ABSTRACT
OBJECTIVE:To investigate the decoction amount changes of verbascoside,gallic acid in single decoction and com-bined decoction of rehmanniae radix preparata,corni fructus and poria,and provide scientific basis for further study of effective substance basis. METHODS:The single decoction,combined decoction with each 2 medicine and combined decoction with the 3 medicines of rehmanniae radix preparata,corni fructus and poria,were respectively prepared. HPLC was adopted to detect and com-pare the decoction amount of active component verbascoside in rehmanniae radix preparata and active component gallic acid in cor-ni fructus in each group. RESULTS:Compared with single decoction of rehmanniae radix preparata,the decoction amount of ver-bascoside was decreased in combined decoction of poria+rehmanniae radix preparata(P<0.01),decoction amount of verbascoside was increased in combined decoction of corni fructus+rehmanniae radix preparata or combined decoction with the 3 medicines(P<0.01). Compared with single decoction of corni fructus,the decoction amount of gallic acid in each combined decoction was de-creased (P<0.01). CONCLUSIONS:The combined decoction of rehmanniae radix preparata,corni fructus and poria has certain promotion and inhibition effects on the decoction of verbascoside in rehmanniae radix preparata and inhibition effect on the decoc-tion of gallic acid in corni fructus. It is speculated verbascoside may be one of the main components in combined decoction playing the role of effectiveness.
ABSTRACT
OBJECTIVE:To study the effects of combined decoctions of alismatis rhizoma and curcumae radix and rehmanniae radix praeparata on the decoction amount of acteoside in rehmanniae radix praeparata,and provide reference for studying the effec-tive ingredients of three-drug effect. METHODS:Single decoction-1(rehmanniae radix praeparata 20 g),single decoction-2(alis-matis rhizoma 20 g),single decoction-3(curcumae radix 20 g),combined decoction-1(rehmanniae radix praeparata 20 g,alisma-tis rhizoma 20 g),combined decoction-2(rehmanniae radix praeparata 20 g,curcumae radix 20 g),combined decoction-3(alisma-tis rhizoma 20 g,curcumae radix 20 g)and combined decoction-4(rehmanniae radix praeparata 20 g,alismatis rhizoma 20 g,cur-cumae radix 20 g)were respectively taken to prepare dry extracts after extracting by refluxing,HPLC was used to detect the acteo-side content and calculate its decoction amount in sample. RESULTS:The decoction amounts of acteoside in single decoction-1, combined decoction-1,combined decoction-2 and combined decoction-4 dry extracts were 0.0354,0.0223,0.0228,0.0110 mg/g,respectively. Compared with single decoction-1 group,the last 3 groups had statistical significances(P<0.01);combined decoc-tion-4 showed lowest decoction amount in three-drug combined decoction group. Acteoside was not detected in the negative control groups(single decoction-2,single decoction-3 and combined decoction-3). CONCLUSIONS:The decoction amount of acteoside is reduced when alismatis rhizoma,curcumae radix and rehmanniae radix praeparata were decocted together,indicating that it may not be the main ingredient of playing effects in three-drug combined decoction liquid.
ABSTRACT
Objective: To develop an HPLC-DAD method for the detemination of the content change of honokiol and magnolol from classical Pingweisan (a prescription of Chinese materia medica used for stomach discomfort), and it is based on minister (Chen) medicine compatibility. Methods: After the decomposition of classical Pingweisan besed on Magnoliae Officinalis Cortex as minister medicine, 11 groups (one individual herb group, four compound groups, six groups with Magnoliae Officinalis Cortex as main medicine) were divided. The analysis was carried out on a GRACE AlltimaTM C18 (250 mm × 4.6 mm, 5 μm) column with a mobile phase which was composed of acetonitrile (B) and water (A) for isocratic elution. The column temperature was 25 ℃, the flow rate was 1 mL/min, and the detection wavelength was set at 294 nm. Results: The linear relationship of honokiol and magnolol is better within the ranges in 20.12-120.72 μg/mL (r = 0.999 0) and 20.16-120.96 μg/mL (r = 0.999 1); Study on the precision and repeatability, the RSD value of both honokiol and magnolol was less than 2%, which showed that the precision of the instrument and the method of repeatability were favourable. Stability test showed that the determination of the components was stable within 24 h. The recoveries were 98.69% and 101.28%, and the RSD values were 1.47% and 1.24%, which showed that the method had a good accuracy. Monarch Atractylodis Rhizoma, Zingberis, and Jujubae Fructus could make the contents of honokiol and magnolol in Pingweisan reduced; while the assistant Citri Reticulatae Pericarpium and guiding Glycyrrhizae Radix could make the contents increased. Conclusion: The other crude drugs in Pingweisan could affect and change the contents of honokiol and magnolol. It could explain the synergistic effect of compatibility and detoxification of the classical compound Pingweisan.
ABSTRACT
0.05).CONCLUSION:The small-packaged cut crude drugs wrapped with bag-steeping paper can replace the traditional ones.