ABSTRACT
TLC method for the identification of triterpenoids polyphenols in Fructus Chebulae was established .The results showed that this method had a plentiful chromatogram and good specificity,and can distinguish Fructus Chebulae immaturus from Fructus Chebulae.Determination of gallic acid, one of the hydrolysates of Fructus Chebulae, by HPLC was also reported.The linear range of gallic acid is from 0.17?g to 1.36?g(r=0.9998),and the average recovery is 102.9 %. The results showed that the method is simple, accurate and with good reproducibility.It can be used to determine gallic acid in Chinese herbal medicine that contains hydrolysable tannins.
ABSTRACT
Objective: A method for the determination of gallic acid in the extract of Quercus salece- na Blume (Fagaceae) and its preparation (Urocalum Capsules) by means of RP-HPLC was established. Method: The hydrolysable tannins contained in the sample is hydrolyzed with hy- drochloric acid, the hydrolysis component-gallic acid is determined by HPLC using RP-C18 column, 0.005mol. L-1 of phosphoric acid-methanol (98: 2) as mobile phase , 270 nm as detec- tion wavelength. Result: The linear range of gallic acid is 0. 3～3. 1?g , r=0. 9999, the average recovery is 100. 4%. Conclusion: It is confirmed that the established method for assay of gallic acid meet the criteria of quantitative analysis of herbal medicine by validation of hydrolysis condition, repeatebility, stability and recovery, The method is simple , good reproducible and precise , and it can also be applied to analysis of other herbal medicine containing hydrolysable tannins.
ABSTRACT
Objective:To set up a method of determining ga11ic acid content for the qua1ity contro1 of Shengjin Yanhou Tablet. Methods: The conditions of high performance liquid chromatography (HPLC)were as follow: Hypersi1 BDS as analytical co1umn, CH 3CN: 0.05 %H 3PO 4 (5∶95) as mobi1e phase and ultraviolet detector at 271nm. Results: A good linearity for ga11ic acid was in the range of 0.08 ～ 0.83 ?g.The repetition RSD was 2.46%, the precision RSD was 0.8% and the average recovery was 98.70%, RSD= 2.37%. Conclusion: The method was simp1e and can be used for the qua1ity control of Shengjin Yanhou Tablet.