The early renal injury diagnostic value of new markers joint detection for type 2 diabetic kidney injury / 重庆医学

Objective To explore the diagnosis value of urinary molecular markers of Kidney injury molecule 1(Kim-1),heat shock protein 72(Hsp72),plasminogen activator inhibitor 1(PAI-1),neutrophil gelatinase associated lipocalin(NGAL)joint detec-tion in the diagnosis of early type 2 diabetes renal damage.Methods Totally 187 cases of type 2 diabetes mellitus patients were sep-arate into two groups based on combination of four urine proteins[Microalbumin(MA),urinary transferrin(TRU),urine immuno-globulin(IGU),urine alpha 1 microglobulin (A1M)].Group A had 48 cases,and the urine protein of patients were negative.Group B had 139 cases,and the urine protein of patients were positive.The normal control group(NC group)selected 50 cases of urinary protein negative healthy people.MA,TRU,IGU,A1M were detected by rate scattering turbidimetric method,and Kim-1,Hsp72, PAI-1and NGAL were detected by enzyme-linked immunosorbent.Results Compared with NC group,the levels of urine Kim-1, Hsp72 and PAI-1 had significant increase in group A(P 0.5).The levels of Kim-1,Hsp72,PAI-1 and NGAL significantly increased in group B(P <0.01),and the increase value was more significantly in group B(P <0.01).The positive rates l of Kim-1,Hsp72,PAI-1 and NGAL joint detection reached 66.70% in group A,which was significantly higher than single detection(P <0.01 ).Conclusion The levels of Hsp72,PAI-1,Kim-1 NGAL protein have been a significant exception before urinary protein in type 2 diabetes mellitus patients,and the abnormal is obvious with the increase of renal damage degree.Kim-1,Hsp72,PAI-1,NGAL can be used as early biomarkers of kidney injury in type 2 diabe-tes,the joint detection will greatly increase the positive detection rate.

Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats

BACKGROUND: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-dia-betic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats. METHODS: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days. RESULTS: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats. CONCLUSION: This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.

Impact of peptide binding domain of heat shock protein 72 on epithelial to mesenchymal transition / 中华肾脏病杂志

Objective To investigate the effects of peptide-binding domain (PBD) of heat shock protein (HSP) 72 on epithelial to mesenchymal transition (EMT) in rat renal tubular epithelial cells.Methods The expressions of wild-type HSP72,mutant of HSP72 lacking peptide binding domain (HSP72-△PBD) and HSP72-PBD were induced by plasmid transfection.NRK-52E ceils were stimulated by TGF-β1 for 48 h.The expressions of α-smooth muscle actin (α-SMA),E-cadherin,HSP72 and Smad3/p-Smad3 were detected by Western blot and immunofluorescence.Results After NRK-52E cells were stimulated by TGF-β 1 (10 μg/L) for 48 h,the expression of α-SMA was increased and the protein level of E-cadherin was decreased.Western blotting and immunofluorescence showed that over-expression of both HSP72 and PBD inhibited TGF-β1-induced up-regulation of protein α-SMA expression,down-regulation of protein E-cadherin.However,overexpression of HSP72-△PBD did not change the protein level of E-cadherin and α-SMA.In addition,over-expression of HSP72 and PBD significantly inhibited the phosphorylation of Smad3.Conclusion Inhibition of Smad3 activation and EMT by HSP72 is associated with the function of PBD.

Expression of HSP72 in Nasal Mucosa of Patients with Allergic Rhinitis / 대한이비인후과학회지

BACKGROUND AND OBJECTIVES: Heat shock protein (HSP) is to be involved in inflammation. HSP may be expressed in allergic rhinitis which is an inflammation disease. The purpose of this study was to investigate whether overexpression of HSP72 is specific for allergic rhinitis and is correlated with clinical severity of disease. MATERIALS AND METHODS: We studied expression of HSP72 by immunohistochemistry using ABC technique on nasal mucosa from 20 patients with allergic rhinitis, 10 patients with chronic hypertrophic rhinitis and 10 patients with normal turbinates. RESULTS: In biopsies from patients with allergic rhinitis, immunopositive cells were observed on epithelium, basement membrane, submucous gland cells, and inflammatory cells. Expression of HSP72 in patients with allergic rhinitis was significantly increased in nasal mucosa (p<0.01) and was significantly correlated with clinical severity of the disease (p<0.01) and eosinophil count (p<0.01). CONCLUSION: Inflammation of nasal mucosa in patients with allergic rhinitis may be linked to production of HSP72.

Hypothermic preconditioning lowers the incidence of hypothermic arrest in neonatal rat

This study was performed to examine 1) Whether hypothermic cardiac arrest produces myocardial HSP72 expression; 2) And if, whether it serves to protect the heart against the subsequent hypothermic arrest. In the present study, neonatal rats were placed in an icebath to induce hypothermia. To determine whether hypothermic cardiac arrest produces myocardial HSP72, experimental animals were subjected to 10-min hypothermic insult before the extraction of the heart. The intervals between the insult and extraction were 1 (1 HR), 4 (4 HR), 8 (8 HR), 24 (24 HR) or 72 (72HR) hours. A minimal amount of HSP72 was detected in control, 1 HR and 72 HR groups. In contrast, 8 HR and 24 HR groups showed a significant level of HSP72 expressions. To assess the cardioprotective effect of HSP72 against hypothermic cardiac arrest, we compared the proportion of recovery from the arrest between control and preconditioned (PREC) animals. Control animals were subjected to 20-min hypothermic insult, while PREC group was preconditioned by 10-min hypothermic insult 8 hours before the 20-min test hypothermic insult. Resuscitation rate from cardiac arrest induced by the 20-min hypothermic insult in PREC group was significantly higher than that in controls. These results suggest that the cardioprotective effect of hypothermic preconditioning is associated with an increase in HSP72 expression.

The Relationship between Corneal Opacity and Immunohistochemical Expression of Heat Shock Protein 72/73 after Excimer Laser Photorefractive Keratectomy

Excimer laser PRK (photorefractive keratectomy) for myopic correction provides an accurate correction in the cases of moderate myopia with a little side effect, but in high myopia it frequently causes corneal opacity and myopic regression. To determine the etiology of the corneal opacity, the author studied on the immunohistochemical stain for heat shock protein 72/73 after myopic excimer laser PRK. Thirty Newzealand white rabbits (60 eyes) were divided into four groups: A, B, C, and control group. Myopic PRK was carried out on the cornea to correct the amounts of -6D myopia(group A), -9D(group B), and -15D(group C) with multizone technique using Excimer laser(VisX 20/20, Sunnyvale, USA). The corneal status was examined using a slit lamp. The eyes were enucleated at 6, 24, 48hours, 1 week and 8 weeks after operation. The corneas were obtained and sectioned for immunohistochemical stains. Corneal opacity was found with the mean density of 0.83(A group), 1(B group), and 1.67(C group) 8 weeks after the operation. Hsp 72 immunoreactive staining was noted in corneal epithelium in the C group at 24 hours after operation and maximal expression was found at 48 hours after operation. Hsp 73 was seen in corneal epithelium and stroma of all group. But maximal expression was observed in B and C groups 48 hours after operation. Consquently, the author believes that hsp 72/73 might be an important role of corneal haziness following PRK.