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Purpose To examine the expression of Fascin-1 and β-catenin protein and K-ras gene mutation in colorectal adenocarcinoma, and to explore their role in progression of colorectal neoplasm and their relevance. Methods Fascin-1 and β-catenin were analyzed by use of immunohistochemistry En Vision two-step. K-ras gene mutation was detected by ARMS method.Relationship between overexpression of Fascin-1, the nuclear expression of β-catenin, and the mutations of K-ras gene and clinicopathologic parameters was analyzed, the correlation between them was also analyzed. Results In 112 colorectal adenocarcinoma samples, the overexpression rate of Fascin-1 protein was 27.7％ (31/112), significantly higher than non-neoplastic mucosa (P < 0.01). The high nuclear expression rate of β-catenin was 29.5％ (33/112) in adenocarcinoma and non-neoplastic mucosa respectively with a significant difference between two groups (P < 0.01). High expression rate of Fascin-1 protein and β-catenin were correlated significantly with lymph node metastasis (P = 0.022, P = 0.027), and TNM staging (P =0.042, P = 0.019) in colorectal adenocarcinoma. The overexpression of Fascin-1 protein was correlated with tumor location (P = 0.004). The mutation rate of K-ras gene was 34.8％ (39/112), which showed no correlation with age, gender, tumor size, grade of differentiation, lymph node metastasis and TNM staging (P> 0.05). There was a correlation between the overexpressison of Fascin-1 protein, the nuclear expression of β-catenin and the mutation of K-ras gene (rs= 0.252, rs= 0.258, P < 0.05). The overexpression of Fascin-1 protein positively correlated with the nuclear expression of β-catenin (rs= 0.213, P < 0.05). Conclusion Fascin-1 protein and β-catenin protein are involved in invasion and metastasis of colorectal cancer and are associated with K-ras gene mutation. K-ras may promote the overexpression of Fascin-1 by virtue of nuclear expression ofβ-catenin, which provided a new research direction on the treatment of K-ras gene mutated colorectal adenocarcinoma.
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Objective To explore the expression of Fascin-1 and EGFR in triple-negative breast cancer (TNBC) and non-triple-negative breast cancer (non-TNBC) and its correlation.Methods According to ER,PR,and HER2 status,breast cancer were categorized into 2 subtypes:70 cases of TNBC and 370 cases of non-TNBC.The immunohistochemical technique,EnVision method,was used to evaluate the expression of Fascin-1 and EGFR in breast cancer.Results Expression rate of Fascin-1 and EGFR protein in TNBC was 88.6％(62/70)and 78.6％(55/70),while it was 19.2％(71/370)and 44.3％(164/370)in non-TNBC,respectively.Fascin-1 expression rate was significantly higher in EGFR positive non-TNBC cases (34.8％,57/164) than in EGFR negative cases (6.8％,14/206)(x2=46.032,P=0.000).The positive rate of Fascin-1 protein in EGFR-positive TNBC cases (92.7％,51/55) was higher than that in EGFR negative cases (73.3％,11/15),and the difference had no statistically significance (x2=2.673,P=0.102).Conclusions EGFR signal pathway may positively regulate Fascin-1 expression in non-TNBC.The relationship between EGFR and Fascin-1 in TNBC is needed for further study.
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Objective To investigate the expression of Fascin-1 in gastric carcinoma and its association with clinicopatho-logical features. Methods A total of 98 gastric cancer specimens were collected from our hospital from August 2014 to De-cember 2016.The expression of Fascin-1 mRNA and protein was detected in gastric cancer cell lines(MKN28,MKN45 and AGS)and gastric cancer tissues by real-time PCR,Western blotting and immunohistochemistry,respectively.The relationship between Fascin-1 protein expression and clinicopathological features was analyzed. Results Fascin-1 was highly expressed at mRNA and protein levels in MKN28 and MKN45 gastric cancer cell lines,and it was weakly expressed in AGS cell line.The ex-pression of Fascin-1 mRNA was much higher in gastric cancer tissues than in adjacent tissues.The expression of Fascin-1 was correlated with invasive depth,lymph node metastasis,lymphatic invasion,tumor differentiation and TNM stage(all P<0.05). Conclusion Fascin-1 is overexpressed in gastric cancer tissues.It is associated with the progression,invasion and malignancy of gastric cancer.It is expected to become a molecular marker for the progression of gastric cancer.
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Objective To investigate the expression of Fascin‐1 in colorectal cancer (CRC) and its clinical significance .Meth‐ods Immunohistochemistry MaxVision method was adopted to detect the protein expression of Fascin‐1 in 87 CRC tissues and 28 para‐cancer tissues ,and analyzed the expressions of them and the relations to clinicopathologic characteristics .Kaplan‐Meier plots and Cox proportional hazards regression model were used to analyze the prognostic value of Fascin‐1 expression .Results The posi‐tive rates of Fascin‐1 expression in CRC tissues and para‐cancer tissues were 43 .7% (38/87) and 7 .1% (2/28) respectively ,with significant difference (P0 .05) .In the Kaplan‐Meier analysis ,patients with Fascin‐1 over‐expression had significantly shorter overall survival than those patients with negative Fascin‐1 expression (P=0 .009) .Multivari‐able analysis by Cox regression model further showed that Fascin‐1 over‐expression was a significantly independent predictor of poorer overall survival (HR=2 .087;95% CI:1 .196-3 .642 ,P=0 .010) .Conclusion The expression of Fascin‐1 is high and its re‐lated to the long‐term survival time of patients with CRC .It is an independent prognostic factor for CRC .
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Objective:To detect the functional role of Fascin1 and its related molecular mechanisms in migration and invasion capacity of glioma cells,we utilized gene specific small interference RNA of Fascin1 in cell line U87 MG. Methods:Fascin1-siRNA or negative siRNA was transfected into U87 MG cells of control group or experiment group. Transwell method was employed to assess the migration and invasion capacity of glioma cells. Western blot analysis was used to detect the protein expression of Fascin1,pAKT and pSTAT3. The impact of PI3K/AKT pathway and STAT3 pathway on migration and invasion of U87 MG cells was verified,via applying LY294002 and LY294002,which was inhibitor of the two pathways respectively. Results:As compared to control groups,the migration and invasion capacity of transfected glioma cells were attenuated about 52% or 43%(P<0. 05),accompanied with the decreased phos-phorylation of AKT and STAT3. As utilizing the inhibitors of AKT and STAT3,attenuated migration and invasion capacity of U87 MG cells were observed. Conclusion:Down-regulated expression of Fascin1 could suppress the migration and invasion capacity of U87 MG cells by inhibiting the phosphorylation of PI3K/AKT pathway and STAT3 pathway.
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Purpose To observe the mutation of K-ras gene and expression of Fascin-1 protein in CRC tissues and their relationship with clinical pathological features, and then to analyze the correlation between mutation of K-ras and expression of Fascin-1. Methods In 86 cases of CRC tissues, K-ras mutation was detected by DNA sequencing analysis, and Fascin-1 expression was detected by im-munohistochemical method. Results In CRC tissues the mutation rate of K-ras was 34. 88%, the expression rate of Fascin-1 was 60. 47%. The mutation rate of K-ras in lymph node metastasis group was higher than that of without lymph node metastasis group, and that in distant metastasis group was higher than that of without distant metastasis group(P<0. 05). The expression rate of Fascin-1 in serosa invasion group was higher than that of without serosa invasion group, and that in lymph node metastasis group was higher than that of without lymph node metastasis group, and that in distant metastasis group was higher than that of without distant metastasis group (P<0. 01). There was a correlation between the mutation of K-ras gene and the expression of Fascin-1 in CRC tissues (rp =0. 236, P<0. 05). Conclusions The CRC tissues with mutation of K-ras are more likely to metastasize and the CRC tissues with expression of Fascin-1 are more likely to invade serosa and metastasize. The CRC tissues with mutation of K-ras are more likely to express Fascin-1.
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Objective To investigate the relationship of fascin-1 protein expression with the metastasis of basal cell carcinoma and squamous cell carcinoma. Methods Skin specimens were obtained from 10 normal human controls, 13 patients with basal cell carcinoma (8 nodular variant and 5 superficial variant) and 24 patients with SCC (11 SCC in situ and 13 invasive SCC). Immunohistochemical staining was performed to analyze the expression of fascin-1 protein. The staining results were quantitatively assessed with computer image analysis system (Image-pro Plus 6.0). Results The optical density of fascin-1 averaged 0.1152±0.04574 in SCC in situ, 0.1257±0.03096 in invasive SCC, and 0.0293±0.00981 in normal controls; the expression of fascin-1 was significantly higher in SCC tissue than in normal control skin (both P<0.05). Increased optical density was also observed for fascin-1 in nodular variant of SCC (0.0808 ±0.05642) and superficial variant of SCC (0.0806±0.04346) compared with the normal controls, whereas no statistical difference was observed between nodular and superficial variant of SCC. Conclusion In BCC and SCC, there is an over expression of fascin-1, which may be linked to the local invasion of carcinoma,
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Objective To investigate the expression of Fascin-1 protein in colorectal adenocarcinoma,and the relationship with its clinicopathologic features.Methods The expressions of Fascin-1 protein in colorectal adenocarcinoma tissues of 60 cases,colorectal adenoma tissues of 30 cases and normal mucosa tissues (4 cm distance to neoplasm) of 30 cases were detected by Microwave-EliVisionTM immunohistochemistry method,and the relationship between the expression of Fascin-1 protein in colorectal adenocarcinoma tissues and its clinicopathologic characteristics was analyzed.Results The expression of Fascin-1 protein was located in cytoplasm.The positive expression rates of Facsin-1 protein were 3.3% (1/30),30.0% (9/30) and 53.3% (32/60) in normal mucosa tissues,colorectal adenoma tissues and colorectal adenocarcinoma tissues,respectively.The expression of Fascin-1 was gradually increased in these three tissues,and there was statistical difference among the three tissues (P0.05).Conclusion The high expression of Fascin-1 protein is correlated to high invasion ability and lymph node metastasis,which can play as a sensitive index in predicting the invasion and metastasis of colorectal adenocarcinoma.