Glass Capping of Bacterial Culture Flasks

The use of cotton plug as closure of a bacterial culture flask had been reported to have many disadvantages such as inhibitory nature of cotton to certain microbes, chances of contamination during handling and accumulation of used cotton as biological waste. To overcome the disadvantages of cotton plugs, we have developed a new method of capping bacterial culture flasks. In the present study, three sets of experiments were conducted, one was to find out the efficiency of bacterial growth in culture flasks closed by either glass caps or cotton plugs and the second set was to find out the chances of getting contamination of sterile broth closed by either glass caps or cotton plugs and the third set was to find out the evaporation of water in conical flasks closed by glass caps or cotton plug. The results showed that the bacterial cultures closed by glass caps showed better growth with less chance of contamination and evaporation of the culture media. By this method, the bacterial culture work is made very simpler than using cotton plug.

Experiment of Detecting Total Dissolved Solids in Drinking Water Using Conical Flask Method / 环境与健康杂志

[Objective] To explor the possibility of application of conical flask as an evaperation vessel in detecting the content of total dissolved solids in drinking water. [Methods]The conical flask was placed on a electric stoveto dry, and in electric oven to bake until the weight of conical flask was constant, at temperature of 180±3℃, andthen was used to detect the total dissolved solids in drinking water. The comparison between conical flask methodand National Standard Method was made. [Results] The levels of total dissolved solids obtained according to conical-flask method and National Standard Method revealed no significaut differences (P＞0.5). [Conclusion] The detection of total dissolved solids in drinking water by conical flask method was rapid, accurate and practicabel.