Fractionation, structural characterization, and immunomodulatory activity of polysaccharide isolated from Ganoderma lucidum / 中草药

Objective To extract, separate, and purify polysaccharide from Ganoderma lucidum with alkali from the residue after water extraction, characterize the basic physicochemical properties and structural features in detail, and study the immunomodulatory activity in vitro. Methods The polysaccharide LZJ-015 was isolated and purified from the dry fruiting bodies of G. lucidum by alkaline extraction and ethanol precipitation following Q-Sepharose Fast Flow ion exchange chromatography column. Monosaccharide composition and molecular weight of LZJ-0.15 was analyzed by high performance liquid chromatography (HPLC) with PMP precolumn derivatization and high performance gel permeation chromatography-multiple angle laser (HPGPC-MALLS), respectively. The detailed structure of polysaccharide LZJ-0.15 was characterized by 1H-NMR, 13C-NMR, 1H-1H COSY, and 1H-13C HSQC spectrum. The immunomodulatory activity of G. lucidum polysaccharide was test by RAW264.7 cells phagocytose neutral red experiment. Results The molecular weight, molecular radius, and Mw/Mn of LZJ-0.15 were determined to be 24 700, 46.6 nm, and 1.019, respectively. The monosaccharide composition was confirmed to be mainly composed of glucose (92.3%). LZJ-0.15 was →3) Glc (β1→and→6) Glc (β1→linked glucan indicated by NMR spectrum. Moreover, G. lucidum polysaccharide exhibited good immunomodulatory activity in our study. Conclusion G. lucidum polysaccharide LZJ-0.15 from alkaline extraction showed better immune activity than the polysaccharide extracted from water, which would be potentially developed as an effective immunomodulatory agent.

Isolation and identification of active components of Ganoderma lucidum cultivated with grass and wood log I. Extraction, purification and characterization of glycopeptide / 中草药

Object To study the amount and properties of glycopeptide of Ganoderma lucidum (Leyss ex Fr ) Kaist. cultivated with grass (GLPG) and wood log (GLPW) ; to investigate the feasibility of substitution of grass for wood log to cultivate G lucidum Methods Glycopeptides were extracted and isolated from the fruit body of G lucidum followed by dialysis; the monosaccharid constituent and relative molecular weight of polysaccharides were examined by GC and gel filtration; structure of glycopeptide was determined by IR, 1H NMR and 13 C NMR Results Relative molecular weights of GLPW and GLPG glycopeptides were (Mw) 5 13?10 5 and 5 85?10 5, (M?) 6 21?10 5 and 6 96?10 5, respectively; the glucosidic bonds of both glycopeptides were ? type; both glycopeptides consist 16 amino acids and Rha, Xyl, Fru, Gal and Glc (there was trace amount of Man in GLPW) Conclusion There are less physical and chemical difference between GLPG and GLPW The purification rate of GLPG is 2 8 times to GLPW

Structure identification of triterpenes from fruiting bodies of Ganoderma lucidum by NMR spectra and X-ray diffraction analysis / 中草药

Object To study the chemical constituents from fruiting bodies of Ganoderma lucidum (Leyss ex Fr.) Karst Methods Individual constituent was isolated and repeatedly purified on silica gel column and structurally elucidated by the NMR spectra and X ray diffraction analysis Results Four compounds were obtained from the alcohol extract and identified as 3? oxo formyl 7?, 12? dihydroxy 4, 4, 14? trimethyl 5? chol 11, 15 dioxo 8 en (E) 24 oic acid (Ⅵ); 3? oxo formyl 7?, 12? dihydroxy 5? lanost 11, 15, 23 trioxo 8 en (E) 26 oic acid (Ⅶ); ergosterol (Ⅷ) and ergosta 7, 22 dien 3? ol (Ⅸ), respectively Conclusion Compound Ⅵ and Ⅶ are new triterpenes