ABSTRACT
OBJECTIVE: To develop an HPLC method for simultaneous determination of seven components in traditional Chinese medicines Fangfengtongsheng pills from different factories. METHODS: The chromatographic analyses were carried out on a kromasil C18 column(4.6 mm × 250 mm, 5 μm); and the mobile phase was methanol(A)-0.5 acetic acid at 1.0 mL · min-1 in gradient elution mode which was 15% A from 0 to 10 min, 15%-30% A from 10 to 15 min, 30%-40% A from 15 to 40 min, 40%-70% A from 40 to 80 min, and 70%-90% A from 80-120 min; the column temperature and detection wavelength were set at 25°C and 280 nm, respectively. RESULTS: Seven components were determined by HPLC, including baicalin, wogonoside, baicalein, wogonin, chrysin, emodin, and chrysophanol. The recoveries were 101.3%, 98.6%, 99.7%, 96.4%, 102.7%, 100.2% and 98.4%, respectively, and the linearity were good (r ≥ 0.9992) in the concentration ranges of 2.33-300.0, 0.407-120.0, 1.563-50.0, 0.625-40.0, 0.625-77.8, 1.563-100, and 1.625-120 μg · mL-1. CONCLUSION: Seven components are analyzed in Fangfengtongsheng pills by RP-HPLC with gradient elution, and the method has good specificity, reproducibility and high sensitivity, which can be applied to control the quality of Fangfengtongsheng pills.
ABSTRACT
Objective: To investigate the application of microemulsion TLC in separation and identification of various effective compositions in traditional Chinese patent medicines. Methods: The various compositions in Kangganmao Granules and Fangfeng Tongsheng Pills were separated and identified simultaneously on polyamide film with six kinds of SDS/n C 4H 9OH/n C 7H 16 /H 2O microemulsions as the mobile phases. Affections of kinds and acidity on separationg results of microemulsions were investigated. Results: The baicalin, chlorogenic acid, Saposhnikovia and glycyrrhiza in Kangganmao Gronules and the emodin, chrysophanol, baicalin, Saposhnikovia and Glycyrrhizae in Fangfeng Tongsheng Pills may be separated and identified simultaneously with microemulsion (containing 75% water) formic acid (9∶1) as mobile phase. Conclusion: Microemulsion TLC is an efficient, simple new method for the separation and identification of various compositions in traditional Chinese patent medicines.