Expression of cyclase-Associated protein 1 in breast cancer tissues and its clinical significance / 肿瘤

Objective: To investigate the expression of cyclase-Associated protein 1 (CAP1) in breast cancer tissues and its clinical significance. Methods: Overall 93 specimens of breast cancer tissues and the paracancerous tissues were collected. The expressions of CAP1 and Ki-67 in breast cancer tissues and the para-cancerous tissues were detected by immunohistochemistry. The correlations of CAP1 with clinicopathological features and the prognosis were analyzed. Results: The expression levels of CAP1 and Ki-67 in breast cancer tissues were higher than those in the para-cancerous tissues (both P 0.05). There was a positive correlation between CAP1 expression and Ki-67 in breast cancer tissues (r 2 = 0.403, P = 0.002). The differentiation, axillary lymph node status, CAP1 expression and Ki-67 were independent prognostic factors of breast cancer (all P < 0.05). Conclusion: The expression of CAP1 is high in breast cancer tissues. CAP1 may play an important role in proliferation in breast cancer cells.

The eukaryotic expression,intracellular location and functions of human CAP1 / 西安交通大学学报(医学版)

Objective To construct the recombinant eukaryotic expression plasmids of human adenylyl cyclase-associated protein 1 (CAP1)and to explore its intracellular location and functions.Methods By using Hela cDNA as the template,the cDNAs encoding CAP1 was amplified by PCR and inserted into pCMV-Myc vector to construct the recombinant plasmid.The recombinant plasmid was transfected into 293 cells using lipofectamine 2000.The protein expression and the intracellular location of the inserted gene were confirmed by Western blotting and immunofluorescence,respectively.Scratch-repair experiment was used to detect the cancer cells’ migration ability.Results The recombinant eukaryotic expression plasmid of human CAP1 was successfully constructed and transfected into eukaryote cells.The recombinant plasmid was successfully expressed in eukaryote cells.CAP1 was located in the cytoplasm.The results of scratch-repair experiment showed that the overexpression of CAP1 could significantly inhibit the cells’ migration.Conclusion CAP1 recombinant plasmid was successfully expressed in eukaryotic cells.CAP1 protein was located in the cytoplasm.The overexpression of CAP1 inhibited cell migration. The present study provides important experimental evidence for further study on CAP1.