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Objective To design and synthesize the conjugate (compound 1) of chlorin e6 (compound 3) with fluorouracil (5-Fu) as novel pH-responsive dual-mode antitumor photosensitizer by acyl hydrazone bond coupling, based on literature reports that combination of 5-Fu and photosensitizer possess synergistic anti-tumor effect, and investigate its photodynamic antitumor activity and mechanism. Methods Lead compound 3 was obtained by alkali degradation with 25% KOH-CH3OH on pheophorbide a (compound 4) which was prepared through acid hydrolysis of chlorophyll a in crude chlorophyll extracts from silkworm excrement. Reflux reaction of 5-Fu with P2S5 in pyridine formed crude 4-thio-5-fluorouracil which was followed to react with hydrazine hydrate (N2H4·H2O) in CH3OH to give 5-fluorouracil-4-hydrazone (compound 2). Then, treatment of compound 3 i.e. acid alkali degradation product of chlorophyll a in silkworm excrement with EDC·HCl generated its 171- and 152 cyclic anhydride which was followed to directly react with intermediate compound 2 to successfully get title compound 1. In addition, its pH-responsive 5-Fu release and photodynamic antitumor activity and their mechanisms in vitro were investigated. Results Compound 1 could responsively release 5-Fu at pH 5.0, with a cumulative release rate of 60.3% within 24 h. It exhibited much higher phototoxicity against melanoma B16-F10 and liver cancer HepG2 cells than talaporfin and its precursor compound 3, with IC50 value being 0.73 μmol/L for B16-F10 cells and 0.90 μmol/L for HepG2 cells, respectively. Upon light irradiation, it also could significantly induce cell apoptosis and intracellular ROS level and block cell cycle in S phase. Its structure was confirmed by UV, 1H-NMR, ESI-MS and elemental analysis data. Conclusion The conjugate compound 1 of compound 3 and 5-Fu has the advantages of strong PDT anticancer activity, high therapeutic index (i.e. dark toxicity/phototoxicity ratio) and responsively release 5-Fu at pH 5.0 etc. which shows “unimolecular” dual antitumor effects of PDT and chemotherapy and is worthy of further research and development.
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Professor FU Wen-bin, based on the concept of the "yang-oriented approach", identifies "yang deficiency with excess yin" as the underlying pathological mechanism of "five delays and five weaknesses" in children. He recommends a treatment model that included acupuncture, moxibustion, and rehabilitation, integrating various therapeutic methods of acupuncture, refined moxibustion, and auricular point sticking. Specific acupoints along the conception vessel, governor vessel, and bladder meridian, as well as related acupoints with tonifying effects on spleen and kidney, are selected to achieve the therapeutic goal of "promoting yang and nourishing yang simultaneously".
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Child , Humans , Acupuncture Therapy , Meridians , Moxibustion , Acupuncture Points , AcupunctureABSTRACT
Objective:To investigate the clinical efficacy of Yunkang oral liquid combined with dexamethasone and low-molecular-weight heparin in the treatment of recurrent spontaneous abortion (RSA) complicated with pre thrombotic state (PTS), and its effects on thromboelastogram and serum progesterone, β-human chorionic gonadotropin (β- hCG), and helper T cell (Th) 1/Th2 cytokine levels in patients with recurrent spontaneous abortion (RSA).Methods:A prospective selection of 94 RSA patients with PTS admitted to the Baoding Second Central Hospital from January 2015 to December 2020 was conducted. They were randomly divided into two groups using a random number table method, with 47 patients in each group. The control group was treated with dexamethasone combined with low molecular weight heparin, while the observation group was treated with Yunkang oral liquid in addition to the control group. Continuously treat until 12 weeks of pregnancy to evaluate the efficacy of both groups and observe the adverse reactions in both groups. Two sets of thromboelastogram parameters were compared before and after treatment, including clot formation time (K value), clot reaction time (R value), and clot formation rate (α angle), maximum clot strength (MA value), and coagulation comprehensive index (CI), as well as serum progesterone β- hCG, Th1/Th2 cytokine levels. The pregnancy outcomes of the two groups were followed up.Results:The total effective rate of the observation group [95.7%(45/47)] was significantly higher than that of the control group [83.0%(39/47), P<0.05]. After treatment, the K and R values of both groups were significantly prolonged compared to before treatment (all P<0.05), the angle α, MA value, and CI value were significantly reduced compared to those before treatment (all P<0.05); After treatment, the K and R values in the observation group were significantly longer than those in the control group (al P<0.05), The angleα, MA value, and CI value were significantly lower than those of the control group (all P<0.05). After treatment, the levels of β- hCG serum progesterone of the two groups were significantly higher than those before treatment (all P<0.05), and The β-hCG levels of the serum progesterone were all higher than those in the control group (all P<0.05). After treatment, The levels serum Th1 cytokines [interferon-γ (INF- γ), tumor necrosis factor- α (TNF- α)] of both groups were significantly decreased compared to those before treatment (all P<0.05), and the levels of serum Th2 cytokines [interleukin-4, IL-10] were significantly increased compared to those before treatment (all P<0.05); After treatment, the observation group's serum IFN- γ, TNF- α were significantly lower than those of the control group (all P<0.05), and The levels of serum IL-4 and IL-10 were significantly higher than those of the control group all P<0.05). The live birth rate of the observation group was 87.2%(41/47), which was higher than that of the control group [70.2%(33/47)] ( P<0.05). There was no statistically significant difference in the adverse reaction rate between the observation group [6.4%(3/47)] and the control group [4.3%(2/47)] ( P>0.05). Conclusions:The treatment of RSA combined with PTS with Yunkang oral liquid, dexmedroxyprogesterone, and low molecular weight heparin can significantly alleviate blood hypercoagulability, increase reproductive endocrine hormone levels, regulate Th1/Th2 cytokine balance, improve live birth rate and clinical efficacy, and is safe and reliable.
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Wumei Pill is a classic insect repellent prescription, which is used for ascaris lumbricoides and diarrhea. It is considered to be a representative prescription for the treatment of simultaneous occurrence of cold and heat syndromes. Its prescription is mainly composed of sour drugs ( Mume Fructus and bitter wine) and pungent drugs ( Aconiti Lateralis Radix Praeparataaconite, Cinnamomi Ramulus, Sichuan pepper, Angelicae Sinensis Radix, Zingiberis Rhizoma, and Asari Radix et Rhizoma), supplemented by bitter drugs ( Coptidis Rhizoma and Phellodendri Chinensis Cortex) and sweet drugs ( Ginseng Radix et Rhizoma and honey). From the point of view of "Tang-Ye-Jing-Fa Map", the role of liver is to disperse, whose deficiency leads to limb syncope, and excess leads to the full of hypochondrium and abdominal pain. The pungent herbs can tonify the liver-deficiency, the sour herbs can dispel the liver-excess, and sweet herbs can relive them both. The role of spleen is to moderate, whose deficiency leads to fatigue and weakness, and excess leads to the vomiting and diarrhea. The sweet herbs can tonify the spleen-deficiency, the pungent herbs can dispel the spleen-excess, and bitter herbs can relive them both. Therefore, the function of Wumei Pills is located in the liver and spleen, giving consideration to the heart and lung. It is used for the deficiency and excess mixed syndrome of liver and spleen, which is mainly characterized by chest and flank abdominal pain, limb chills, diarrhea, muscle and pulse contracture and the symptoms of the parts along the liver meridian. It is commonly used in the treatment of ascaris, diarrhea, impotence, depression. At the same time, it can also be used for the treatment of diseases about heart and lung, and cough, anxiety and other diseases.
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Objective:To explore the active components, targets and mechanism of Guizhi Fuling Pills in the treatment of atherosclerosis (AS) based on network pharmacology and molecular docking technology.Methods:The active components and potential target information of Guizhi Fuling Pills in the treatment of AS was obtained using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), SwissTargetPrediction database and Genecards database. The target protein interaction network was constructed by using STRING database. The DAVID database was used to perform the Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment on potential targets. AutoDockVina and PyMOL software were used to verify the molecular docking of the main active components and key targets of Guizhi Fuling Pills.Results:A total of 74 active components, 239 potential targets and 4 710 AS-related disease targets were screened, and 182 intersection targets were obtained. A total of 484 biological process items, 132 molecular function items and 74 cellular component items were obtained by GO functional enrichment analysis, and 116 signal pathways were screened by KEGG enrichment analysis. The results of molecular docking suggested that the active components of Guizhi Fuling Pills have good binding activity to the key intersection targets.Conclusion:The active components of Guizhi Fuling Pills, such as sitosterol and paeoniflorin, mainly treat AS by regulating estrogen signal pathway and inflammatory signal pathway through TNF, VEGFA and other targets.
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Objective:To observe the clinical efficacy of Mahuang Fuzi Xixin Decoction combined with balanced acupuncture in the treatment of migraine with yang deficiency and cold congealing syndrome.Methods:Randomized controlled trial. Totally 91 cases of migraine with yang deficiency and cold congealing syndrome in our hospital from March 2021 to May 2022 were selected and divided into 45 cases in the Western medicine group and 46 cases in the integrated Chinese and Western medicine group according to random number table method. The Western medicine group took flunarizine hydrochloride capsules orally, and the integrated Chinese and Western medicine group combined ephedrine and aconiti asarum decoction + balanced acupuncture treatment on the basis of the Western medicine group. Both groups were treated for 1 month. Traditional Chinese Medicine syndrome score was performed before and after treatment, the degree of headache was assessed by VAS scale, and the impact of headache on life was assessed by Headache Impact Test-6 (HIT-6); the levels of 5-hydroxytryptamine (5-HT) and calcitonin gene related peptide (CGRP) were determined by radioimmunoassay. The adverse reactions during treatment were observed and recorded, and the clinical efficacy was evaluated.Results:The total effective rate was 82.22% (37/45) in the Western medicine group and 95.65% (44/46) in the integrated Chinese and Western medicine group, with statistical significance ( χ2=4.20, P=0.041). After treatment, the scores of headache, hair, urine and cold limbs in the integrated Chinese and Western medicine group were lower than those in the Western medicine group ( t values were 18.49, 22.29, 21.31, 27.49, P<0.01), and the scores of VAS and HIT-6 were lower than those in the Western medicine group ( t values were 23.20, 9.33, P<0.01). After treatment, the level of 5-HT in the integrated Chinese and Western medicine group [(98.34±6.23) μg/L vs. (85.23±4.21) μg/L, t=11.74] was higher than that of the Western medicine group ( P<0.01); CGRP [(11.24±1.99) ng/L vs. (15.22±2.52) ng/L, t=8.37] was lower than that of the Western medicine group ( P<0.01). During treatment, the incidence of adverse reactions was 2.22% (1/45) in the Western medicine group and 2.17% (1/46) in the integrated Chinese and Western medicine group, without statistical significance ( P>0.05). Conclusion:Mahuang Fuzi Xixin Decoction combined with balanced acupuncture can effectively improve the clinical symptoms and life quality of patients of migraine with yang deficiency and cold congealing, and the clinical efficacy can be promoted.
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AIM:To assess the clinical efficacy of 5-fluorouracil(5-FU)and bandage contact lens in the pterygium excision combined with autogenous limbal stem cell transplantation(ALSCT)in treating patients with pterygium.METHODS:Random controlled clinical trial. A total of 71 patients(71 eyes)of pterygium who treated at the department of ophthalmology in Qinhuangdao Haigang Hospital between May 2021 and November 2022 were included. They were divide into three groups, including 23 eyes received pterygium excision combined with ALSCT in group A, 24 eyes that were administered with 5-FU intraoperatively and postoperatively in group B, and 24 eyes that received both bandage contact lens and 5-FU in group C. Furthermore, comfort levels at 1, 3, 7, 14d postoperatively, corneal epithelial healing at 1, 3, 7, 14d and 1mo postoperatively, treatment outcomes and complications at 3～6mo postoperatively were compared among the three groups of patients.RESULTS:The comfort levels at 1, 3 and 7d postoperatively and corneal healing at 1 and 3d postoperatively of the group C were better than those of the groups A and B. There were no statistical significant differences in the comfort levels at 14d after surgery and corneal healing at 14d and 1mo after surgery among the three groups of patients. Over a 3～6mo follow-up period, group A experienced recurrence in 3 eyes, group B had 1 recurrence, while group C had no recurrence. There were no statistically significant differences in complication rates among the three groups of patients.CONCLUSIONS: The application of 5-FU combined with bandage contact lens can enhance postoperative comfort levels, promote corneal epithelial healing, and improve the success rate in pterygium excision combined with ALSCT.
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We used network pharmacology to predict the mechanism in the treatment of rheumatoid arthritis (RA) via modified Gan Cao Fu Zi Decoction (GCFZ), and validated the results of the analysis and explored the pharmacodynamic effects of GCFZ through animal experiments. Firstly, TCMID, SymMap, HERB, STITCH and GEO databases were utilized to obtain the target genes of GCFZ for the treatment of RA, which yielded a total of 1 250 differentially expressed genes for RA, 534 genes for GCFZ targets and 83 intersecting genes. Then functional enrichment analysis of the intersecting genes was performed through GO and KEGG databases, and the results revealed that GCFZ and its active ingredients mainly functioned through cytokine pathways, where chemokine signaling pathway and tumor necrosis factor (TNF) signaling pathway were enriched with a high number of genes. Cytoscape 3.8.0 software was used to construct the drug-target-disease network and screen key proteins, which included TNF, C-X-C chemokine ligand 8 (CXCL8), C-X-C chemokine ligand 10 (CXCL10), C-C chemokine ligand 5 (CCL5), C-X-C chemokine ligand 2 (CXCL2) and C-X-C chemokine receptor type 4 (CXCR4). The molecular docking technology was used to confirm the binding ability of the main active ingredients of GCFZ to the core proteins. Additionally, the therapeutic effects of GCFZ in low (4 g·kg-1), medium (8 g·kg-1) and high (16 g·kg-1) dose groups were investigated by constructing the collagen-induced arthritis (CIA) rat model. X-ray imaging approach, HE staining and Safranin O-Fast Green staining showed that GCFZ treatment significantly improved bone destruction, synovial hyperplasia and cartilage damage in CIA rats, while immunofluorescence results showed that GCFZ treatment could regulate the expression of TNF, CXCL8 and CCL5. In summary, our results indicate that GCFZ contains a variety of small molecule pharmacodynamic substances, which can exert therapeutic effects via multiple targets and pathways, and obviously reduce the symptoms of arthritis in CIA rats. This animal experiment of our research was approved by the Experimental Animal Management and Ethics Committee of Bengbu Medical College.
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Developing analytical methods for the chemical components of natural medicines remains a challenge due to its diversity and complexity. Miao-Fu-Zhi-Tong (MFZT) granules, an ethnic Yi herbal prescription, comprises 10 herbs and has been clinically applied for gouty arthritis (GA) therapy. Herein, a series of chemical profiling strategies including in-house library matching, molecular networking and MS/MS fragmentation behavior validation based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) were developed for qualitative analysis of MFZT granules. A total of 207 compounds were identified or characterized in which several rare guanidines were discovered and profiled into alkyl substituted or cyclic subtypes. Moreover, network pharmacology analysis indicated that MFZT's anti-gout mechanism was mostly associated with the nuclear factor kappa-B (NF-κB) signaling, nucleotide oligomerization domain (NOD)-like signaling and rheumatoid arthritis pathways, along with the synergistic effect of 84 potential active compounds. In addition, a quantitative analytical method was developed to simultaneously determine the 29 potential effective components. Among them, berberine, pellodendrine, 3-feruloylquinic acid, neoastilbin, isoacteoside and chlorogenic acid derivatives at higher concentrations were considered as the chemical markers for quality control. These findings provide a holistic chemical basis for MFZT granules and will support the development of effective analytical methods for the herbal formulas of natural medicines.
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Humans , Chromatography, High Pressure Liquid/methods , Tandem Mass Spectrometry/methods , Drugs, Chinese Herbal/chemistry , Quality Control , Arthritis, GoutyABSTRACT
Aim To investigate the mechanism of action of Shen-Fu decoction in the prevention and treatment of cardiogenic shock based on network pharmacology and animal experiments. Methods The relevant targets and signaling pathways of cardiogenic shock of Shen-Fu decoction were predicted by network pharmacology, and a cardiogenic shock rat model was created by coronary artery ligation. Before modeling, rats were given the appropriate dose of Shen-Fu decoction or saline by gavage for 14 days according to the group, and real-time mean arterial pressure (MAP) changes were recorded after successful modeling. HE method was used to detect the myocardial histopathological changes of cardiogenic shock. TUNEL method was employed to detect rat myocardial cell apoptosis, and Western blotting was applied to determine the expression levels of rat myocardial Bax, Bcl-2, caspase-3, cleaved caspase-3 proteins. Results A total of 51 potential active ingredients of Shen-Fu decoction were screened out by network pharmacology, 80 targets of co-action with cardiogenic shock, and 43 core targets of close relationship between proteins, and GO enrichment analysis revealed that the core proteins were involved in the biology process (BP), mainly involving positive regulation of apoptotic process. KEGG enrichment analysis showed signaling pathways involving atherosclerosis-related, apoptosis and other signaling pathways. The results of animal model validation showed that Shen-Fu decoction could increase the shock blood pressure of rats with cardiogenic shock and alleviate the pathological changes of myocardial tissue, reduce the degree of apoptosis of rat cardiomyocytes, reduce the expression level of caspase-3, cleaved caspase-3 and Bax protein in rat myocardial tissue, and improve the expression level of Bcl-2 protein in myocardial tissue of rats. Conclusions The potential active ingredient of Shen-Fu decoction may play a role in the prevention and control of cardiogenic shock rats by acting on the target Bax, Bcl-2 to regulate the apoptosis signaling pathway of cardiomyocytes.
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Focusing on the phenomenon of "de-acupoints" of the needle insertion sites in Fu's subcutaneous needling (FSN), the authors allocated the evolution and characteristics of the needle insertion sites of FSN. From six aspects, named morphology and structure, location, nomenclature, numbers and meridian tropism, indications and acupuncture manipulations, the comparison was made between the insertion sites of FSN and traditional acupoints. It is believed: ①The needle insertion sites of FSN has the basic attributes of acupoint, which not only refers to the operation site, but also indicates the reaction of disease; moreover, it is the treatment site with significant therapeutic effect. ②The optimized sites of insertion in FSN should be named differently and their locations and numbers should be specified relatively. ③The insertion sites of FSN should be further intersected and integrated with traditional acupoints, and a part of traditional acupoints should become the insertion sites of FSN. ④Accepting and integrating the insertion sites of FSN, and expanding the scope of traditional acupoints may be the new project in the research of traditional acupoints.
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Moxibustion , Acupuncture Points , Acupuncture Therapy , Acupuncture , MeridiansABSTRACT
OBJECTIVE@#As a medicinal plant, the resource of Rhodiola dumulosa is deficient along with the large collection. For the protection and utilization of R. dumulosa, the influence of plant growth regulators (PGRs) on callus induction and adventitious shoots differentiation, polysaccharide production and the antioxidant activity were tested.@*METHODS@#Internodes of R. dumulosa were used as explants and cultured on MS medium plus different plant growth regulators (PGRs). The anti-oxidative activities of polysaccharides were evaluated using radical scavenging assays.@*RESULTS@#By response surface plot, 0.85 mg/L N6-benzyladenine (BA), 0.34 mg/L naphthaleneacetic acid (NAA) and 0.33 mg/L 2,4-dicholorophenoxyacetic acid (2,4-D) were the optimal factors for callus induction (90.03%) from internodes explants on MS medium. The fresh weight of green callus increased 47.26 fold, when callus was inoculated on MS + thidiazuron (TDZ) 0.5 mg/L + NAA 2.0 mg/L. Adventitious buds regenerated from callus on the media of MS were fortified with BA 1.0 mg/L plus NAA 0.5 mg/L, and the induction rate was 40.00%. MS plus indole-3-butyric acid (IBA) 1.0 mg/L produced the highest rooting rate with 10 to 15 roots in a length of 2-3 cm per shoot. The content of total polysaccharides in callus developed on MS + TDZ 0.5 mg/L + NAA 2.0 mg/L and MS + BA 1.0 mg/L + NAA 0.5 mg/L was as high as 1.72%-2.15%. At the dose of 0.5 mg/mL polysaccharides extracted from different callus induced on MS + NAA 2.0 mg/L + TDZ 0.5 mg/L or MS + BA 1.0 mg/L + NAA 0.5 mg/L or MS + BA 0.5 mg/L + 2,4-D 0.5 mg/L, the ABTS radical eliminating percentages were 82.78%, 80.18% and 68.59%, respectively, much higher than that of wild plant.@*CONCLUSION@#A rapid micropropagation system for R. dumulosa has been developed. The combination of TDZ and NAA or BA and NAA can increase the yield of the total polysaccharides. The polysaccharides isolated from callus and whole wild plants had stronger free radicals scavenging activities, indicating that polysaccharides from R. dumulosa are the potential pharmaceutical supplements.
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Poria(Fu Ling) is a bulk traditional Chinese medicine(TCM)with a long history and complex varieties. The royal medical records of the Qing Dynasty include multiple medicinal materials of Fu Ling, such as Bai Fu Ling(white Poria), Chi Fu Ling(rubra Poria), and Zhu Fu Ling(Poria processed with cinnabaris). The Palace Museum preserves 6 kinds of specimens including Fu Ling Ge(dried Poria), Bai Fu Ling, Chi Fu Ling, Zhu Fu Ling, Bai Fu Shen(white Poria cum Radix Pini), and Fu Shen Mu(Poria cum Radix Pini). After trait identification and textual research, we found that Fu Ling Ge was an intact sclerotium, which was processed into Fu Ling Pi(Poriae Cutis), Bai Fu Ling and other medicinal materials in the Palace. The Fu Ling in the Qing Dynasty Pa-lace was mainly from the tribute paid of the officials in Yunnan-Guizhou region. The tribute situation was stable in the whole Qing Dynasty, and changed in the late Qing Dynasty. The cultural relics of Fu Ling in the Qing Dynasty Palace confirm with the archival documents such as the royal medical records and herbal medicine books, providing precious historical materials for understanding Fu Ling in the Qing Dynasty and a basis for the restoration of the processing of the Fu Ling in the Qing Dynasty Palace.
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Animals , Poria , China , Books , Coleoptera , Medical Records , WolfiporiaABSTRACT
Huangdi Neijing (Yellow Emperor 's Canon of Medicine) establishes the theory that yuan-source points are indicated in zangfu diseases. However, compared with the yuan-source points of yin meridians for the treatment of zang-organ diseases, there is less attention to the indication of yuan-source points of yang meridians for fu-organ diseases, and this statement is even questioned. Collating the early literature and associating with the researches of medical experts, it is found that Nanjing (Classic of Difficult Questions) should be the theoretic origin that yuan-source points of yang meridians are indicated in fu-organ diseases. Regarding the reasons why this theory has not received clinical attention, the three aspects are involved, i.e. the theoretic completion of "he-sea points of three-foot-yang meridians for the diseases of six fu-organs", the limitation of the theory itself, and the lack of literature materials. It is proposed that the exploration on this theory should be deepened in views of the essence of yuan-source points relevant with the characteristic of the wrist-ankle pulse palpation region, acupoint combination, modern technology, etc.
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Meridians , Acupuncture Points , Foot , Lower Extremity , MedicineABSTRACT
In addition to the well-known thirteen ghost points in Qianjin Fang (Important Formulas worth a Thousand Gold Pieces), "XU Qiu-fu's thirteen ghost points" is recorded in other medical books. The contents of "XU Qiu-fu's thirteen ghost points" are not exactly same among these medical books, which mainly focus on the nomination and selection of the ghost points. Even though, there are many similarity when compared with the thirteen ghost points in Qianjin Fang in terms of acupoint selection, acupoint location and the sequence of needle insertion, as well as the therapeutic methods. The authors believe that "XU Qiu-fu's thirteen ghost points" is actually adapted based on the framework of the thirteen ghost points in Qianjin Fang.
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Acupuncture Points , Books , Gold , Needles , RecordsABSTRACT
This paper summarizes the status of application and research of Fu's subcutaneous needling for peripheral facial paralysis, and the characteristics of different stages of peripheral facial paralysis treated with Fu's subcutaneous needling are analyzed from the aspects of intervention timing, protocol design, needle insertion point, sweeping and reperfusion activity, tube retaining time and acupuncture frequency. It is found that there are no norms and standards in sweeping and reperfusion, tube retention and acupuncture frequency in clinical application，and the exploration of staged treatment is insufficient in the research. In the future, it is necessary to form standardized operation to promote clinical promotion, and improve the research on treatment rules and mechanism according to the characteristics of disease stage.
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Humans , Facial Paralysis/therapy , Vascular Surgical Procedures , Acupuncture Therapy , NeedlesABSTRACT
ABSTRACT Introduction Kung fu is a Chinese martial art with continuous movements based on step movement and embodied by the transformation of body shapes, requiring the athletes to strengthen the lower limbs. Joint and muscle analysis are critical to verify the strength of athletes' lower limbs and play a guiding role in evaluating the level of recovery for injured athletes. Objective Explore the myoarticular recovery effect after kung fu training. Methods 24 kung fu athletes were randomly divided into experimental and control groups, with 6 males and 6 females in each group. The athletes in the experimental group received traditional martial training for 60 minutes, while the control group received relaxation training simultaneously. The experiment lasted for 6 months and was performed 6 times a month. Results After kung fu training, the rates of the experimental group were higher than those of the control group. Conclusion Kung fu training positively impacts the athletes' muscle strength and joint strength. Kung fu training can also improve the level of myoarticular recovery and improve the flexibility of its practitioners. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução O kung fu, é uma arte marcial chinesa com movimentos contínuos baseados no movimento de passos e materializado pela transformação da forma corporal, requerendo dos atletas uma boa força dos membros inferiores. A análise das articulações e dos músculos são fundamentais para verificar a força dos membros inferiores dos atletas e desempenham um papel guia na avaliação do nível de recuperação aos atletas lesionados. Objetivo Explorar o efeito de recuperação mioarticular após o treinamento de kung fu. Métodos 24 atletas de kung fu foram divididos aleatoriamente em grupo experimental e controle, com 6 homens e 6 mulheres em cada grupo. Os atletas do grupo experimental receberam treinamento marcial tradicional durante 60 minutos, enquanto o grupo de controle recebeu treinamento de relaxamento durante o mesmo tempo. O experimento durou 6 meses e foi realizado 6 vezes por mês. Resultados Após o treinamento de kung fu, os índices do grupo experimental foram maiores que os do grupo de controle. Conclusão O treinamento de kung fu revelou impactos positivos sobre a força muscular e a força articular dos atletas. O treinamento de kung fu pode melhorar ainda o nível de recuperação mioarticular e aprimorar a flexibilidade de seus praticantes. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción El kung fu, es un arte marcial chino con movimientos continuos basados en el movimiento de pasos y materializados por la transformación de la forma corporal, requiriendo de los atletas una buena fuerza de los miembros inferiores. El análisis de las articulaciones y los músculos es fundamental para verificar la fuerza de los miembros inferiores de los deportistas y desempeña un papel orientador en la evaluación del nivel de recuperación de los deportistas lesionados. Objetivo Explorar el efecto de recuperación mioarticular después del entrenamiento de kung fu. Métodos 24 atletas de kung fu fueron divididos aleatoriamente en grupo experimental y de control, con 6 hombres y 6 mujeres en cada grupo. Los atletas del grupo experimental recibieron entrenamiento marcial tradicional durante 60 minutos, mientras que el grupo de control recibió entrenamiento de relajación durante el mismo tiempo. El experimento duró 6 meses y se realizó 6 veces al mes. Resultados Tras el entrenamiento de kung fu, los índices del grupo experimental fueron superiores a los del grupo de control. Conclusión El entrenamiento de kung fu reveló impactos positivos en la fuerza muscular y la fuerza articular de los atletas. El entrenamiento de kung fu también puede mejorar el nivel de recuperación mioarticular y aumentar la flexibilidad de sus practicantes. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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ABSTRACT Introduction: Kungfu is a traditional Chinese sports activity, and many optional courses are offered in many universities. However, during kung fu practice, sports injuries can be caused. Objective: Investigate the most common types of sports injuries caused by kung fu practice and present intervention countermeasures. Methods: A total of 612 college students practicing kung fu at 5 universities were investigated, and a 12-week follow-up survey was conducted to collect data on the types, causes, and injury grades of their sports injuries. According to the survey results, reasonable prevention and intervention measures were formulated to ensure the safety of kung fu practice. Results: The sports injuries caused by kung fu practice mainly occurred at the waist, knees, and ankles, and the types of sports injuries were mainly by ligament strain, which was mainly caused by improper preparation, poor physical functioning, and mental state, incorrect technical movements, etc. Conclusion: Kung fu practice can improve the physical health of college students, but it can also lead to sports injuries, especially to the pelvic girdle, knees, and ankles. Strict preventive measures should be taken to prevent sports injuries from affecting the physical health of college students. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução: O kung fu é uma atividade esportiva tradicional chinesa, sendo que muitos cursos opcionais são oferecidos em várias universidades. Porém, durante a prática de kung fu, lesões esportivas podem ser causadas. Objetivo: Investigar os tipos mais comuns de lesões esportivas causadas pela prática de kung fu e apresentar as contramedidas de intervenção. Métodos: Um total de 612 estudantes universitários praticantes de kung fu em 5 universidades foram investigados, uma pesquisa de acompanhamento de 12 semanas foi realizada para coletar dados sobre os tipos, causas e graus de lesão de suas lesões esportivas. De acordo com os resultados da pesquisa, foram formuladas medidas razoáveis de prevenção e intervenção para garantir a segurança da prática de kung fu. Resultados: As lesões esportivas causadas pela prática de kung fu ocorreram principalmente na cintura, joelhos e tornozelos, e os tipos de lesões esportivas foram principalmente por tensão ligamentar, que foi causada principalmente por preparação inadequada, mau funcionamento físico e estado mental, movimentos técnicos incorretos, etc. Conclusão: A prática de kung fu pode melhorar a saúde física dos estudantes universitários, mas também pode levar a lesões esportivas, principalmente na cintura pélvica, joelhos e tornozelos. Medidas preventivas rigorosas devem ser tomadas para evitar que lesões esportivas afetem a saúde física dos estudantes universitários. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción: El kung fu es una actividad deportiva tradicional china, y en muchas universidades se ofrecen cursos opcionales. Sin embargo, durante la práctica del kung fu pueden producirse lesiones deportivas. Objetivo: Investigar los tipos más comunes de lesiones deportivas causadas por la práctica del kung fu y presentar las contramedidas de intervención. Métodos: Se investigó a un total de 612 estudiantes universitarios que practicaban kung fu en 5 universidades, y se realizó una encuesta de seguimiento de 12 semanas para recoger datos sobre los tipos, las causas y los grados de lesión de sus lesiones deportivas. Según los resultados de la investigación, se formularon medidas razonables de prevención e intervención para garantizar la seguridad de la práctica del kung fu. Resultados: Las lesiones deportivas causadas por la práctica del kung fu se produjeron principalmente en la cintura, las rodillas y los tobillos, y los tipos de lesiones deportivas fueron sobre todo por distensión de los ligamentos, lo que fue causado principalmente por una preparación inadecuada, un mal funcionamiento físico y un estado mental, movimientos técnicos incorrectos, etc. Conclusión: La práctica del kung fu puede mejorar la salud física de los estudiantes universitarios, pero también puede provocar lesiones deportivas, principalmente en la cintura pélvica, las rodillas y los tobillos. Deben adoptarse estrictas medidas preventivas para evitar que las lesiones deportivas afecten a la salud física de los estudiantes universitarios. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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ABSTRACT Introduction Many studies have explored different training methods to improve cardiorespiratory capacity, ensuring better oxygen demand to help students develop better sports skills and teaching outcomes. Objective Explore the effects of applying resistance training on kung fu teaching. Methods 40 kung fu athletes were selected as research volunteers. They were randomly divided into experimental and control groups; both were trained in a cardiopulmonary resistance protocol for 8 weeks, three times a week, using a breathing trainer. The control group trained at 20%, while the experimental group had their training at 50% of full capacity. Results The maximal inspiratory pressure, FEV1 / FVC, and mvv15 of the lung function indices increased significantly in the experimental group; the forced capacity and maximal ventilation of the oxygen uptake capacity indices also increased significantly. The blood oxygen saturation and the blood flow perfusion index of the oxygen utilization capacity indices had significant differences between the groups; the central balance and flexibility indices also showed significant differences. Conclusion After cardiopulmonary endurance training, the athletes' capacity was significantly improved, inferring a benefit in the quality of kung fu teaching. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.
RESUMO Introdução Muitos estudos têm explorado explorando diferentes métodos de treinamento para melhorar a capacidade cardiorrespiratória, garantindo uma melhor demanda de oxigênio para ajudar os estudantes a desenvolverem melhores habilidades esportivas e resultados do ensino. Objetivo Explorar os efeitos da aplicação do treinamento de resistência sobre o ensino de kung fu. Métodos 40 atletas de kung fu foram selecionados como voluntários de pesquisa. Eles foram divididos aleatoriamente em grupo experimental e controle, ambos foram treinados num protocolo de resistência cardiopulmonar durante 8 semanas, três vezes por semana, utilizando um treinador respiratório. O grupo controle treinou a 20% enquanto o experimental teve seu treinamento a 50% de capacidade total. Resultados A pressão inspiratória máxima, VEF1 / FVC e mvv15 dos índices de função pulmonar aumentou significativamente no grupo experimental, a capacidade forçada e a ventilação máxima dos índices de capacidade de absorção de oxigênio também aumentaram significativamente. A saturação de oxigênio no sangue e o índice de perfusão do fluxo sanguíneo dos índices de capacidade de utilização de oxigênio tiveram diferenças significativas entre os grupos, o equilíbrio central e os índices de flexibilidade também evidenciaram diferenças significativas. Conclusão Após o treinamento de resistência cardiopulmonar, a capacidade dos atletas foi significativamente aprimorada, inferindo um benefício na qualidade do ensino de kung fu. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.
RESUMEN Introducción Muchos estudios han explorado diferentes métodos de entrenamiento para mejorar la capacidad cardiorrespiratoria, asegurando una mejor demanda de oxígeno para ayudar a los estudiantes a desarrollar mejores habilidades deportivas y resultados de enseñanza. Objetivo Explorar los efectos de la aplicación del entrenamiento de resistencia en la enseñanza del kung fu. Métodos Se seleccionaron 40 atletas de kung fu como voluntarios para la investigación. Se dividieron aleatoriamente en grupo experimental y grupo de control, ambos fueron entrenados en un protocolo de resistencia cardiopulmonar durante 8 semanas, tres veces por semana, utilizando un entrenador de respiración. El grupo de control entrenó al 20% mientras que el grupo experimental lo hizo al 50% de su capacidad. Resultados La presión inspiratoria máxima, VEF1 / FVC y mvv15 de los índices de función pulmonar aumentaron significativamente en el grupo experimental, la capacidad forzada y la ventilación máxima de los índices de capacidad de captación de oxígeno también aumentaron significativamente. La saturación de oxígeno en sangre y el índice de perfusión del flujo sanguíneo de los índices de capacidad de utilización de oxígeno presentaron diferencias significativas entre los grupos, los índices de equilibrio central y de flexibilidad también mostraron diferencias significativas. Conclusión Tras el entrenamiento de resistencia cardiopulmonar, la capacidad de los atletas mejoró significativamente, lo que infiere un beneficio en la calidad de la enseñanza del kung fu. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.
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[Objective] The Zang-Fu and the meridians, which are one of the foundations of modern acupuncture and moxibustion medical education, appeared in "Lingshu" in the "Meridian" section. However, the Zang-Fu and the meridians each has a history of independent development in ancient China Before the Common Era. The purpose of this study is to investigate the process by which the meridians came to be associated with the Zang-Fu, drawing from descriptions in "Suwen", which was compiled Before the Common Era.[Method] The source literature of this study is "Revisions and Extensive Notes on the Huangdi Neijing Suwen", with reference to "Acupuncture and Moxibustion Medicine and Classic Research" by MARUYAMA Masao to classify compilations of "Suwen". The research items are the names of the Zang and the Fu, the relationships between the Zang and the Fu, and the piao-li relationships of the Zang-Fu and the three Yin and three Yang of the hands and feet. [Result] The names for the Zang were Liver, Heart, Spleen, Lungs and Kidneys, and for the Fu were Small Intestine, Bladder, Gallbladder, Large Intestine, Stomach, and Sanjiao. The Zang-Fu relationships were Lungs-Large Intestine, Spleen-Stomach, Heart-Small Intestine, Kidney-Bladder, Liver-Gall Bladder, and Chronic Illness-Sanjiao. The piao-li relationships of the Zang-Fu and the three Yin and three Yang of the hands and feet were Foot Thai-Yang and Shao-Yin, Foot Shao-Yang and Chueh-Yin, Foot Yang-Ming and Thai-Yin, Hand Thai-Yang and Shao-Yin, Hand Yang-Ming and Thai-Yin, and Hand Shao-Yang and Heart. The Liver is treated by Foot Chueh-Yin and Shao-Yang, the Heart is treated Hand Shao-Yin and Thai-Yang, the Spleen is treated by Foot Thai-Yin and Yang-Ming, the Lungs are treated by Hand Thai-Yin and Yang-Ming, and the Kidneys are treated by Foot Shao-Ying and Thai-Yang. There was the expression "太陰陽明表裏為脾胃脈也" that Hand-Foot, Yin-Yang and the Zang-Fu were grouped by their Piao-li relationships. And there were the expressions saying "Shao-Yin is the Kidneys" and "Yang-Ming is the Stomach", although these were not in complete form. [Discussion] The Zang-Fu meridians, combining the Zang-Fu and the meridians, started from the Spleen-Stomach. There were other expressions combining the Zang-Fu with the meridians, although these were also not in complete form. While medicine at that time was being organized and integrated with the Yin-Yang and Five Elements theories, the relationships between the Zang-Fu and the meridians were also established. And it is inferred that the process of combining these and developing them into a new medical system existed during this period. [Conclusion] The relationships between the Zang-Fu and the meridians were established in "Suwen", which was compiled Before the Common Era, and the expression combining them started from "太陰陽明表裏為脾胃脈也".