ABSTRACT
For the first time a simple, rapid and accurate stability indicating HPLC method is described for simultaneous quantification of atenolol and nifedipine in bulk powder and dosage form. Chromatographic separation was carried out on Intersil® reversed phase C18 column. Separation was done using gradient binary mobile phase of ACN and 50 mM NaClO4 in the ratio from 5: 95 to 50: 50 (v/v) within 8 minutes at flow rate of 1 mL/min and 30 °C. An UV detector was used at 230 nm for detection. The elution times of atenolol and nifedipine were found to be 6.05±0.02 and 14.50±0.04 minutes, respectively. The method was validated for system suitability, linearity, precision, limits of detection and quantitation, specificity, stability and robustness. Robustness study was done for small changes in temperature, flow rate, wavelength of detection and time to reach 50% of ACN in mobile phase. Stability tests were done through exposure of the analytes' solution for five different stress conditions. The limit of detection for both drugs was 0.04 μg mL-1. Limits of quantitation were found to be 0.12 μg mL-1 for atenolol and 0.11μg mL-1 for nifedipine. The recovery value of this method was 100.40±0.85% for atenolol and 100.30±1.10% for nifedipine.
ABSTRACT
Objective: A reversed-phase HPLC method was established for the simultaneous determination of five hydrophilic and lipophilic components in the roots of Salvia miltiorrhiza. Methods: Hydrophilic components including danshensu, protocatechuic aldehyde, and salvianolic acid B, and lipophilic components such as cryptotanshinone and tanshinone IIA, were successfully separated on a Waters Symmetry C18 reverse-phase column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.5% phosphoric acid (gradient elution) as mobile phase, the detection wavelength was set at 281 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 30 °C. Results: The recovery of the method was in the range of 95.1%-102.5% and the precision was less than 3% for all five analytes. All the compounds showed good linearity (R2 > 0.9990) in a relatively wide concentration range. Therefore, this HPLC method demonstrated good reproducibility, stability, and accuracy in validation studies. Conclusions: Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation on the roots of S. miltiorrhiza.
ABSTRACT
A method to determine dihydroxyacetone (DHA) in fermentation broth was developed by high performance liquid chromatography (HPLC). DHA was separated on a Alltima C18(5?m,250?4.6mm). The mobile phase was 0.5% methanol solution (pH adjusted to 3.0 with H3PO4), the flow-rate was 1.0 ml/min and the detective wavelength was 200 nm. The detection limits of DHA was 0.1 g/L～10.0 g/L. 6.2 g/L DHA in the fermentation broth was detected by HPLC, which was in agreement with the result by spectrophotometric method.The method was applicable for DHA determination in the fermentation process.
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Objective: To establish the quality control standards for Brains' King Oral solution. Methods: Rhizoma Gastrodia, Rhizoma et Radix Noto-pterygii in oral solution were identified by TLC. The content of gastrodin in Brain's King Oral Solution was determined by HPLC with external standard. The average recovery was 99.22% and RSD was 0.99%(n=6).Results: These methods are simple, accurate and specific.Conclusion: These methods can be used for the quality control of Brain's King Oral Solution.
ABSTRACT
Reversed high-pressure liquid chromatography was applied in content determination of bufogenin in Liuying Pills. The sample was extracted with chloroform. Using the calibration curve method of external peak area for quantitation, ODS as chromatographic column, 60％ methanol as mobile phase, and detection wavelength at 298nm, the recovery reached 97.7％(CV=0.5％).
ABSTRACT
The content of schizandrin, A and B in Fructus Schisandrae and its preparation——Gengnianan has been determined by HPLC on YWG C_(18) column. Mobile phase consists of acetonitrile and water (70:50). Detection is at 254nm with the UV detector.
ABSTRACT
The reversed-phase high-performance chromatography was developed for determing the content of berberine, palmatine, emodin and chlorogenic acid in Xiaoerniuhuang Powder on ODS column. The powder samples extracted with methanol were determined separatly at their particular absorption points using external standard method.This method had advantages of perfect separatin, high sensitivity, good reproducibility and simple operation.