenes were predicted to contain protein coding sequences. Totally 54 families of transcription factors of higher plant were identified. Using MISA prediction, 8 987 simple sequence repeats (SSRs) were obtained, among which the di-nucleotide SSRs were abundant with 5 948 (66.2%), whereas the penta-nucleotide SSRs were relatively less, accounting for 1.3%. Conclusion The transcriptome of C. japonicus rhizome was generated by RNA-seq along with the identification of unigenes implicated in various terpenes biosynthesis, which will provide a fundamental basis for secondary metabolism pathway dissections and their regulatory mechanisms in this plant species.
ABSTRACT
AIM To study the chemical constituents from Chloranthus japonicus Sieb..METHODS The ethyl acetate fraction of 95％ ethanol extract from C.japonicus was isolated and purified by silica,Sephadex LH-20,ODS and PHPLC column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were isolated and identified as p-hydroxyphenethyl (1),diisooctanephthalate (2),diisobutylphthalat (3),umbelliferone (4),ursolic acid (5),7α-hydroxysitosterol (6),chloranthalactone E (7),chloranthalactone B (8),apigenin (9),3-Sitosterol (10).CONCLUSION Compounds 1-3,6 are isolated from genus Chloranthus for the first time,compounds 4,5,9 are first isolated from this plant.
ABSTRACT
Objective:To optimize the extraction process of total flavonoids from Chloranthus japonicus sieb by Box-Behnken re-sponse surface methodology. Methods:The independent variables were the ethanol concentration ( X1 ) , liquid-solid ratio ( X2 ) and extraction time ( X3 ) , the dependent variable was the extraction amount of total flavonoids ( Y) , and the extraction process was opti-mized by Box-Behnken response surface methodology. Results: The results showed that the optimal extraction conditions for total fla-vonoids from Chloranthus japonicus sieb were as follows: the ethanol concentration was 54. 8%, the liquid-solid ratio was 13. 6, and the extraction time was 2. 0 h. The verified extraction process parameters of three batches showed that the relative error between the ex-perimental values and those predicted from the regression model was -2. 34%. Conclusion:The optimal extraction process parameters are simple and more convenient with higher precision for the extraction of total flavonoids from Chloranthus japonicus sieb.