ABSTRACT
Objective To compare the chemical composition and the pharmaceutical effect of anti-heart failure of wild commodity Astragali Radix (WAR) and cultivated Astragali Radix (CAR). Methods The contents of flavonoids and saponins in Astragali Radix samples were determined by HPLC-UV-ELSD. The intervention effects of wild commodity and cultivated Astragali Radix on traditional efficacy index and metabolomics were compared in rat model with heart failure induced by adriamycin. Results The contents of total flavonoids, saponin I, and saponin III in WAR were significantly higher than those in CAR, but the content of total saponins was the equivalent. Pharmacodynamics experimental results showed that both WAR and CAR could improve the general situation in rats, histopathology, cardiac function parameters, serum biochemical indicators, and BNP. However, compared with the model group, the intervention effects of the WAR on cardiac function parameters (EF, FS, and LVIDs), biochemical markers (CK) and serum BNP content were superior to the CAR, and showed significant differences. The principal component analysis showed that the metabolic profiles of the normal control group and the model group were clearly distinguished. A total of 14 potential biomarkers associated with heart failure were identified, which could be adjusted to varying degrees in WAR and CAR, of which 2-hydroxyisobutyric acid, acetone, pyruvate, and formate were significantly changed in the WAR group. Conclusion In this study, the intervention of WAR in heart failure rats was significantly better than that of CAR, which provided a scientific basis for the establishment of specification grading, the clinical rational drugs use, and the classification of Astragali Radix by pharmaceutical companies for Astragali Radix.
ABSTRACT
Objective: To develop an HPLC-UV-ELSD method for the simultaneous determination of crenelatin, gallic acid, salidroside, tyrosol, harpagide, harpagoside, angoroside C, and cinnamic acid in Compound Rhodiola Capsule. Methods: Kromasil C18 column (250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was composed of acetonitrile (A) and 0.3% HAC (B) with gradient elution. The flow rate was 1.0 mL/min and the detection wavelength was 275 nm. the column temperature was 30℃, and the evaporative light-scattering detector (ELSD) drift tube temperature was 40℃, and gas pressure of 1.5 bar (150 kPa). Results: Crenelatin, gallic acid, salidroside, tyrosol, harpagide, harpagoside, angoroside C, and cinnamic acid were separated well. The linear calibration curves were obtained in 67.084-670.84 μg/mL for crenelatin, R2=0.9992; 11.410-114.100 μg/mL for gallic acid, R2=0.9994; 78.995-789.95 μg/mL for salidroside, R2=0.9996; 19.625-196.25 μg/mL for tyrosol, R2=0.9997; 59.368-593.68 μg/mL for harpagide, R2=0.9998; 62.585-625.85 μg/mL for harpagoside, R2=0.9995; 55.045-550.45 μg/mL for angoroside C, R2=0.9996; and 6.895-68.95 μg/mL for cinnamic acid, R2=0.9998. The average recoveries of the eight constituents were 100.8%, 98.9%, 100.1%, 100.8%, 98.9%, 99.6%, 100.7%, and 99.2% with RSD of 0.64%, 0.56%, 0.35%, 0.65%, 0.26%, 0.58%, 1.00%, and 0.64%. Conclusion: The method is convenient, accurate, and can be used for the simultaneous determination of the preparation.
ABSTRACT
A HPLC-UV-ELSD method was established for simultaneous determination of six components in two intermediates of Shenqi Fuzheng injection (SFI) and the feasibility of establishing quantitative analysis of multi-components by single marker (QAMS) methods on different detectors was further explored. Calycosin-7-O-β-D-glucoside and astragloside Ⅳ were selected as internal reference substances for respectively flavonoids and saponins, and relative correlation factors (RCF) of formononetin-7-O-β-D-glucoside, 9, 10-dimethoxypterocarpan-3-O-β-D-glucopyranoside, 2'-dihydroxy-3', 4'-dimethoxyisoflavan-7-O-β-D-glucopyranoside and astragloside Ⅱ were calculated. Eventually, quantitative results of the 14 samples were compared between QAMS and external standard method. The sample concentrations calculated by QAMS were similar with concentrations calculated by external standard method, and the absolute values of relative deviations were generally less than 5% according to the UV detection of flavonoids. On the basis of ELSD detection for saponins, however, the absolute values of relative deviation of the two methods ranged from 0.48% to 23.17%. The QAMS method built on ultraviolet (UV) detectors was stable and can be used as a substitute method to reduce the consumption of standard compounds; meanwhile, the accuracy of QAMS method built on evaporative light scattering detector (ELSD) was inferior to that of external standard method, and the working principle of ELSD and feasible concentration range remain to be further studied.