ABSTRACT
A 1312 bp 5' flanking region of Salicornia europaea choline monooxygenase (SeCMO) gene was isolated using the anchored PCR. To investigate the mechanism of regulation for this stress-induced gene, the SeCMO promoter--glucuronidase (GUS) chimeric gene constructs containing five deletions F1, F2, F3, F4 and F5 were introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated transformation. The functional properties of each promoter fragment were examined by assaying GUS activity in the leaves of transgenic tobacco treated with abiotic stresses (NaCl, PEG6000 and low temperature). The GUS activity in transgenic tobacco with F2 (-1056 to +8) construct showed highest increase under all the three abiotic stresses. Thus, the study provided a potential promoter induced by the salt, dehydration and cold for the plant genetic manipulation.
Subject(s)
Base Sequence , Chenopodiaceae/genetics , Chenopodiaceae/metabolism , Cold Temperature , Glucuronidase/biosynthesis , Glucuronidase/genetics , Molecular Sequence Data , Oxygenases/genetics , Plant Proteins/genetics , Plant Proteins/metabolism , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , Polyethylene Glycols , Promoter Regions, Genetic/genetics , Sodium Chloride , Nicotiana/enzymology , Nicotiana/geneticsABSTRACT
To identify Escherichia coli through the production of â-D-glucuronidase (GUD), 622 suspect cultures were isolated from chicken carcasses and plated in PetrifilmTM EC. Of these cultures, only 44 (7.1 percent) failed to produce GUD. This result indicates the usefulness of GUD production for estimating E. coli populations in chicken.
Subject(s)
Animals , Enzyme Activation , Escherichia coli Infections , Escherichia coli/isolation & purification , Poultry , Food Samples , Methods , MethodsABSTRACT
OBJECTIVES: The purpose of this study was to get help in order to diagnose orthopaedic disease, measure its activity and determine treatment plan by measuring the beta-glucuronidase activity in urine, serum and joint fluid. METHODS: The beta-glucuronidase activity was determined in the serum, urine and joint fluid of the patients with degenerative arthritis, rheumatoid arthritis, osteomyelitis and osteogenic sarcoma, and some other disease to study the change of the enzyme activity. These values of each specimen were calculated by standard curve and treated by statistical analysis. RESULTS: The results obtained were summarized as follows. 1. The beta-glucuronidase activity in the serum, urine and joint fluid was increased in patients with degenerative arthritis, rheumatoid arthritis, osteomyelitis and osteogenic sarcoma etc. 2. The increased beta-glucuronidase activity in the serum and joint fluid of each disease does not show a specific finding about respective disease, but the increased beta-glucuronidase activity was statistically significant in the urine of all disease groups(male:p=0. 0041, female:p=0. 0001). CONCLUSIONS: On the basis of these results, it was suggested that beta-glucuronidase activity was affected by the orthopaedic disease and differed according to each specimen.