The value of TSGF in the changes of TACE before and after treatment in the treatment of primary hepatic carcinoma / 中国医师杂志

Objective To investigate the specific growth factor tumor (TSGF) in the treatment of chemoembolization (transcatheter) in the treatment of primary hepatic carcinoma (HCC) with the change of the level of arterial and transcatheter arterial chemoembolization (TACE),and the evaluation of the value in the treatment.Methods From January 2012 to December 2014 in Inner Mongolia Medical University affiliated Hospital,75 patients with primary liver cancer were treated by TACE,and all patients were treated with TACE.Then the changes of liver function,blood routine,alpha-fetoprotein (AFP),and TSGF levels were monitored at the preoperative,postoperative 1 week,1 month,and 3 months.The curative effect was evaluated.Results At 1 week after operation,aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were significantly higher than those before operation,but no differences were compared to the preoperative 1 month after operation.There was no significant difference in the level of WBC before and after operation.At 1 week after operation,the averaged AFP and TSGF water were significantly increased,but the 1 week after operation was significantly decreased.At 1 month after operation,there was no difference in the levels of TSGF and AFP between complete remission + partial remission + stable disease (CR + PR + SD) and progress disease (PD),and the levels of AFP and CR + PR + SD in PD patients were significantly lower than those in patients with TSGF at 3 months after operation.The levels of TSGF and AFP were lower in PD patients after 3 months,and there was no difference between TSGF and AFP levels at the different time after treatment in patients with CR + PR + SD.There was no significant difference in AFP levels before and after AFP treatment,and the average water level of TSGF before treatment was significantly higher than that of TACE before and after treatment.The difference was statistically significant (P ＜ 0.05).Conclusions TSGF level in patients with primary liver cancer patients with TACE treatment increased after the first rise,especially in the treatment of 1 month after the deterioration of the situation and the tumor has a significant correlation.In the evaluation of curative effect,it has a high value.Combined with AFP,CT examination can increase the long-term efficacy of TACE treatment of primary liver cancer.

The Effect of Platelet-Rich Plasma on Survival of the Composite Graft and the Proper Time of Injection in a Rabbit Ear Composite Graft Model

BACKGROUND: Administration of growth factors has been associated with increased viability of composite grafts greater than 1-cm in diameter. Platelet-rich plasma (PRP) contains many of the growth factors studied. In this study, we evaluate the effect of PRP injection on composite graft viability and the proper time for injection. METHODS: A total of 24 New Zealand White rabbits were divided into four groups. Autologous PRP was injected into the recipient sites three days before grafting in group 1, on the day of grafting in group 2, and three days after grafting in group 3. Group 4 served as control without PRP administration. Auricular composite grafts of 3-cm diameter were harvested and grafted back into place after being rotated 180 degrees. Median graft viability and microvessel density were evaluated at day 21 of graft via macroscopic photographs and immunofluorescent staining, respectively. RESULTS: The median graft survival rate was 97.8% in group 1, 69.2% in group 2, 55.7% in group 3, and 40.8% in the control group. The median vessel counts were 34 (per x200 HPF) in group 1, 24.5 in group 2, 19.5 in group 3, and 10.5 in the control group. CONCLUSIONS: This study demonstrates that PRP administration is associated with increased composite graft viability. All experimental groups showed a significantly higher survival rate and microvessel density, compared with the control group. Pre-administration of PRP was followed by the highest graft survival rate and revascularization. PRP treatments are minimally invasive, fast, easily applicable, and inexpensive, and offer a potential clinical pathway to larger composite grafts.

The expression and significance of connective tissue growth factor after rat corneal alkali burn / 中国医师杂志

Objective To evaluate the effects of connective tissue growth factor (CTGF) on corneal neovascularization.Methods Twenty-five Wistar rats were divided into control and experimental groups.Corneal neovascularization (CNV) was induced by alkaline burn of the cornea with 1 mmol/L NaOH.On the 1st,4th,7th,and 14th day,CNV was observed,and the expression of CTGF was investigated with immunohistochemical method in rat cornea at the different time point.Results On the 4th day,7th day and 14th day after alkaline burn,the areas of CNV were (12.740 ±2.536) mm2,(26.068 ± 10.028) mm2,and (37.588 ± 8.066) mm2,respectively.CTGF was rarely expressed in the cornea of normal rats,and then CTFG expression was quickly increased after alkaline burn,reached the highest level on the 4th day (1.714 ± 0.185),and then declined remarkably on the 7th day (1.334 ± 0.198).Conclusions CTGF might be involved in the formation process of corneal neovascularization after corneal alkali burn.

Yield per plant and other characteristics of soybean plants treated with kinetin and potassium nitrate / Produção por planta e outras características de plantas de soja tratadas com cinetina e nitrato de potássio

The study aimed to evaluate the effect of kinetin and potassium nitrate applied to leaves, at the R3 phenological phase, on some agronomic characteristics of soybean plants and their yield per plant in the field. The experiment was carried out in Itutinga, MG, using five kinetin concentrations (0, 250, 500, 750 and 1.000 mg kinetin ha-1) combined with four potassium nitrate concentrations (0, 5, 10 and 15 kg ha-1) in a factorial scheme using a randomized block experimental design, with three replications. The positioning of numbers of pods per plant was evaluated in three positions in the canopy: lower, middle and upper. The application of potassium nitrate did not influence the studied characteristics. Kinetin increased the total set pod number by 27.4 percent in contrast to the control, mainly in the lower and middle thirds of the canopy. The kinetin increased the pod set number up to 20.7 percent, in the middle third. The yield per plant was increased up to 27.1 percent using the highest kinetin concentration. The increases were attributed to an increase in the endogenous cytokinin levels that contribute to the higher production and better redistribution of assimilates to the pods and seeds.

Objetivou-se, neste trabalho, avaliar o efeito de diferentes doses do regulador vegetal do grupo das citocininas, cinetina, e de nitrato de potássio aplicados via foliar na fase fenológica R3 (início da frutificação) na produtividade e características agronômicas da soja em condições de campo. O experimento foi conduzido em Itutinga, MG, utilizando-se 0, 250, 500, 750 e 1.000 mg de cinetina ha-1 combinadas com as doses de 0, 5, 10 e 15 kg de nitrato de potássio ha-1 em esquema fatorial, num delineamento de blocos casualizados, com três repetições. Avaliou-se o posicionamento dos legumes nas plantas em três posições do dossel, dividido em inferior, médio e superior. A aplicação de nitrato de potássio não influenciou nenhuma das características avaliadas. A cinetina aumentou o número total de legumes fixados em até 27,4 por cento, principalmente, os do terço inferior e mediano do dossel. Observaram-se aumentos dos legumes no terço médio de até 20,7 por cento, perante a testemunha. A produção por planta foi incrementada em até 27,1 por cento, utilizando a maior dose. Atribui-se a causa dos efeitos da cinetina ao aumento dos níveis endógenos de citocininas que contribuíram para uma maior produção e melhor redistribuição de fotoassimilados para os legumes e sementes.

Chronic wound: Pathogenesis and current treatments / 第二军医大学学报

Chronic wound has very complicated pathogenesis and is difficult to treat; the situation has been improving owing to the progress in wound healing research and the clinical application of new techniques. This article focuses on the pathogenesis of chronic wound and introduces progress in various kinds of treatment, such as skin substitute, growth factors, negative pressure treatment, etc.

Effect of oral contraceptive with and without associated estriol on ultrasound measurements of breast fibroadenoma: randomized clinical trial

CONTEXT AND OBJECTIVE: Fibroadenomas are the most common benign tumors of the female breast. The aim of this study was to evaluate the proliferative activity of breast fibroadenoma as shown by ultrasound measurements, following administration of oral contraceptives with and without associated estriol. DESIGN AND SETTING: This was a randomized, double-blind, placebo-controlled clinical trial carried out in the Mastology Sector, Department of Gynecology, Universidade Federal de São Paulo. METHODS: We studied 33 women with fibroadenomas. Ten were placed in group 1 and took an oral contraceptive consisting of levonorgestrel and ethinyl estradiol together with placebo material in the same capsule, for four consecutive cycles with a seven-day interval between them. The other 23 patients constituted group 2 and took the oral contraceptive as above together with estriol in the same capsule, in the same way as done by the group 1 patients. We took ultrasound measurements of their tumors (in three dimensions) before and after the intake of medication. At the end of the study, all the patients had their tumors removed by surgery. RESULTS: We observed decreased fibroadenoma width among the users of oral contraceptives with placebo, and this decrease was statistically significant. In the other group, we did not observe any changes (in width, length or height). CONCLUSION: The results confirm that estriol may block the protective effect of oral contraceptives on fibroadenomas, since we observed decreased fibroadenoma width among the group 1 patients but not the group 2 patients.

CONTEXTO E OBJETIVO: Fibroadenomas são os tumores benignos mais comuns na mama feminina. Avaliamos a atividade proliferativa do fibroadenoma mamário por medidas ultra-sonográficas após a administração de anticoncepcional hormonal combinado oral, associado ou não ao estriol. TIPO DE ESTUDO E LOCAL: Ensaio clínico randomizado, duplo-cego, placebo-controlado, realizado na Universidade Federal de São Paulo. MÉTODOS: Foram estudadas 33 pacientes portadoras de fibroadenoma, do setor de Mastologia da Disciplina de Ginecologia da Universidade Federal de São Paulo, sendo que 10 mulheres constituíram o grupo 1 e utilizaram anticoncepcional oral composto de levonorgestrel e etinilestradiol, associados a um comprimido de placebo, na mesma cápsula, por quatro ciclos consecutivos, com intervalo de sete dias entre os mesmos. As restantes 23 pacientes alocaram-se no grupo 2 e ingeriram, além do anticoncepcional oral descrito acima, um comprimido de estriol, que foi manufaturado conjuntamente ao anticoncepcional, em uma mesma cápsula, sendo utilizado da mesma forma que nas pacientes do grupo 1. Realizamos medidas ultra-sonográficas dos tumores (três dimensões) antes e após a ingestão da medicação. Ao término do estudo, as pacientes sofreram exérese de suas tumorações. RESULTADOS: Obtivemos diminuição da largura nos fibroadenomas de pacientes usuárias apenas de anticoncepcional oral e esse resultado foi estatisticamente significante. Não houve alteração de nenhuma dimensão (largura, altura ou comprimento) no outro grupo. CONCLUSÕES: Os resultados corroboraram que o estriol bloquearia o efeito protetor do anticoncepcional hormonal combinado oral sobre os fibroadenomas, já que observamos diminuição na largura dos fibroadenomas das pacientes do grupo 1 e não do grupo 2.

Investigation of KGF and KGFR expression in external auditory canal skin adjacent to tympanic membrane perforation / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the continuous expression of keratinocyte growth factor(KGF)and keratinocyte growth factor receptor(KGFR)in external auditory canal skin adjacent to tympanic membrane perforation and analyze the role of KGF and KGFR in the different turnover of chronic otitis media.METHODS The external auditory canal skin adjacent to tympanic membrane perforation from 20 cases with cholesteatoma otitis media and the corresponding cholesteatoma tissue and normal external ear skin were examined by immunohistochemical S-P method and quantitative analysis.The positive rate was compared with 20 cases of non-cholesteatom otitis media's external auditory canal skin adjacent to tympanic membrane perforation.RESULTS The staining for KGF and KGFR in cholesteatoma otitis media's external auditory canal skin adjacent to tympanic membrane perforation was consistently stronger than that in non-cholesteatoma otitis media.The positive rates of the two tissue was(33.135?6.364)% and(19.965?10.570)%,(19.380?2.827)% and(13.145?7.935)% respectively,revealing a significant difference.CONCLUSION The activity of hyperproliferation of the external auditory canal skin adjacent to tympanic membrane perforation in cholesteatoma otitis media is stronger than that of non-cholesteatoma otitis media.KGF and KGFR may play a more important role for hyperproliferation of cholesteatoma.

The expression and significance of keratinocyte growth factor in the regulation of cholesteatoma growth / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the expression of keratinocyte growth factor(KGF)in middle ear cholesteatoma and to explore the role of KGF on the hyperproliferation of the cholesteatoma epithelium and the formation and development of cholesteatoma. METHODS The specimens from the cholesteatoma tissue of 20 cases and the corresponding normal external ear skin were examined by immunohistochemical S-P method and quantitative analysis. RESULTS In normal ear skin only stroma staining for KGF was positive. In cholesteatoma epithelium staining for KGF was strongly positive and the stroma staining was stronger than that of normal ear skin. The positive rates of the cholesteatoma and normal external ear skin revealed a significant difference. There was a positive correlation between cholesteatoma epithelium staining for KGF and for Ki67 and the coefflcient of correlation was 0.609(P﹤0.01). CONCLUSION There was correlation between the expression of KGF or Ki67 and the ability of reproduction of middle ear cholesteatoma. Local inflammation might promote hyperproliferation of the epithelium of cholesteatoma by regulating the expression of KGF. It suggested that an autocrine stimulation of KGF correlate with the occurrence and development of cholesteatoma .

Comparison of Bone Marrow Stromal Cells with Fibroblasts in Wound Healing Accelerating Growth Factor Secretion

Cryopreserved fibroblast implants represent a major advancement for healing of chronic wounds. Bone marrow stromal cells, which include the mesenchymal stem cells, have a low immunity-assisted rejection and are capable of expanding profoundly in a culture media. Therefore, they have several advantages over fibroblasts in clinical use. The ultimate goal of this study was to compare the wound healing accelerating growth factor secretion of the bone marrow stromal cells with that of the fibroblasts and this pilot study particularly focuses on the growth factor secretion to accelerate wound healing. Bone marrow stromal cells and fibroblasts were isolated from the same patients and grown in culture. At 1, 3, and 5 days post-incubating, secretion of basic fibroblast growth factor(bFGF), vascular endothelial growth factor (VEGF), and transforming growth factor beta(TGF-beta) were compared. In TGF-beta secretion fibroblasts showed 12~21% superior results than bone marrow stromal cells. In contrast, bFGF levels in the bone marrow stromal cells were 47~89% greater than that in fibroblasts. The VEGF levels of the bone marrow stromal cells was 7~12 fold greater than that of the fibroblasts. Our results suggest that the bone marrow stromal cells have great potential for wound healing accelerating growth factor secretion.

Hypoxia induces the expression and secretion of connective tissue growth factor and fibronectin by cultured renal cortical myofibroblasts / 北京大学学报(医学版)

Objective: To investigate whether hypoxia can affect the expression and secretion of connective tissue growth factor(CTGF) and fibronectin(FN) in primary cultured rat renal cortical myofibroblasts . Methods: The primary cultured rat renal cortical myofibroblasts were subjected to hypoxic (1%O_2) or normoxic (21% O_2) conditions for a variety of times. The protein levels of HIF - 1?, CTGF and FN protein were analyzed by Western blotting in both the whole cell lysates and supernatant culture medium 6 h,12 h and 24 h after incubation, respectively. RT-PCR was carried out to measure the levels of FN mRNA at different time points (2 h,3 h,6 h and 12 h). The activity of gelatinase MMP-2 and MMP-9 in the supernatant from the cultured cell medium was assayed by gelatin zymography. Results: The expression of HIF - 1?was induced at h6 in cells under hypoxia incubation. The levels of cellular CTGF protein were increased in hypoxia treated myofibroblasts at h6 (175%?52%),significantly elevated at h12 (347%?67%,P

The co-stimulatory effect of growth factors on chondrogenesis induction of rat mesenchymai stem cells / 中华风湿病学杂志

Objective The present study aims to explore the optimal combination of growth factors, including TGF-?_1,IGF-I and BMP-2,for the induction of ehondrogenesis of MSCs in vitro monolayer culture. Methods MSCs were induced to differentiate into ehondrocytes in the medium with the following growth fac- tors:TGF-?_1,BMP-2 and IGF-I,either alone or in combination.We detected two cartilage specific markers in differentiated cells.Alcian blue stain was used for aggreean and RT-PCR for collagen type II mRNA.Results The level of Collagen typeⅡmRNA of group BMP-2+IGF-I,TGF-?_1,TGF-?_1+IGF-I,TGF-?_1+BMP-2 and TGF-?_1+IGF-I+BMP-2 was 0.147?0.062,0.478?0.069,0.489?0.022,0.653?0.013,0.807?0.051 respectively. Alcian blue stain in all gruups were positive.Conclusion The TGF-?_1+IGF-I+BMP-2 group is the most ef- fective combination in chondrogenesis induction.

The Effect and Significance of Intestinal Trefoil Factor IL-8 and MDA for Neonatal Rat Model for Hypoxia-induced Intestinal Injury / 中华围产医学杂志

Objective To explore the effects of intestinal trefoil factor (ITF) on interleukin-8(IL-8) and malondiadehyde (MDA) of neonatalrats' intestinal tissue post necrotizing enterocolitis(NEC), to investigate whether ITF has some protective effects on NEC.. Methods Thirty-two neonatal tats of NEC model were devided into four groups, (1)NEC+FTF 0.5 mg, (2) NEC+ITF 0.2 mg, (3) NEC, and (4) all others. After NEC model of neonatal rats was established and the ITF was treated, all the neonatal rats were returned to their mothers. In the 4th day, all the subjects were put to death. Intestinal tissue located at the boundary of ileum and cecum was obtained to observe hostological changes. Other intestinal sissue was removed to homogenate. Than the homogenate was centrifuged, supernates were used to determine the content of IL-8 and MDA. Results Content of IL-8 was dramatically less in group A and B than group C, respectively (29.722?7.134)、(30.512? 8.230)、(39.379 5?4.420) [pg/(mg?pro)](P0.05). Content of MDA was dramatically less in group A and B than group C, respectively(2.267?0.267)、(2.154?0.301)、(3.378?0.835)[nmol/(mg?pro)](P

Study on the Serum Levels of Placenta Growth Factor During Normal Pregnancy and in Patients with Pregnancy Induced Hypertension / 吉林大学学报(医学版)

Objective:To detect serum levels of placenta growth factor(PIGF)throughout normal pregnancy and in patients with pregnancy-induced hypertension(PIH)as well as to explore the relationship between P1GF and pathogenesis of PIH. Methods: Serum specimens were collected from 51 healthy pregnant women as a control group and 33 women suffered from PIH as third trimester of normal pregnancy(P＜0.001).There was a trend that serum levels of P1GF in PIH group decreased with the severity of PIH(P＜0.001).Conclusion:PlGF play important roles in placental angiogenesis throughout pregnancy, and decreased serum levels of PlGF is associated with PIH.

Short-term Morphological and Growth Factor Changes in Rat Bladders Augmented with a Porcine Small Intestinal Submucosa / 대한비뇨기과학회지

PURPOSE: This study was performed to investigate the short-term changes in the morphology and growth factors after the augment of rat bladders with a porcine small intestinal submucosa (SIS). MATERIALS AND METHODS: The experiments were performed in 39 female Sprague- Dawley rats, comprised of 3 controls, 18 sham-operated and 18 SIS-augmented rats. The sham-operated group underwent a primary closure after a longitudinal incision of the bladder. The augmentation cystoplasty was performed using a porcine SIS after a hemi-cystectomy. The rats were postoperatively sacrificed at periods ranging between 12 hours and 14 days, and their bladders procured for histological examination. Reverse transcription-polymerase chain reactions (RT-PCR) were performed to evaluate the expressions of the keratinocyte growth factor (KGF) and the transforming growth factor-alpha (TGF-alpha). RESULTS: Both groups showed an acute inflammatory reaction during the immediate postoperative period, and chronic inflammatory cells appeared 48 hours postoperatively, with new capillaries and fibroblasts appearing 4 days postoperatively. The disrupted urothelial barrier was restored on the 7th postoperative day in the sham group, and epithelialization began to appear on day 4 in the augmented group. New granulation tissue covered the small intestinal submucosa, like a sheet of membrane, by the 4th postoperative day, and its thickness and cellularity increased with time. The mRNA expressions of the KGF and TGF-alpha increased during the initial postoperative period, but tended to derease with time. Their expressions coincided with acute inflammatory reactions. CONCLUSIONS: In conclusion, the short-term morphological changes after the augmentation cystoplasty, with a porcine small intestinal submucosa, were initially composed of acute inflammation, chronic inflammation, fibroblast aggregation, neoangiogenesis, epithelialization and formation of granulation tissue. The mRNA expressions of the KGF and TGF-alpha coincided with acute inflammatory reactions.

Interstitium revascularization and related cytokine regulation in myocardium of rheumatic heart disease / 中华风湿病学杂志

Objective To investigate the interstitial revascularization and related cytokine regulation in myocardium of rheumatic heart disease.Methods The specimens were stained by HE to examine the ratios between arteriolar inner and outer diameters and by immunohistochemical staining and in situ hybridization to measure the expression of bFGF,vascular endothelial growth factor (VEGF),? SMA and mRNA of bFGF.Then the relationship between ratios of arteriolar inner and outer diameters and expression of bFGF,VEGF and ? SMA.Results The imaging analysis indicated that there was significant myocardium fibrosis,and the arteriolar wall became thick (0 68?0 15 vs 0 40?0 04).The expression of bFGF,VEGF and ? SMA in the myocardium of rheumatic heart disease was significantly higher than that of healthy adult control group.There was a positive relationship between ratios of arteriolar inner and outer diameters and expression of bFGF,VEGF and ? SMA (bFGF r =0 719, P

The correlation among connective tissue growth factor,transforming growth factor-? receptor,and extracellular matrix in human proliferative membranes of proliferative vitreoretinopathy / 中华眼底病杂志

0.05).Statistical analysis showed that there was a correlation between the expression of CTGF and TGF-?RⅡ,FN,and collagen Ⅰ and Ⅲ protein,respectively. Conclusions The expression of CTGF and TGF-?RⅡ protein is up-regulated in PRM of PVR,which suggests that the activation of TGF-?RⅡ is involved in the production of CTGF,and CTGF is closely related to the production of ECM and play an important role in the pathogenesis of PVR.

Studies on the effects of different growth factors to perfused rabbit kidney in vitro / 解放军医学杂志

Objective To investigate the effects of different growth factors to perfused kidney in vitro at room temperature. Methods 25 New Zealand rabbits were randomly divided into 5 groups, Each group had 5 rabbits. Perfusion was carried out in vitro on rabbits′ kidney with different culture media containing hepatocellular growth factor(HGF) in high or low dosage, epidermal growth factor(EGF) in high or low dosage, and growth factors-free. On the 65th hour after perfusion, lactate dehydrogenase(LDH) activity, the concentration of sodium and potassium in kidney vein effluent and ?-N-acetyl amino glucosaminidase in urine (NAG) activity in each group were respectively detected to observe the renal function. Renal histological changes under light microscope and proliferating cell nuclear antigen (PCNA) of renal tissue were also examined to observe the renal morphological changes and the lesion degrees of renal tissue. Results The preservation effect of the culture media containing HGF in high dosage was the best among all 5 groups. In this group, LDH activity, the concentration of potassium in kidney vein effluent and NAG activity in urine were significantly lower than that of other 4 groups; the concentration of sodium of kidney vein effluent was notably higher than any other group. Renal histological changes under light microscope and PCNA stain of renal tissue showed that the renal morphological damage and the lesion of renal tissue were lighter than any other group. Conclusion HGF in high dosage could markedly improve the effect of perfusion preservation and protect the function and morphology structure of perfused rabbits′ kidneys better.

The relationship between the urine albumin excretion rate, the growth factors and bone density in elderly male patients with type 2 diabetes mellitus / 中华老年医学杂志

Objective To observe the relationship between urine albumin excretion rate (UAE), growth factors and the bone mineral density (BMD) in elderly patients with type 2 diabetes mellitus. Methods Fourteen controls and 51 elderly male patients with diabetes divided into three groups: normal albuminuria(UAE

Effects of 4 sorts of growth factors on the cell growth of androgen independent prostate cancer cell line PC-3M / 中华老年医学杂志

Objective To evaluate the effects of TGF ? 1, EGF, bFGF, KGF on the cell growth of androgen independent prostate cancer cell line PC 3M in vitro. Methods We cultured androgen independent prostate cancer cell line PC 3M in vitro and added TGF ? 1, EGF, bFGF, KGF to the medium at different concentrations of 1, 5,10 and 50 ng/ml. Brdu incorporation method was used to determine the status of cell proliferation. Results The absorbance ( A ) value of the control group PC 3M was 0 759. Seventy two hours after adding the growth factors separately, the inhibition was enhanced with the increasing of concentrations. At the dosage of 50 ng/ml, A value decreased to 0 400(TGF ? 1),0 455(EGF),0 532(bFGF),and 0 491(KGF),respectively, showing significant difference compared to the controls. When the mixture of 1 ng/ml EGF with 0 5 ng/ml or 5 ng/ml TGF ? 1 was added into the medium, A values were 0 522 and 0 299, respectively. Conclusions All these four growth factors inhibited the proliferation of PC 3M cell line in a dose dependent manner. TGF ? 1 and EGF have no interaction to the cell proliferation.

Dynamic changes of enzymes activities and growth factors contents in human colostrum / 中华妇产科杂志

Objective To study the dynamic changes of the activities of superoxide dismutase (SOD), amylase (AM) and alkaline phosphatase (ALP) and of the contents of epithelium growth factors (EGF) and insulin growth factors (IGF-1) in human colostrum (lactation 1-7 days postpartum). Methods A total of 118 samples of human colostrum were taken from 20 healthy women postpartum. The activities of SOD, AM and ALP in human colostrum were determined by methods of pyrogallol autoxidation, colorimetry of iodine-starch and pyrocatechol phosphate respectively. EGF and IGF-1 contents in human colostrum were measured by radioimmunoassay. Results The SOD activity of human colostrum increased slightly during lactation 1-4 days postpartum, from (18.7?2.2) kU/L to (22.5?2.9) kU/L, then it tended to decrease, being (11.2?2.1) kU/L on the 7th day. The SOD activities in lactation 1-5 days postpartum were significantly higher than that on day 7 ( P