Isolation and characterization of native Rhizobium from root nodules of raikia french bean growing area of Odisha-Supplementary Data

Injudicious application of nitrogenous fertilizers leads to soil quality deterioration which results into yield loss. The application of biofertilizer containing native efficient rhizobia enhances the nodular properties, N-fixation and soil quality. Therefore, fifty strains of Rhizobium were isolated from root nodule of Raikia french bean and among them only two isolates viz., RBHR-15 and RBHR-21 were confirmed as Rhizobium. The isolates were unable to grow under anaerobic conditions and failed to produce ketolactase enzyme, showed a negative response for gelatin liquefaction and Simmon’s citrate agar test, responded positively towards the indole test, MR-VP, TSI test and could produce NH4+ from peptone in the growth medium. The isolate RBHR-15, could reduce H2S and nitrate whereas, RBHR-21 could not. The growth of both isolates was luxuriant in the nutrient broth containing 1% NaCl and decreased with an increase in the concentration of NaCl and grew profusely in the pH range of 6-8. The generation time of RBHR-15 and RBHR-21 were 16.4and 10.6 h, respectively. The 16s rRNA of both isolates was sequenced and submitted to the National Center for Biotechnology Information (NCBI). The isolates RBHR-15, and RBHR-21 were assigned accession numbers MN480514 and MN480516.

Isolation and characterization of native Rhizobium from root nodules of raikia french bean growing area of Odisha

Injudicious application of nitrogenous fertilizers leads to soil quality deterioration which results into yield loss. The application of biofertilizer containing native efficient rhizobia enhances the nodular properties, N-fixation and soil quality. Therefore, fifty strains of Rhizobium were isolated from root nodule of Raikia french bean and among them only two isolates viz., RBHR-15 and RBHR-21 were confirmed as Rhizobium. The isolates were unable to grow under anaerobic conditions and failed to produce ketolactase enzyme, showed a negative response for gelatin liquefaction and Simmon’s citrate agar test, responded positively towards the indole test, MR-VP, TSI test and could produce NH4+ from peptone in the growth medium. The isolate RBHR-15, could reduce H2S and nitrate whereas, RBHR-21 could not. The growth of both isolates was luxuriant in the nutrient broth containing 1% NaCl and decreased with an increase in the concentration of NaCl and grew profusely in the pH range of 6-8. The generation time of RBHR-15 and RBHR-21 were 16.4and 10.6 h, respectively. The 16s rRNA of both isolates was sequenced and submitted to the National Center for Biotechnology Information (NCBI). The isolates RBHR-15, and RBHR-21 were assigned accession numbers MN480514 and MN480516.

COVID-19 epidemic in remote areas of the French Amazon, March 2020 to May 2021: Another reality

ABSTRACT Background: French Guiana (FG) is an ultra-peripheral European region in the Amazon, and the COVID-19 epidemic has had very different kinetics from both its giant neighbors, Brazil or mainland France. Methods: This study summarized the epidemics of COVID-19 in FG. Results: The tropical climate, multiethnicity, and remoteness of the population forced healthcare providers to accordingly adapt the management of the epidemic. Incidence and mortality have been lower than that in Europe and Latin America due to a combination of prevalence of the youth in the population and highly developed healthcare system. Conclusions: Currently, vaccine hesitancy hinders the rapid expansion of vaccine coverage.

High incidence of acute Q fever among incarcerated people in Cayenne, French Guiana

ABSTRACT Q fever is a major public health problem in French Guiana. In recent years, a considerable number of cases has been reported in French Guiana's penitentiary center. The main objective of this study was to describe the epidemiology of these cases. A retrospective study was conducted at the prison to identify cases of acute Q fever in people incarcerated between 2010 and 2021. During the study period, 16 patients were diagnosed with acute Q fever. The positivity rate varied between 13 and 57%. The annual incidence rate in 2019, 2020 and 2021 was 269 (95% CI: 0-640) 1,120 (95% CI: 290-1950) and 1,931 (95% CI: 60-3810) per 100,000 person-years, respectively. While several vertebrate species have already been shown to play an important role in the transmission of Coxiella burnetii, the full epidemiology picture in the tropics is far from clear, and the prison context, with its controlled environment, could help provide answers.

Interpretation and thoughts on French Endocrine Society consensus statement on management of thyroid dysfunctions in the elderly / 中华内分泌代谢杂志

In 2020, the French Endocrine Society released a consensus statement on the management of thyroid dysfunction in the elderly. This consensus introduced the physiological evolution of thyroid aging, determined specific reference range of thyroid stimulating hormone in the elderly and provided a standard guidance for diagnosis and treatment of thyroid dysfunction. However, some of these recommendations are still controversial, especially the reference range of thyroid stimulating hormone and interventions for subclinical hypothyroidism in the elderly. Thus, further studies for the elderly in China are reguired in order to promote the standardized management of thyroid dysfunction.

Potential health benefits of resveratrol: a pharmacokinetics-caused conundrum / 中国临床药理学与治疗学

Resveratrol (3, 5, 4'-trihydroxy-trans-stilbene) was first identified from white hellebore (Veratrum grandiflorum) root and began to attract interest when its presence in red wine and cardiovascular activities were reported, leading to speculation of its contribution to the 'French paradox'. Besides the cardiovascular protection, potential health benefits of resveratrol include calorie restriction-like effects, cancer prevention and adjunctive therapy, and neuroprotection. In order to achieve translational applications of these potential benefits, pharmacokinetic research was performed for plasma pharmacokinetics and related disposition of orally dosed resveratrol. This paper summarizes the known human pharmacokinetic characteristics of resveratrol after oral administration and various attempts to improve its systemic exposure level from the perspectives of systemic exposure and in vivo process. However, available pharmacokinetic data of resveratrol has raised conundrums that limit translating potential benefits to clinics: (1) differences between the unchanged resveratrol used in bioactivity studies and its major circulating forms (i.e., metabolites) after dosing; (2) resveratrol's test concentrations used to exert in vitro bioactivities related to the benefits significantly higher than the compound's clinically achievable concentrations; (3) resveratrol's concentrations achievable (estimated from the pharmacokinetics) from doses used to produce in vivo efficacy significantly lower than the effective concentrations found in studies of related action mechanism (suggesting unreliability of test mechanism). In the last part of this review, we provide recommendations for future pharmacokinetic investigations of resveratrol, including a more systematic investigation of systemic exposure to resveratrol metabolites, their access to in vivo loci responsible for the benefits, and their disposition in target cells; an investigation of colon-luminal exposure to resveratrol and its metabolites for accessing colonic microbiota; and a multi-compound pharmacokinetic investigation of red wine.

Ideal temperature and storage period for commercial potato cultivars selected for frying / Temperatura e período de armazenamento ideais para cultivares comerciais de batata selecionadas para fritura

ABSTRACT: Potatoes for industrial processing must have high dry matter, low sugar and free from damage or disease. The objective was to determine the ideal temperature and storage period of commercial cultivars for frying. Tubers of Asterix and Cronos cultivars were stored in a cold chamber (Gallant CMC4 Premium) inside plastic boxes at 6 and 8 °C with 85 to 95% humidity for 180 days. Accumulated mass loss (PMA), alcohol insoluble solids (SIA), total soluble sugars (AST), non-reducing sugars (ANR), reducing sugars (AR), polyphenoloxidase activity (PPO) and enzymatic and non-enzymatic browning were analyzed. The PMA of Asterix at 6 and 8 ºC and Cronos at 6 °C was higher. The SIA of both cultivars stored at 6 ºC were lower and AST, AR and ANR higher. Those parameters of Cronos and Asterix did not differ between temperature or storage period. The browning was greater in the fried sticks of Asterix and Cronos stored at 6 ºC (4 to 5) for 60 and 90 days and at 8 °C (2 to 3) for 180 days, respectively. The ideal temperature and storage period for Asterix and Cronos cultivars is 8 ºC for a maximum of 120 days due to non-enzymatic browning.

RESUMO: As batatas destinadas ao processamento industrial devem ter alto teor de matéria seca, baixo teor de açúcar e estar livre de danos ou doenças. O objetivo foi determinar a temperatura e o período de armazenamento ideais para cultivares comerciais para fritura. Os tubérculos das cultivares Asterix e Cronos foram armazenados em caixas de plástico a 6 e 8 °C com umidade de 85 a 95% por 180 dias. As perdas de massa acumulada (PMA), sólidos insolúveis em álcool (SIA), açúcares solúveis totais (AST), açúcares não redutores (ANR), açúcares redutores (AR), atividade de polifenoloxidase (PPO) e escurecimento enzimático e não enzimático foram analisadas. A PMA de tubérculos da cultivar Asterix a 6 e 8 ºC e a da cultivar Cronos, armazenadas a 6 °C foram maiores. Os teores de SIA foram menores e os de AST, AR e ANR maiores nos tubérculos de ambas as cultivares armazenadas a 6 ºC. A temperatura e o período de armazenamento não afetaram as cultivares Cronos e Asterix. O escurecimento foi maior nos palitos fritos das cultivares Asterix e Cronos armazenados a 6 ºC (4 a 5) por 60 e 90 dias e a 8 °C (2 a 3) por 180 dias, respectivamente. O período ideal de temperatura e armazenamento para as cultivares Asterix e Cronos é de 8 ºC por, no máximo, 120 dias devido ao escurecimento não enzimático.

Critical care medicine in the French Territories in the Americas: Current situation and prospects / La medicina intensiva en los territorios franceses de la Región de las Américas: situación actual y perspectivas / Medicina intensiva nos territórios ultramarinos franceses nas Américas: situação atual e perspectivas

ABSTRACT Hospitals in the French Territories in the Americas (FTA) work according to international and French standards. This paper aims to describe different aspects of critical care in the FTA. For this, we reviewed official information about population size and intensive care unit (ICU) bed capacity in the FTA and literature on FTA ICU specificities. Persons living in or visiting the FTA are exposed to specific risks, mainly severe road traffic injuries, envenoming, stab or ballistic wounds, and emergent tropical infectious diseases. These diseases may require specific knowledge and critical care management. However, there are not enough ICU beds in the FTA. Indeed, there are 7.2 ICU beds/100 000 population in Guadeloupe, 7.2 in Martinique, and 4.5 in French Guiana. In addition, seriously ill patients in remote areas regularly have to be transferred, most often by helicopter, resulting in a delay in admission to intensive care. The COVID-19 crisis has shown that the health care system in the FTA is unready to face such an epidemic and that intensive care bed capacity must be increased. In conclusion, the critical care sector in the FTA requires upgrading of infrastructure, human resources, and equipment as well as enhancement of multidisciplinary care. Also needed are promotion of training, research, and regional and international medical and scientific cooperation.

RESUMEN Los hospitales en los territorios franceses de la Región de las Américas funcionan según las normas francesas e internacionales. El objetivo de este artículo es describir distintos aspectos de los cuidados intensivos en los territorios franceses. Para ello, hemos revisado los datos oficiales sobre el tamaño de la población y el número de camas de las unidades de cuidados intensivos (UCI), así como la bibliografía sobre algunos aspectos específicos de las UCI, en los territorios franceses. Las personas que viven en los territorios franceses, o que están de visita en ellos, están expuestas a riesgos específicos: principalmente traumatismos graves causados por el tránsito, envenenamiento por mordeduras, heridas de bala o por apuñalamiento, y enfermedades infecciosas tropicales emergentes. La atención de estos traumatismos y enfermedades puede requerir conocimientos específicos y cuidados intensivos. Sin embargo, no hay suficientes camas de UCI en los territorios franceses. De hecho, hay 7,2 camas de UCI por 100 000 habitantes en Guadalupe, 7,2 en Martinica y 4,5 en Guayana Francesa. Además, los pacientes gravemente enfermos que viven en zonas remotas a menudo tienen que ser trasladados, normalmente por helicóptero, lo que retrasa su ingreso en la unidad de cuidados intensivos. La crisis de la COVID-19 ha puesto de manifiesto que el sistema de atención de salud en los territorios franceses no está preparado para enfrentarse a una epidemia de estas dimensiones y que debe aumentarse la capacidad hospitalaria de las unidades de cuidados intensivos. En conclusión, el sector de los cuidados intensivos en los territorios franceses tiene que mejorar su infraestructura, recursos humanos y equipamiento, así como perfeccionar la atención multidisciplinaria. También es necesario promover la capacitación, la investigación y la cooperación médica y científica, tanto regional como internacional.

RESUMO Os hospitais nos territórios ultramarinos franceses nas Américas funcionam segundo os padrões franceses e internacionais. O objetivo deste artigo é descrever os diversos aspectos da atenção intensiva nesta região. Analisamos os dados oficiais relativos ao tamanho da população e ao número de leitos de unidade de terapia intensiva (UTI) nestes territórios junto com uma revisão da literatura científica sobre as características particulares destes centros de terapia intensiva. Os residentes locais ou visitantes dos territórios ultramarinos franceses nas Américas são expostos a riscos específicos, sobretudo acidentes de trânsito graves, envenenamentos por animais peçonhentos, ferimentos por armas brancas ou armas de fogo e doenças infecciosas tropicais emergentes que requerem conhecimento especializado e atenção intensiva. Porém, não há leitos suficientes de UTI nos territórios ultramarinos franceses nas Américas: são 7,2 leitos de UTI por 100.000 habitantes em Guadalupe, 7,2 na Martinica e 4,5 na Guiana Francesa. Ademais, em áreas remotas, os pacientes em estado crítico frequentemente precisam ser transferidos por helicóptero, o que causa demora na internação em UTI. A crise da COVID-19 demonstra o despreparo do sistema de saúde para enfrentar a pandemia e a necessidade de aumentar o número de leitos de UTI nestes territórios. Em conclusão, é imprescindível modernizar a infraestrutura e os equipamentos, capacitar melhor os recursos humanos e melhorar a atenção multidisciplinar. Incentivar a formação profissional, pesquisa e cooperação médico-científica regional e mundial é também fundamental.

High prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae in Western French Guiana

ABSTRACT The purpose of this study was to estimate the prevalence of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections in western French Guiana and to analyze associated factors with both infections. A retrospective study was conducted in a sexually transmitted infections clinic in 2017. Women (n = 338) were tested by real-time polymerase chain reaction for vaginal, anal and throat CT and NG infections. Men (n = 192) were evaluated using urine specimens. Socio-demographic and sexual behaviour data were collected by nurses with a structured questionnaire. The prevalence of CT and NG infections among women were 24.3% and 13.3%, respectively, and 12.0% and 7.3% among men. Women aged under 25 years had a higher risk of CT and NG infections than women aged 35 years or more. Another risk factor for CT infection was low educational level, and occasional unprotected sex for NG infection. CT and NG infections were associated with reporting symptoms among men. Very high prevalences of CT and NG infections among women and men were found, which suggest that a large-scale screening strategy should be implemented in French Guiana.

Spatiotemporal multiple insecticide resistance in Aedes aegypti populations in French Guiana: need for alternative vector control

BACKGROUND Aedes aegypti is the sole vector of urban arboviruses in French Guiana. Overtime, the species has been responsible for the transmission of viruses during yellow fever, dengue, chikungunya and Zika outbreaks. Decades of vector control have produced resistant populations to deltamethrin, the sole molecule available to control adult mosquitoes in this French Territory. OBJECTIVES Our surveillance aimed to provide public health authorities with data on insecticide resistance in Ae. aegypti populations and other species of interest in French Guiana. Monitoring resistance to the insecticide used for vector control and to other molecule is a key component to develop an insecticide resistance management plan. METHODS In 2009, we started to monitor resistance phenotypes to deltamethrin and target-site mechanisms in Ae. aegypti populations across the territory using the WHO impregnated paper test and allelic discrimination assay. FINDINGS Eight years surveillance revealed well-installed resistance and the dramatic increase of alleles on the sodium voltage-gated gene, known to confer resistance to pyrethroids (PY). In addition, we observed that populations were resistant to malathion (organophosphorous, OP) and alpha-cypermethrin (PY). Some resistance was also detected to molecules from the carbamate family. Finally, those populations somehow recovered susceptibility against fenitrothion (OP). In addition, other species distributed in urban areas revealed to be also resistant to pyrethroids. CONCLUSION The resistance level can jeopardize the efficiency of chemical adult control in absence of other alternatives and conducts to strongly rely on larval control measures to reduce mosquito burden. Vector control strategies need to evolve to maintain or regain efficacy during epidemics.

Lacan e o feminismo francês: a história de uma (não) relação / Lacan and French feminism: the story of a (non) relation / Lacan y el feminismo francés: la historia de una (no) relación / Lacan et le féminisme français: l´histoire d´un (non) rapport

Resumo Este trabalho apresenta as teorias empreendidas pelas feministas francesas dos anos 1970, notadamente a respeito do uso e da interpretação que fizeram da psicanálise lacaniana, assim como o que consideramos ter sido a postura epistemológica que Lacan adota nesse momento. Fortemente marcadas por Derrida e o pós-estruturalismo, Irigaray e Cixous tecem suas críticas ao arsenal teórico psicanalítico supostamente condizente com o modelo patriarcal, ao passo que concebem um tipo de escrita subversiva com aspirações políticas. Também nesse intuito, Montrelay e Kristeva pretendem oferecer uma sustentação teórica consistente com o movimento. Já Wittig, marcada pelo marxismo, lê os conceitos lacanianos como determinados pela mentalidade heterossexual enquanto regime político. Sustentamos que Lacan, atento ao entusiasmo feminista do período, opta por desenvolver sua teoria da sexuação e se dedica à formalização do aforismo "Não há relação sexual".

Abstract This paper presents French feminist theories of the 1970s, especially regarding the use and interpretation they have made of Lacanian psychoanalysis, such as what we consider to have been the epistemological attitude that Lacan adopted at that moment. Substantially influenced by Derrida and poststructuralism, Irigaray and Cixous make their criticisms of the theoretical psychoanalytic arsenal that supposedly matched the patriarchal model, while conceiving a kind of subversive writing with political aspirations. Also for this purpose, Montrelay and Kristeva offer a consistent theoretical support to this movement. Wittig, marked by Marxism, interprets Lacanian concepts as determined by heterosexual mentality as a political regime. We think that Lacan, attentive to the feminist enthusiasm of this period, chooses to develop his theory of sexuation and dedicates itself to the formalization of the aphorism "There is no sexual relation".

Resumen Este artículo pretende presentar las teorías emprendidas por las feministas francesas de los años 70, especialmente sobre el uso y la interpretación que hicieron del psicoanálisis lacaniano, así como lo que consideramos haber sido la postura epistemológica que Lacan adopta en ese entonces. Marcadas fuertemente por Derrida y el postestructuralismo, Irigaray y Cixous tejen sus críticas al arsenal teórico psicoanalítico supuestamente concordante con el modelo patriarcal, mientras que conciben un tipo de escritura subversiva con aspiraciones políticas. También en este propósito, Montrelay y Kristeva pretenden ofrecer una fundamentación teórica consistente en este movimiento. Wittig, marcada por el marxismo, considera los conceptos lacanianos determinados por la mentalidad heterosexual como régimen político. Sostenemos que Lacan, atento al entusiasmo feminista de ese período, opta por desarrollar su teoría de la sexuación y se dedica a la formalización del aforismo "No hay relación sexual".

Résumé Cet article vise à présenter les théories des féministes françaises des années 1970, notamment en ce qui concerne l'utilisation et l'interprétation de la psychanalyse lacanienne, ainsi que ce que nous considérons comme la position épistémologique adoptée par Lacan en ce moment. Fortement marquées par Derrida et le post-structuralisme, Irigaray et Cixous posent leurs critiques de l'arsenal théorique psychanalytique supposée correspondant au modèle patriarcal, tout en concevant une sorte d'écriture subversive avec des aspirations politiques. Aussi à cet effet, Montrelay et Kristeva ont l'intention d'offrir un soutien théorique cohérent à ce mouvement. Wittig, marqué par le marxisme, lit les concepts lacaniens tels que déterminés par la mentalité hétérosexuelle en tant que régime politique. Nous soutenons que Lacan, attentif à l'enthousiasme féministe de cette période, choisit de développer sa théorie de la sexuation et se consacre à la formalisation de l'aphorisme "Il n'y a pas de rapport sexuel".

3D printing as a diagnostic tool for congenital malformation in a brachycephalic dog / Impressão 3D como ferramenta de diagnóstico para malformação congênita em cão braquicefálico

ABSTRACT: Congenital anomalies are hereditary or acquired, and their location and intensity are determining factors for the survival of animals. Some cases are rare, often unidentified, poorly reported and of unknown etiology. This paper reports a congenital malformation in a French bulldog, demonstrating the importance of accurate diagnosis for surgical decisions. The use of new technologies such as computed tomography and rapid prototyping enables the analysis of morphofunctional changes, resulting in excellent results for clinical cases in which it is difficult to identify and scale the anatomical deformities. Through this feature, it is possible to accurately recreate anatomical structures of interest, enabling greater assertiveness in deciding the treatment to be established, whether surgical or not. Castration of animals that survive this condition due to hereditary etiology is recommended.

RESUMO: Anomalias congênitas possuem caráter hereditário ou adquirido, sendo que sua localização e intensidade são fatores determinantes para a sobrevivência do animal. Algumas são raras, muitas vezes não identificadas, pouco relatadas e com etiologia desconhecida. O presente trabalho registra um caso de malformação congênita em um bulldog francês, demonstrando a importância do diagnóstico preciso para a decisão cirúrgica. A utilização de novas tecnologias como tomografia computadorizada e prototipagem rápida possibilitam a análise de alterações morfofuncionais, tendo ótimo resultado para casos clínicos em que há dificuldade em identificar e dimensionar deformidades anatômicas. Através desse recurso é possível recriar com precisão estruturas anatômicas de interesse, possibilitando maior assertiva na decisão do tratamento a ser estabelecido, sendo ele cirúrgico ou não. É recomendada a castração dos animais que sobreviveram a essa condição, devido a etiologia hereditária.

Método Natural de Georges Hébert: princípios e primeiras influências (1905-1914)

RESUMO O artigo tem como objetivo analisar as influências que envolveram a criação do Método Natural de Georges Hébert e discutir sobre seus princípios. O Guide Pratique d'Éducation Physique (1909) é a fonte selecionada e a discussão historiográfica tem contribuição de autores como Philippe-Meden (2017), Froissart e Saint-Martin (2014). O recorte inicial marca sua primeira publicação e o final, o encerramento das atividades do Collège d'athlètes. Hébert idealizou um método para a formação dos fuzileiros navais da Marinha francesa e seu reconhecimento ultrapassou as fronteiras nacionais. Ele propunha o desenvolvimento orgânico harmônico por meio do grupo de dez exercícios que, praticados ao ar livre e em desnudamento, desenvolveriam força, coragem e sangue frio.

ABSTRACT The purpose of the article is to analyze the influences that involved the creation of the Natural Method of Georges Hébert and to discuss his principles. The Guide Pratique d'Éducation Physique (1909) is the book selected as source. The historiographical discussion is based on authors such as Philippe-Meden (2017), Froissart and Saint-Martin (2014). The initial clipping marks its first publication, and the last one is the closing of the activities of the Collège d'athlètes. Hébert devised a method for training the Marines of the French Navy and his fame became international. He proposed harmonious organic development through the group of ten exercises that practiced outdoors and in denudation would develop strength, courage and cold blood.

RESUMEN El artículo tiene como objetivo analizar las influencias que generaron la creación del Método Natural de Georges Hébert y discutir sobre sus fundamentos. La Guide Pratique d'Éducation Physique (1909) es la fuente seleccionada y la discusión historiográfica contiene aportaciones de autores como Philippe-Meden (2017), Froissart y Saint-Martin (2014). La línea de corte inicial marca su primera publicación y el final, el cese de las actividades del Collège d'athlètes. Hébert idealizó un método para la formación de los marineros de la Marina francesa y su reconocimiento sobrepasó las fronteras nacionales. Proponía el desarrollo orgánico armónico por medio del grupo de diez ejercicios que se practicaban al aire libre y al desnudo, los cuales desarrollarían fuerza, coraje y sangre fría.

Sobre a (homo)sexualidade nos meios de comunicação (1980-2010) / About (homo)sexuality in the media (1980-2010) / Sobre la (homo)sexualidad en los medios de comunicación (1980-2010)

Nesta nota de conjuntura, proponho mostrar um percurso da relação entre a homossexualidade e a imprensa de circulação nacional, aqui no Brasil. Como ponto de partida, abordo a emergência da Aids, no início dos anos de 1980, tendo em vista que para falar sobre a homossexualidade, os meios de comunicação recorriam aos discursos médico e religioso para discursivizar o homossexual/homossexualidade e finalizo nos anos 2010, quando, nestes mesmos veículos de informação, surgem outras relações para os sujeitos LGBTQI+. A partir da teoria francesa de análise do discurso, escrevo sobre o funcionamento da imprensa e os deslizamentos de sentidos nesses últimos anos de uma relação que nunca acaba: esses sujeitos sempre são objetos de consideração no discurso jornalístico.

In this note on current situation, I propose to demonstrate a path of the relation between homosexuality and the press of national circulation in Brazil. As a starting point, I speak of the emergence of AIDS in the early 1980s, given that in order to talk about homosexuality the media resorted to medical and religious discourses to enunciate about the homosexual/the homosexuality, and I finish in the 2010s, when, in the same information carriers, other relations arise for LGBTQI+ subjects. From the French theoretical approach of discourse analysis, I write about the operations of the press and the slip of meanings in recent years of a relationship that never ends: these subjects are always objects of consideration in journalistic discourse.

En esta nota de coyuntura, propongo mostrar un recorrido de la relación entre la homosexualidad y la prensa de circulación nacional, aquí en Brasil. Como punto de partida, hablo de la emergencia del sida, a principios de los años 1980, teniendo en cuenta que, para hablar sobre la homosexualidad, los medios de comunicación recurrían a los discursos médico y religioso para discursivizar el homosexual/la homosexualidad, y termino en los años 2010, cuando en estos mismos vehículos de información surgen otras relaciones para los sujetos LGBTQI+. A partir de la teoría francesa de análisis del discurso, escribo sobre el funcionamiento de la prensa y los deslizamientos de sentidos en estos últimos años de una relación que nunca termina: esos sujetos siempre son objetos de consideración en el discurso periodístico.

Impacto da Reforma Cabanis no ensino médico do Brasil: ensaio de arqueologia neofoucaultiana / The impact of Cabanis reform on medical education in Brazil: an essay in neo-Foucauldian archeology

Resumo O ensaio avalia o impacto da Reforma Cabanis na configuração do modelo de formação em saúde que se tornou hegemônico no Brasil. Primeiro, discute o processo de construção social, ideológica e institucional do modelo de assistência à saúde da França pós-revolucionária. Em seguida, introduz os principais elementos da Reforma Cabanis, analisando aspectos curriculares e pedagógicos da nova proposta de ensino médico baseado em profissionalismo, disciplinaridade e especialização que resultou num sistema de ensino superior sem universidades. Depois avalia o processo histórico que resultou no "afrancesamento" do sistema educacional brasileiro, resultando num modelo de formação em saúde baseado em faculdades, hospitais, aulas, disciplinas, especialidades e diplomas.

Abstract This text assesses the impact of the Cabanis reform on the formation of the health training model which became hegemonic in Brazil. First, we shall briefly discuss the process of constructing the social, ideological, and institutional framework for healthcare in post-revolutionary France. Next the main elements of the Cabanis reform are introduced, analyzing curricular and pedagogical aspects of the new plan for medical education based on professionalism, disciplines, and expertise that resulted in a system of higher education without universities. This is followed by assessment of the historical process which resulted in the "Francization" of the Brazilian educational system, particularly in higher education and more specifically medical education, producing a model of health training based on colleges, hospitals, classrooms, disciplines, skills, and diplomas.

Advances in diagnostic classification of erythroid proliferations in myeloid neoplasms / 白血病·淋巴瘤

Erythroid proliferations in myeloid neoplasms (MN-EP) is a group of heterogeneous diseases characterized by nucleated red blood cell proliferation and ineffective hematopoiesis in the bone marrow. Initial French-American-British (FAB) classification was used to diagnose and classify MN-EP according to morphological criteria. However, with the development of flow cytometry, cytogenetics and molecular biology techniques, FAB classification is obviously insufficient, which can not meet the clinical needs. Therefore, the World Health Organization (WHO) classification has introduced more accurate diagnostic classification standards and revised them several times. This paper reviews the research progress of MN-EP diagnostic classification.

Leigh syndrome (French Canadian type) caused by compound heterozygous mutations of LRPPRC gene: a case report / 中华神经科杂志

Leigh syndrome (French-Canadian type, LSFC) is a rare autosomal recessive hereditary severe neurological disorder that begins in infancy. Herein we report a case with LSFC in China. The patient was 8 months old, male, whose clinical manifestations included delayed development, low muscle tone, unstable vertical head, inability to sit alone, cognitive impairment, slightly smaller forehead, oblique eyes, epilepsy, etc. Gene sequencing results showed that the LRPPRC gene in the infant had complex heterozygous mutations of c.2989G>A (newly reported) and c.4078G>A. Combined with the clinical manifestations, gene mutations and literatures, the infant was diagnosed as LSFC, and symptomatic rehabilitation was performed. The results of genetic testing can contribute to the early diagnosis and genetic counseling of LSFC patients, and help reduce the burden on the patients and their families.

El impacto de la reforma Cabanis en la formación histórica de la universidad argentina y en la educación superior en salud / The impact of the Cabanis reform on the historical formation of the Argentine university and on higher education in health

RESUMEN En este ensayo, proponemos evaluar el impacto de la reforma Cabanis en la configuración del modelo de formación en salud que se hizo hegemónico en Argentina. Primero, analizamos la reestructuración del sistema educacional francés desencadenada por la revolución de 1789, principalmente en sus dimensiones político-institucionales. Segundo, discutimos brevemente el proceso de reconstrucción social, ideológica e institucional del sistema de salud en la Francia postrevolucionaria. Tercero, introducimos la reforma Cabanis, propuesta de enseñanza médica basada en profesionalismo, disciplinariedad y especialización que resultó en un sistema de enseñanza superior sin universidades, e indagamos en torno a sus principales aspectos curriculares y pedagógicos. Finalmente evaluamos la secuencia de eventos y procesos que, durante todo el siglo XIX, con fuerte gravitación cabanisiana a través de la Idéologie, conformaron los rasgos principales tanto de la enseñanza de la salud y de la medicina como del modelo de educación superior basado en facultades que actualmente predomina en la República Argentina.

ABSTRACT In this essay, we seek to evaluate the impact of the Cabanis reform on the configuration of the health education model that became hegemonic in Argentina. First, we analyze the restructuring of the French educational system triggered by the revolution of 1789, primarily in its political and institutional dimensions. Second, we briefly discuss the process of the social, ideological and institutional reconstruction of the health system in post-revolutionary France. Third, we introduce the Cabanis reform, a proposal for medical education based on professionalism, disciplinarity and specialization that resulted in a higher education system without universities, looking into its main curricular and pedagogical aspects. Finally, we evaluate the sequence of events and processes that, throughout the 19th century, with strong Cabanisian influence through the Idéologie, shaped both the principal features of teaching health and medicine and the higher education model based on faculties currently predominant in the Argentine Republic.

Liaisons dangereuses: cross-border gene flow and dispersal of insecticide resistance-associated genes in the mosquito Aedes aegypti from Brazil and French Guiana

BACKGROUND In recent years, South America has suffered the burden of continuous high impact outbreaks of dengue, chikungunya and Zika. Aedes aegypti is the main mosquito vector of these arboviruses and its control is the only solution to reduce transmission. OBJECTIVES In order to improve vector control it is essential to study mosquito population genetics in order to better estimate the population structures and the geneflow among them. METHODS We have analysed microsatellites and knockdown resistance (kdr) mutations from a trans-border region in Amazonia between the state of Amapá (Brazil) and French Guiana (overseas territory of France), to provide further knowledge on these issues. These two countries have followed distinct vector control policies since last century. For population genetic analyses we evaluated variability in 13 well-established microsatellites loci in Ae. aegypti from French Guiana (Saint Georges and Cayenne) and Brazil (Oiapoque and Macapá). The occurrence and frequency of kdr mutations in these same populations were accessed by TaqMan genotype assays for the sites 1016 (Val/Ile) and 1534 (Phe/Cys). FINDINGS We have detected high levels of gene flow between the closest cross-border samples of Saint-Georges and Oiapoque. These results suggest one common origin of re-colonisation for the populations of French Guiana and Oiapoque in Brazil, and a different source for Macapá, more similar to the other northern Brazilian populations. Genotyping of the kdr mutations revealed distinct patterns for Cayenne and Macapá associated with their different insecticide use history, and an admixture zone between these two patterns in Saint Georges and Oiapoque, in accordance with population genetic results. MAIN CONCLUSIONS The present study highlights the need for regional-local vector surveillance and transnational collaboration between neighboring countries to assess the impact of implemented vector control strategies, promote timely actions and develop preparedness plans.

As monografias da escola de educação física do Espírito Santo: circulação e produção de saberes (1932-1939) / The monographs from the Espírito Santo school of physical education: knowledge circulation and production (1932-1939) / Las monografías de la escuela de educación física de Espírito Santo: circulación y producción de conocimientos (1932-1939)

Analisa o processo de elaboração de monografias na Escola de Educação Física do Espírito Santo para compreender a formação de professores, a circulação de saberes e sua materialização como dispositivo de controle dos conhecimentos incorporados. Utiliza como fontes os documentos presentes no Arquivo Permanente do Centro de Educação Física e Desportos da Universidade Federal do Espírito Santo, entre os anos de 1932 e 1939. Usa como referencial teórico e metodológico os conceitos de lutas de representações e circulação e o modelo do paradigma indiciário. Os indícios nos permitem compreender que as monografias eram um dispositivo que reforçava a utilização do Método Francês.Os alunos usaram os recursos disponíveis na escola, como livros e periódicos, para sua elaboração. Os temas presentes nos trabalhos referiam-se aos saberes apresentados para a formação dos professores e à forma como o Método Francês foi utilizado no Estado

This article examines the process of writing monographs at the Espírito Santo School of Physical Education to understand teacher training, knowledge circulation and its materialization as a device to control incorporated knowledge. As sources, it uses documents in the Permanent Archive of the Center for Physical Education and Sports of the Federal University of Espírito Santo between 1932 and 1939. Its theoretical and methodological framework are the concepts of representational struggles, circulation and the model of the index paradigm. The evidence allows us to understand that monographs reinforced the use of the French Method. To write them, students used resources available at the School such as books and journals. The works' topics were related to knowledge presented for teacher training and the way the French Method was used in the state

Analizar el proceso de elaboración de monografías en la Escuela de Educación Física de Espírito Santo para entender la formación del profesorado, la circulación de conocimientos y su materialización como dispositivo de control de los conocimientos incorporados. Utiliza como fuentes los documentos presentes en el Archivo Permanente del Centro de Educación Física y Deportes de la Universidad Federal de Espírito Santo, abarcando el periodo entre 1932 y 1939. Utiliza como referencia teórica y metodológica los conceptos de lucha de representaciones y circulación y el modelo del paradigma indiciario. Los indicios nos permiten comprender que las monografías eran un dispositivo que reforzaba la utilización del Método Francés. Los alumnos utilizaron los recursos disponibles en la escuela, como libros y revistas, para su elaboración. Los temas presentes en el trabajo se referían a los conocimientos presentados para la formación de los profesores y a la forma en que el Método Francés ha sido utilizado en Espírito Santo