The study of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve / 대한정형외과학회잡지

PURPOSE: To compare the effect of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve of rat. MATERIALS AND METHODS: Eighty Sprague-Dawley rats were divided into five experimental groups: orthotopic nerve graft as control group, inside-in vein graft with normal saline infiltration, inside-out vein graft with normal saline infiltration, inside-in vein graft with nerve growth factor infiltration and inside-out vein graft with nerve growth factor infiltration group. Animals of each group were sacrificed after electromyography at 2, 4, 8 and 12 weeks after operation. Also the light microscopy and transmission electron microscopy were performed to observe histologic change of grafted sites. RESULTS: This study demonstrates that autogenous vein graft serves as a conduit for nerve regeneration, and inside-out vein graft technique and nerve growth factor induce faster and more numerous axonal regeneration and earlier recovery of muscle power. CONCLUSION: Inside-out vein graft with nerve growth factor infiltration is supposed to be a promising technique to take the place of autogenous nerve graft.

The study of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve / 대한정형외과연구학회지

PURPOSE: To compare the effect of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve of rat. MATERIALS AND METHODS: Eighty Sprague-Dawley rats were divided into five experimental groups: orthotopic nerve graft as control group, inside-in vein graft with normal saline infiltration, inside-out vein graft with normal saline infiltration, inside-in vein graft with nerve growth factor infiltration and inside-out vein graft with nerve growth factor infiltration group. Animals of each group were sacrificed after electromyography at 2, 4, 8 and 12 weeks after operation. Also the light microscopy and transmission electron microscopy were performed to observe histologic change of grafted sites. RESULTS: This study demonstrates that autogenous vein graft serves as a conduit for nerve regeneration, and inside-out vein graft technique and nerve growth factor induce faster and more numerous axonal regeneration and earlier recovery of muscle power. CONCLUSION: Inside-out vein graft with nerve growth factor infiltration is supposed to be a promising technique to take the place of autogenous nerve graft.

The study of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve / 대한정형외과연구학회지

PURPOSE: To compare the effect of nerve regeneration with infiltration of normal saline and nerve growth factor after vein graft to the resected sciatic nerve of rat. MATERIALS AND METHODS: Eighty Sprague-Dawley rats were divided into five experimental groups: orthotopic nerve graft as control group, inside-in vein graft with normal saline infiltration, inside-out vein graft with normal saline infiltration, inside-in vein graft with nerve growth factor infiltration and inside-out vein graft with nerve growth factor infiltration group. Animals of each group were sacrificed after electromyography at 2, 4, 8 and 12 weeks after operation. Also the light microscopy and transmission electron microscopy were performed to observe histologic change of grafted sites. RESULTS: This study demonstrates that autogenous vein graft serves as a conduit for nerve regeneration, and inside-out vein graft technique and nerve growth factor induce faster and more numerous axonal regeneration and earlier recovery of muscle power. CONCLUSION: Inside-out vein graft with nerve growth factor infiltration is supposed to be a promising technique to take the place of autogenous nerve graft.

Experimental Study of Nerve Regeneration by Inside-in and Inside-out Vein Graft in the Nerve Defect of Rat / 대한정형외과학회잡지

PURPOSE: Autogenous vein graft as an alternative to autogenous nerve graft for bridging the gap of the peripheral nerve has been widely accepted. The purpose of this study was to find the comparative superiority between the two grafting techniques of inside-in and inside-out vein graft in rat model. MATERIALS AND METHODS: Thirty-three Sprague-Dawley rats were divided into three experimental groups: inside-in vein graft (group A), inside-out vein graft (group B) and control group. In group A, 15 mm jugular vein was grafted to a 10 mm gap created in the sciatic nerve. In group B, the same procedure was performed with group A, however, the vein was pulled through itself and its normal orientation was inverted before grafting. To compare the results of the two groups, electromyography and histologic studies were performed at 4, 8 and 12 weeks postoperative. RESULTS: In the electromyographic study, the mean of peak amplitude of compound motor action potential in group B was significantly higher than in group A at each experimental week. In the light and electron microscopic study, group B had more abundant regenerated axons and myelinized axonal fibers than group A at 4 weeks. At 8 and 12 weeks, there were no significant differences in the numbers and extents of myelinization of regenerated axons in both groups. CONCLUSION: This study demonstrates that inside-out vein grafting technique induces more faster axonal regeneration and earlier reinnervation to the denervated distal muscles in rat model.