ABSTRACT
OBJECTIVE:To prepare Curcumine solid lipid nanoparticles(Cur-SLN).METHODS:Cur-SLN was prepared by emulsification evaporation-solidification at low temperature.The formulation of Cur-SLN was optimized by orthogonal experiment with the contents of curcumine,poloxamer 188,docosanoic acid glyceride lecithin used as factors and with entrapment efficiency as index;meanwhile,the appearance,particle size,surface potential,entrapment efficiency and the release profile of curcumine in vitro were studied.RESULTS:The optimized preparation conditions were as follows:the contents of curcumine,poloxamer,docosanoic acid glyceride lecithin were 8 mg,320 mg,140 mg and 320 mg,respectively.The Cur-SLN assumed spherical shape with particle size at(134?5)nm,entrapment efficiency at(67.4?1.3)% and mean surface potential at(-23.5?1.5)mV.The release profile of curcumine in vitro fitted Higuchi equation.CONCLUSION:It is feasible to prepare Cur-SLN by emulsion evaporation-solidification at low temperature,and the study results serve as experimental evidence for developing new curcumine injection.