ABSTRACT
Objective: To study the pharmacodynamics effect of Compound Jianshen Granules (CJG) on rats with type 2 diabetic nephopathy induced by high-fat and sugar diet combined low dose streptozotocin (STZ), and to investigate its mechanism. Methods: Type 2 diabetic nephropathy animal model was established by feeding with high fat and sugar diet combined with low dose of STZ. FBS, Scr, BUN, GHb, TC, TG, SOD, MDA, etc were measured to study its effects of reducing blood glucose and protecting kidney, data were dealed with SPSS17.0 software and analyzed in statistics, its pharmacodynamic effects were expounded by kidney and pancreas pathological section. MCP-1, ICAM-1, and IL-6 were tested by ELISA to probe into its mechanism tentatively. Results: Compared with the model group, the weight of each drug administration group of CJG entirely rised, and the difference had statistical significance (P < 0.01); The food, water, urine volume, and urine protein content of each drug administration group of CJG decreased, and the difference had statistical significance (P < 0.05, 0.01); The blood glucose level of each drug administration group of CJG decreased, and the difference had statistical significance (P < 0.05, 0.01); The Scr and BUN of each drug administration group of CJG decreased, and the difference had statistical significance (P < 0.05, 0.01); The GHb of each drug administration group of CJG decreased significantly (P < 0.05, 0.01); TC, TG, and MDA of each drug administration group of CJG decreased significantly (P < 0.01); SOD of each drug administration group of CJG increased significantly (P < 0.01); IL-6, MCP-1, and ICAM-1 of each drug administration group of CJG decreased significantly (P < 0.01). Conclusion: FBG and each biochemical criterion and pathological section results demonstrate that CJG have the certain effects of decreasing blood sugar and protecting kidney, moreover traeatment groups have significant difference compared with model groups. ELISA determination results indicate that the action mechanism of CJG is potentially related to MCP-1, ICAM-1, and IL-6 inflammatory pathway.
ABSTRACT
Objective: To optimize the extracting and processing conditions of Compound Jianshen Granules (CJG). Methods: UV analytical methods of total saponin and total polysaccharides were established, the optimal ethanol extraction process and water extraction process were confirmed according to single factor and orthogonal design test. The influence of different excipients and wetting agents were investigated, taking forming rate, solubility, and absorption rate as indexes, the materials added prescription varieties, ratio, and optimum molding process were screened. Results: The optimal ethanol extraction technology of CJG was: 85% ethanol, 10-fold, extracting for three times, 2 h once. The optimal water extraction technology was 14-fold, decocting 3 times, 30 min once. The optimized conditions of CJG were obtained with: accessories for the dextrin: lactose (4:1), main drug-accessories (1:1), with 85% ethanol as wetting agent which accounted for 15% of the main drug. Conclusion: The extraction process and forming process are feasible and stable, and the quality is controllable.