ABSTRACT
Objective To investigate the contents of major five functional components (echinacoside, verbascoside, galactitol, betaine, soluble polysaccharide, and extractums) in Cistanche deserticola harvested in spring and autumn from genuine producing area in Alax Banner of Inner Mongolia. Methods HPLC-UV was applied to measuring the contents of echinacoside and verbascoside. HPLC-ELSD was used for determining the contents of galactitol and betaine. UV-VIS was utilized for analyzing the content of soluble polysaccharide. Results The index components of two samples' harvested in spring and autumn were all up to the standard of Chinese Pharmacopoeia. The samples harvested in spring contain 12.21 mg/g, which was twice of the autumn samples and fourfold of the standard of Chinese Pharmacopoeia. Based on verbascoside and betaine, the content of spring samples was significantly higher than the autumn samples, which was up to thirtyfold and it had great fluctuation among samples; Based on soluble polysaccharide, the content of spring and autumn samples were all at a high level, especially autumn samples was up to 13.7%; Based on galactitol and extractums, the content of autumn samples was significantly higher than the spring samples. Conclusion The content of verbascoside in C. deserticola has great fluctuation among samples. C. deserticola, that is rich in galactitol and soluble polysaccharide that authentic inner quality characteristics are "Glossy, Heavy, Fleshy, Quality soft, Sweet". Nevertheless, the ample galactitol and soluble polysaccharide is the material basis of quality formation, it is more reasonable to add the soluble polysaccharide as one of the index component to evaluate its quality characteristics. The standard of Chinese Pharmacopoeia points out the moisture limit of succulent herb is 10% may not be reasonable. The further improvement of the standard of C. deserticola's quality or separation of C. deserticola was discussed in this manuscript.
ABSTRACT
Objective: To analyze the crude drug of Cistanche deserticola from different sources and to supply some references for the quality control, collection, and processing of C. deserticola. Methods: HPLC-UV method was used to determine the contents of echinacoside and verbascoside, while HPLC-ELSD method was used to determine the content of galactitol. According to the content differences of these compounds, the influences of habitat, processing method, growing period, and space flight on the quality of C. deserticola had been discussed. Results: There were great differences among the samples from various sources. The quality of the crude drugs from the genuine producing areas, such as Inner Mongolia and Xinjiang was better than that from Ningxia. The influences of processing methods were different for the three constituents, the drying method for first killing the enzymes was better to echinacoside and verbascoside, the traditional drying method was better to galactitol. The contents of the active constituents in the crude drug underground > those growing above the ground before florescence > those in florescence > those after florescence. The content of verbascoside was higher in C. deserticola carried by space flight than that not carried by space flight, but the contents of echinacoside and galactitol were almost same. Conclusion: This method supplies a comprehensive analysis of C. deserticola, which could be used for the quality control, collection and processing of C. deserticola.
ABSTRACT
No abstract available.
Subject(s)
Humans , Infant , Galactitol , Galactosemias , Mass ScreeningABSTRACT
Escherichia coli mutants completely defective in galactose- 1-phosphate uridyl transferase (EC 2.7.7.10) and growing in glycerol medium undergo rapid cessation of growth when exposed to galactose. Toxicity due to galactose is equally pronounced when glycerol is replaced by other carbon sources, like succinate and proline. Gas chromatographic analysis failed to detect even trace amounts of galactitol. Moreover, galactose- 1-phosphate had no inhibitory role on some of the critical enzymes of cellular metabolism. General loss of energy (ATP) due to futile phosphorylation of galactose is probably the cause of bacteriostasis. The galT mutants can serve as models of human transferaseless galactosemia only to a limited extent.