ABSTRACT
Objective:To establish an HPLC method with dual wavelengths for simultaneously determining chlorogenic acid , caffe-ic acid and paeoniflorin in Fufang Fuqing lotion .Methods: The HPLC method was performed on an Inertsil C 18 column ( 250 mm × 4.6 mm, 5 μm)with the column temperature at 40℃.The mobile phase was acetonitrile-0.02％ phosphoric acid (17:83) with the flow rate of 1.0 ml· min-1 .The detection wavelengths were set at 323 nm and 230 nm.Results:The calibration curve of chlorogenic acid, caffeic acid and paeoniflorin showed a good linear relationship over the range of 7.50-120.00 μg· ml-1(r=0.9999), 2.50-40.00 μg· ml-1(r=0.9998) and 14.06-225.00 μg· ml-1(r=0.9997), respectively.The average recovery was 98.55％ (RSD=1.66％,n=6), 94.52％(RSD=0.98％,n=6)and 99.18％(RSD=0.65％,n=6), respectively.Conclusion:The method is simple, quick, accurate and specific , which can be used for the quality analysis and control of Fufang Fuqing lotion .
ABSTRACT
Objective:To study the qualitative identification and quantitative analysis of Paeoniae radix Rubra in Fufang Fuqing lotion. Methods:TLC was used to identify Paeoniae radix Rubra. The content of paeoniflorinl was determined by HPLC. The mobile phase was acetonitrile-0. 01% phosphonic acid (13 ∶87), and the detection wavelength was 230 nm,the flow rate was 1. 0ml·min-1, the column temperature was 40℃,and the sample size was 10μl. Results:The results of TLC showed that the relevant spots were clear without any interference from the negative sample. The calibration curve of paeoniflorinl was linear within the range of 0. 070-4. 500 μg (r=1. 000 0). The average recovery was 98. 36% with RSD of 2. 73%(n=6). Conclusion:The methods are accurate and quick in the qualitative identification and quantitative assay of the preparation, which can be used for the quality control of Fufang Fuqing lo-tion.