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OBJECTIVE: To establish an HPLC method for determination of the related substances of ambrisentan tablets. METHODS: Gradient elution was performed on an Agela Venusil MP C18 column (4.6 mm × 250 mm, 5 μm). The mixture of 0.02 mol · L-1 phosphate buffer (pH 3.0) and acetonitrile (62:38) was used as mobile phase A, and acetonitrile as mobile phase B. The flow rate was 1.0 mL · min-1; the detection wavelength was set at 220 nm; and the column temperature was maintained at 35℃. RESULTS: After being treated with acid, base, heat, and oxidation, ambrisentan underwent more or less degradation. The method could effectively detect the degradation products of ambrisentan; the separation of impurities was good; the method had good specificity, linearity, and durability. CONCLUSION: The proposed method can be used to separate and determine the related substances of ambrisentan tablets, and is suitable for the quality control of this drug.
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OBJECTIVE: To develop a method for separating and determining the related substances in carbazochrome sodium sulfonate injection. METHODS: HPLC Gradient elution was performed on an HP-ODS Hypersil colum. The mixture of 0.01 mol·L-1 phosphate buffer (pH 3.0) and acetonitrile (94:6) was used as mobile phase A, and acetonitrile as mobile phase B. The flow rate was 1.0 mL·min-1, the detection wavelength was set at 220 nm. RESULTS: After being treated with acid, base, heat and oxidation, carbazochrome sodium sulfonate underwent more or less degradation, the degradation products could be detected by the proposed method. Among the practical carbazochrome sodium sulfonate injection and carbazochrome sodium sulfonate and sodium choloride injection samples, the thermo degradation impurities but no impurities resulted from other treatments were detected in carbazochrome sodium sulfonate injections and carbazochrome sodium sulfonate sodium chloride injections. CONCLUSION: The proposed method can be used to separate and determine the related substances in carbazochrome sodium sulfonate injection, and is suitable for the quality control of this drug.
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OBJECTIVE: To establish an HPLC method with gradient elution for the determination of resorcinol and triamcinolone acetonide acetate in compound resorcinol cream. METHODS: The HPLC was conducted on a ZORBAX Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm) with acetonitrile (A)-0.5% ammonium acetate solution (B) as the mobile phase. The gradient elution program was as follows: 0-3 min (25% A), 3-5 min (25% A-55% A), and 5-15 min (55% A). The column temperature was 40°C. The flow rate was 1.0 mL · min-1 and the detection wavelength was 240 nm. RESULTS: The linear ranges of resorcinol and triamcinolone acetonide acetate were 103.18-515.90 μmg · mL-1 (r=1.0000) and 1.336-6.680 μg · mL-1 (r=0.999 4), respectively. The average recoveries were 99.3% (RSD=0.62%) and 99.1% (RSD=0.73%), respectively. CONCLUSION: The proposed method is simple, rapid and accurate. It has been applied in the assay of samples with satisfactory results. Copyright 2012 by the Chinese Pharmaceutical Association.
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Objective: A new HPLC gradient elution was studied for the determination of notoginsenoside R 1, ginsenoside Rg 1 and Rb 1 in Xuesaitong Dripping Pills. Methods:C 18 column was used with a mobile phase of acetonitrile water 0～15 min. of (20∶80～40∶60) gradient elution, post time was 5 min, the wavelength of detecter was set at 203nm. Results: The linear range of R 1, Rg 1 and Rb 1 was 1.02～9.18?g,4.8～ 43.4?g and 4.6～41.6?g, respectively, the average recovery rate was 101.0% ( RSD =3.18%), 99.5%( RSD =3.02%) and 100.0%( RSD =1.19), respectively. Conclusion: The method is rapid, accurate, sensitive and relieble. The results show that the method can be used to control quality of products.
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Objective: To monitor the quality and production procedure of Yiwei Capsules from various aspects. Methods: The method of HPLC gradient elution analysis was adopted to determine paeoniflorin, hesperidin, and glycyrrhizic acid of Yiwei Capsules at the same time. Results: Good results were obtained in recoveries and coefficient of variation of the three substances. Conclusion: In this method the pretreatment is simple and the operation is easy which is helpful in quick and accurate determination.
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AIM:A HPLC linear gradient elution method was studied for determining notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 content in Xinnao guantong Guttate Pill.METHODS:The YMC-Pack ODS-A column was used with a mobile phase of acetonitrile-water 0-15 min(23∶77),16-40 min(50∶50)gradient elution,the wavelength of detecter was set at 203 nm.RESULTS:The linear ranges of R_1,Rg_1,Rb_1 were 0.204-1.836,0.818-7.362,0.858-7.722 ?g,respectively.The average recoveries of R_1,Rg_1,Rb_1 were 97.86%,101.71%,101.44%,respectively.CONCLUSION:The method is rapid,accurate,sensitive and reliable.The results show that method can be used to control quality of products.
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?-carotene of vegetables was extracted with petroleum ether and ether (1:1). The extract was evaporated at 45℃ in water bath. The residue was dissolved in n-hexane and analysed on a column of?Bondapak C18 with gradient elution, methyl alcohol as mobile phase. It was then detected at 450nm by spectrophotometer. The retention time of ?-carotene was 13.71min. The recoveries were 94.1% to 102.2%. The coefficient of variation was 2.38% to 5.17%. The method was simple and rapid.