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Objective: To study the chemical constituents from the flowers of Edgeworthia gardneri. Methods: The chemical constituents were isolated and purified by column chromatography on silica gel, AB-8 macroporous resin, sephadex LH-20, pre-HPLC. Their structures were determined on the basis of physicochemical properties and spectral analysis. Results: Four compounds were isolated from the flowers of E. gardneri and elucidated as gardnerol C (1), daphnoretin-5-O-β-D-glucopyranosyl- (1→2)-β-D-glucopyranoside (2), (3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (3), and dihydrokaempferol (4). Conclusion: Compound 1 is a new coumarin named gardnerol C, and compound 4 is obtained from the genus for the first time.
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Objective To study the chemical constituents from the leaves of Styrax japonicus. Methods Seventeen compounds including nine phenylpropanoids and seven other constituents were isolated from the chloroform fraction of the 70% ethanol extract of leaves of S. japonicus, using column chromatography over silica gel and Sephadex LH-20 as well as the method of recrystallization. Results On the basis of physical and chemical properties combined with spectral data analysis, their structures were elucidated as nectandrin B (1), stigmasterol (2), matteucinol (3), eupomatenoid-7 (4), β-stigmasterol (5), dehydrodiisoeugenol (6), 4-oxo- 4[(3β,22E)-stigmasta-5,22-dien-3-yloxy] butanoic acid (7), 4-(3-methory-4-hydroxy) pheny-3-methyl-3-buten-2-one (8), ursolic acid (9), vanillic acid (10), (+)-(7S,8R,8’R)-4,8’-dihydroxy-3-methoxy-1’,2’,3’,4’,5’,6’-hexanorligna-7,7’-lactone (11), (+)-(7S,8R)-4- hydroxy-3-methoxy-1’,2’,3’,4’,5’,6’,7’-heptanorlign 8’-one (12), (2S,3R’)-2,3-dihydro-2-(4-hydroxy-3-methoxyphenyl)-7-methoxy- 5-(2-propen-1-yl)-3-benzofuranmethanol (13), vanillin (14), (E)-p-coumaric acid (15), and dihydrokaempferol (16). Conclusion Compounds 1, 3-16 are isolated from this plant for the first time.
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Objective: To establish an HPLC digital fingerprint for flower petals of Danfeng (Paeonia ostii), and apply it in the determination of other six varieties of P. suffruticosa produced in Heze areas, while determining the contents of eight components in the flower petals. Methods: Nano-Micro C18 (250 mm × 4.6 mm, 5 μm) column was adopted and the gradient mobile phase consisted of acetonitrile (A)-0.1% formic acid (B) with a flow rate of 1.0 mL/min, the detective wavelength was 270 nm, and the column temperature was 25 ℃. Results: The chromatographic fingerprint similarity evaluation system for traditional Chinese medicines was used for analysis. The chromatographic fingerprint similarity of six batches of Danfeng samples was more than 0.993. There were altogether 14 common peaks, and eight of them were identified. A comparative analysis showed a similarity between samples of Danfeng and other six varieties produced in Heze. Meanwhile, the content of eight major chemical constituents, namely gallic acid, methyl gallate, kaempferol-3-O-β-D-glucopyranoside, apigenin-7-O-β-D-glucopyranoside, apigenin-7-O-β-D-neospheroside, dihydrokaempferol-7-O-β-D-glucopyranoside, paeoniflorin, and oxypaeoniflorin were determined in the range of 0.05-1.41, 1.01-4.41, 0.70-5.55, 4.60-18.30, 10.05-33.87, 0.09-1.76, 3.16-11.12, 0.85-2.02 mg/g. Conclusion: The combination of HPLC digital fingerprint and content determination could reflect its inherent quality in an all-round way, which provided scientific basis for the quality evaluation of P. suffruticosa.
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Objective: To investigate the chemical constituents from Ficus pumila and to provide the material basis for quality control. Methods: The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS, and preparative HPLC column chromatographies. Their structures were determined by physicochemical properties and spectral data analyses. Results: From 95% ethanol extract of F. pumila, 21 compounds were isolated and identified as rutin (1), kaempferol 3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside (2), isoquercitrin (3), quercitrin (4), dihydrokaempferol 5-O-β-D-glucopyranoside (5), dihydro-kaempferol 7-O-β-D-glucopyranoside (6), maesopsin 6-O-β-D-glucopyranoside (7), secoisolariciresinol 9-O-β-D-glucopyranoside (8), chlorogenic acid (9), protocatechuic acid (10), caffeic acid (11), 5-O-caffeoyl quinic acid methyl ester (12), p-hytroxybenzoic acid (13), vanillic acid (14), 5-O-caffeoyl quinic acid butyl ester (15), β-amyrin acetate (16), daucosterol (17), phaseic acid (18), vomifoliol (19), (1'S, 6'R)-8'-hydroxyabscisic acid β-D-glucoside (20), and 1-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid (21). Conclusion: Compounds 5-15, 18, 20, and 21 are isolated from this plant for the first time, and compound 5-8, 12, 15, 20, and 21 are found in plants of Ficus Linn. for the first time.
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OBJECTIVE: To investigate the chemical constituents of the leaves of Engelhardtia roxburghiana. METHODS: After the plant materials were extracted and fractionated with solvents, column chromatography was used to isolate and purify the compounds. The structures were elucidated on the basis of 1H-NMR, 13C-NMR, MS analysis and comparison with the literature data. RESULTS: Thirteen compounds were identified as following; taxilfolin (1), 5,7,4'-trihydroxy flavanone(2), kaempferol (3), dihydrokaempferol (4), quercetin(5), isoengeletin(6), engeletin (7), astilbin(8), isoastilbin(9), kaempferol-3-O-α-L-rhamnoside(10), eucryphin (11), 5-hydroxy-3,7,3',4'-tetramethoxyflavone(12), quercetin-3-O-α-L-rhamnoside (13). CONCLUSION: Compounds 2, 4, 9, 11 and 12 were identified from the title plant for the first time.