Changes of peripheral and bone marrow erythrocytesin plateau pikasand rats after hypoxia exposure / 中国组织工程研究

BACKGROUND: Changes of red blood cell in peripheral blood and bone marrow erythropoietic system in plateau pikas are of great significance for hypoxic adaptation. OBJECTIVE: To explore the hypoxic adaptation of erythropoietic system in plateau pikas by comparing the morphological changes of peripheral blood and bone marrow between plateau pikas and rats exposed to hypoxia. METHODS: There were 12 healthy wild plateau pikas and 12 clean Sprague-Dawley rats and were randomly divided into experimental and control groups, 6 in each group. The experimental group animals were fed in a simulated 5 000 m altitude hypobaric hypoxia chamber for 28 consecutive days, and the control group animals were fed in the laboratory at 2 260 m altitude. RESULTS AND CONCLUSION: (1) The diameter of the erythrocytes was smaller and red blood cell count was higher in plateau pikas than those in the rats of control group. After 28 days of hypoxia, red blood cell count, hemoglobin and hematocrit were increased in both experimental groups (P < 0.001), but the increased rate of plateau pikas were less than that of the rats. The mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration showed no significant changes in plateau pikas. (2) Results of bone marrow smear showed that the proportion of polychromatic and orthochromatic erythroblasts had no significant changes in plateau pikas after hypoxia, but increased significantly in the rats (P < 0.05). (3) Hematoxylin-eosin staining results of the sternum indicated that the immature erythroblasts islands did not change significantly in plateau pikas, but increased significantly in rats. (4) So the erythroid changes in peripheral blood and bone marrow of plateau pikas before and after hypoxia are significantly lower than those of the Sprague-Dawley rats, and they may be related to the hypoxia adaptation mechanism.

Material didáctico para la enseñanza aprendizaje del sistema hemolinfopoyético en la carrera Bioanálisis Clínico / Teaching aid for the teaching-learning of the hemolinfopoietic system in the Clinical Bioanalysis degree

RESUMEN Fundamento: la educación superior efectúa transformaciones profundas que se convierten en un factor clave para la puesta en marcha de procesos necesarios en el enfrentamiento a los desafíos del mundo existente. Objetivo: elaborar un material didáctico para la enseñanza aprendizaje del sistema hemolinfopoyético, con énfasis en el extendido de sangre periférica en la carrera de Bioanálisis Clínico. Métodos: se realizó un estudio descriptivo transversal en el período septiembre 2017-febrero 2018, en la Facultad Tecnológica "Octavio de la Concepción y de la Pedraja" de la Universidad de Ciencias Médicas "Carlos J. Finlay", de Camagüey. Se emplearon métodos teóricos: analítico-sintético e inductivo-deductivo; empíricos: observación a clases, la encuesta en forma de entrevista a docentes y la prueba exploratoria a estudiantes; y matemático-estadísticos para el cálculo de las frecuencias absolutas y el porcentaje. El producto fue valorado por criterios de especialistas. Resultados: se evidenciaron insuficiencias en el proceso enseñanza aprendizaje por los profesores para trabajar de manera integrada los procesos cognitivos sobre extendidos de sangre periférica como una vía para analizar las células hematopoyéticas; entre los estudiantes primaron el uso incorrecto de técnicas y procederes en los análisis y en la elaboración de los informes a partir de la observación e interpretación de extendidos de sangre periférica como componente esencial de la Hematología. Conclusiones: se elaboró un material didáctico que fue valorado por criterios de especialistas en las categorías de muy adecuado y adecuado en todos los indicadores propuestos, por lo que se consideró factible de ser aplicado.

ABSTRACT Background: Higher education carries out profound transformations that become a key factor for the implementation of necessary processes in the face of world challenges. Objective: to develop a teaching aid for the teaching - learning of the hemolinfopoietic system, with emphasis on the spread of peripheral blood in the Clinical Bioanalysis degree. Methods: a descriptive cross-sectional study was carried out from September 2017-to February 2018, at the "Octavio de la Concepción y de la Pedraja" Technological Faculty of the "Carlos J. Finlay" Camagüey University of Medical Sciences. Theoretical methods were used: analytical-synthetic and inductive-deductive; Empirical ones: observation to classes, the survey in the form of teacher interviews and the exploratory test to students; and mathematical-statistics for the calculation of the absolute frequencies and the percentage. The product was valued by criteria of specialists. Results: inadequacies in the teaching-learning process were evidenced by teachers to work in a comprehensive way on the cognitive processes on peripheral blood as a way to analyze hematopoietic cells; Among the students, the incorrect use of techniques and procedures prevailed in the analysis and in the preparation of the reports based on the observation and interpretation of peripheral blood spreads as an essential component of Hematology. Conclusions: a teaching aid was developed that was assessed by criteria of specialists in the categories of very adequate and adequate in all the proposed indicators, so it was considered feasible to be applied.

Analysis of hematopoietic system damage in 210 patients with brucellosis / 中华地方病学杂志

Objective The correlation between blood system damage and splenomegaly in brucellosis patients and their effects on liver function were analyzed.Methods Data were retrospectively collected in patients with blood system damage diagnosed of brucellosis from 2005 to 2016 at the Department of Infectious Disease of Harbin Medical University First Affiliated Hospital.At the same time,test results of the selected patients liver function,ferritin,D-dimer and abdominal ultrasonography were collected.According to splenomegaly or not,patients were divided into splenomegaly and no-splenomegaly,and any difference in hematopoietic damage caused by splenomegaly or not was analyzed.Results Of the 210 patients (101 cases of splenomegaly,109 cases without splenomegaly),170 were male (80.95％),40 were women (19.05％),and age was (39.65 ± 10.79) years.The patients with abnormal blood system were 103 cases (49.05％);in splenomegaly group there were 58 cases of blood system damage;in no-splenomegaly group there were 45 cases of blood system damage,and there was a statistically significant difference between the two groups (x2 =5.465,P ＜ 0.05);151 cases of brucellosis had elevated aminotransferase (71.90％),but transaminase elevated or not in different intervals of age groups was not statistically different between liver function and age (x2 =10.192,P ＞ 0.05).Ferritin increased in 26 cases (12.38％).D-dimer increased in 22 cases (10.48％).There were 93 patients with splenomegaly and transaminase elevation,and 58 patients with non-splenomegaly and transaminase elevation.There were significant differences between the two groups (x2 =39.204,P ＜ 0.05).Conclusion Brucellosis can cause blood system damage,probably caused by splenomegaly;and patients with brucellosis are often accompanied by elevated transaminases.

Retrospective analysis on outpatient visitors′parvovirus B19 infection in Fuzhou area from 2011 to 2016 / 中华检验医学杂志

Objective To investigate the outpatient visitor′s B19 infection in Fuzhou area, study the correlation between B19 virus infection and clinical diseases.Methods The infection status of B19V IgM and IgG in 22 089 outpatient visitors in Fuzhou area from 2011 to 2016 has been retrospectively analyzed.The patients were divided into different groups according to sex,age,different pregnant outcomes, healthy people and hematopoietic system diseases.Results The positive rate in 22 089 patients of B19V IgM was 4.5%(998/22 089)and the IgG positive rate was 36.9%(8 155/22 089); The positive rate of B19V IgM in female patients(4.8%,546/11 374)was higher than male patients(4.2%,452/10 715)(χ2=4.333,P<0.05); The middle-aged and elderly patients IgG positive rate(53.6%,3 629/6 772;54.3%,1 542/2 838)were significantly higher than infants,children and young people(36.0%,989/2 747;25.4%,237/934;20.0%,1 758/8 797); The positive rate of IgM in adverse pregnancy outcomes (8.2%,20/245)was higher than normal pregnant women(3.3%,23/688)(χ2=9.548,P<0.05).In pancytopenia,thrombocytopenia and anemia patients, the positive rates of B19V IgG were 39.8%(165/415),38.1%(297/780)and 35.4%(81/226)respectively, all of which were higher than that in the healthy people(14.4%,78/543)(χ2=80.127,88.626,43.461; P<0.05).Conclusions The outpatient visitor′s infection rate of B19V in Fuzhou is high.B19V is a common virus who can lead to adverse pregnancy outcomes.What′s more, it also can lead to pancytopenia, thrombocytopenia, anemia or other related hematopoietic diseases.

Protective effects of 3,3′-diindolylmethane on radiation damage of hematopoietic system in mice / 天津医药

Objective To investigate the protective effect of 3,3′-diindolylmethane (DIM) on radiation-induced injury in mouse hematopoietic system. Methods Thirty C57BL/6 mice were randomly divided into control group, 2 Gy irradiation group and 2 Gy irradiation+DIM group (n=10 for each group). Mice of control group received sham irradiation, and the other two groups accepted 2 Gy 137Cs γ-ray total body irradiation. Mice in 2 Gy irradiation +DIM group were intraperitoneally injected 75 mg/kg DIM 30 min before irradiation. Mice of other two groups were treated with reference solution. After 7 d and 15 d of 2 Gy irradiation, the peripheral blood samples were collected to count the number of bone marrow nuclear cells (BMNCs). The level of reactive oxygen species(ROS) was measured by DCFH-DA. The levels of colony forming units-granulocyte-macrophage (CFU-GM) were also detected. Results The numbers of white blood cell (WBC), platelet count (PLT), BMNCs and CFU-GM were significantly decreased and the ROS level of bone marrow cells increased significantly in the irradiated group than those of control group (P<0.05). Compared to 2 Gy irradiation group, the numbers of WBC, PLT, BMNCs and CFU-GM were significantly increased in 2 Gy irradiation +DIM group, and the level of ROS was decreased significantly (P<0.05). Conclusion DIM has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with the decreased ROS level.

Radioprotective effect of cimitidine on acutely irradiated mice survival and hematopoietic system / 解放军医学杂志

Objective To investigate the radioprotective effect of cimetidine on survival rate and hematopoietic system in acutely irradiated mice.Methods The total body irradiation doses were 6.0Gy and 8.0Gy respectively at 1.01Gy/min rate. Sixty healthy male C57BL/6 mice were randomly divided into control group, model group, positive-drug (523) group and cimetidine groups (33.3mg/kg, 100mg/kg and 300mg/kg). Each group had ten mice. The mice were given intragastric administration of cimetidine for 6d before the irradiation in cimetidine groups, and 523 was administered before irradiation once a day for one day in 523 group, and at 5h after irradiation, was given again. The 30d survival rate after 8.0Gy irradiation was recorded. The peripheral blood cells, bone marrow DNA content and frequency of micronucleated polychromatic erythrocytes (fMNPCE) were determined 30d after 6.0Gy irradiation.Results After 8.0Gy irradiation, all the mice died on 21th day in model control group. The survival rates in cimetidine groups were 50%, 20% and 30%, respectively. After 6.0Gy irradiation on 30th day, compared with control group, the peripheral white blood cells (WBC) and bone marrow DNA content were decreased significantly (P<0.01,P<0.05) in model group, and fMNPCE was increased significantly (P<0.05). Compared with model group, WBC was significantly increased in 300mg/kg cimetidine group (P<0.01). In cimetidine groups, the bone marrow DNA content was increased significantly after irradiation (P<0.01 orP<0.05), and the fMNPCE was decreased significantly (P<0.01 orP<0.05) and tended towards normal.Conclusion Cimetidine could improve 30d survival rate of acutely irradiated mice and has good protective effect on hematopoietic system.

Expression of retinoic acid receptor in the mouse embryonic hematopoiesis / 第二军医大学学报

Objective To investigate the expression of retinoic acid receptors (RARs) subtypes RAR-α, RAR-β, RAR-γ and retinal dehydrogenases 1(Ra/dh1), Raldh2 in the mouse embryonic hematopoiesis, so as to discuss the role of different RAR subtypes in embryonic hematopoiesis. Methods The yolk sac (YS) at embryonic day 9. 5 (E9. 5), aortagonad- mesonephros (AGM) region at E10. 5 and E11. 5, fetal liver (FL) at E13. 5, E14. 5 and E17. 5 were harvested from the pregnant mice. The bonemarrow (BM) was also harvested from adult mice. The mRNA levels of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 at the above four different hematopoietic sites were assessed by quantitative reverse transcription polymerase chain reaction (RT PCR). Results The mRNA levels of RAR-α, RAR-β, RAR-γ, RaldhX and Raldh2 at E9. 5 YS, E11. 5 AGM regions and BM were significantly higher than those in other relevant hematopoietic tissues (P<0. 01). Compared with the E10. 5 AGM region, E11. 5 AGM region had significantly increasedmRNA levels of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 (P<0. 01), but the mRNA levels of these genes in FL at E13. 5-E17. 5 were not significantly different. Conclusion The expressions of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 in YS, AGM regions and BM are consistent with the situation of colony forming unit erythroid (CFU-E), suggesting that retinoic acid is closely related to the formation of hematopoiesis in the YS, AGM region and BM in mouse, but has less correlation with the FL in mouse.

Protective effect of propofol on total body irradiation induced hematopoietic system injury in mice / 国际药学研究杂志

Objective To observe the protective effect of propofol on hematopoietic system injury in mice with total body irradiation (TBI). Method Three different radiation doses were used in the experiments: 7.5 Gy TBI in 30 day-survival experiment, 6Gy TBI in colony-forming unit spleen (CFU-S) experiment and 2Gy TBI in the other experiment; mice were divided into 4 groups in a 30 day-survival experiment, including 7.5Gy TBI group, 7.5 Gy TBI + 5 mg/kg propofol group, 7.5 Gy TBI + 10 mg/kg propofol group and 7.5 Gy TBI + 20 mg/kg propofol group. For the other experiments, mice were divided into 4 groups: control group, propofol group, TBI (2 or 6 Gy) group, and TBI + 20 mg/kg propofol group. Propofol of 20 mg/kg were administered to mice 1 d before TBI, 30 mins before TBI and once each day within the following 7 days after TBI. Mice were euthanized on the ninth day after TBI, the number of CFU-S, peripheral blood parameters and bone marrow cells per femur were measured in this experiment. Results Propofol improved the 30 day-survival of lethally irradiated mice. There were increases in number of CFU-S, white blood cells, red blood cells, hemoglobin in peripheral blood and bone marrow cells per femur in 2 Gy TBI + 20 mg/kg propofol group compared to 2 Gy TBI group (P<0.05). Conclusion Propofol exhibits a promising protective effect on TBI-induced hematopoietic system injury; further study should be focused on the related mechanisms.

Protective effect of propofol on total body irradiation induced hematopoietic system injury in mice / 国际药学研究杂志

Objective To observe the protective effect of propofol on hematopoietic system injury in mice with total body irra?diation(TBI). Method Three different radiation doses were used in the experiments:7.5 Gy TBI in 30 day-survival experiment,6Gy TBI in colony-forming unit spleen(CFU-S)experiment and 2Gy TBI in the other experiment;mice were divided into 4 groups in a 30 day-survival experiment,including 7.5Gy TBI group,7.5 Gy TBI+5 mg/kg propofol group,7.5 Gy TBI+10 mg/kg propofol group and 7.5 Gy TBI + 20 mg/kg propofol group. For the other experiments,mice were divided into 4 groups:control group,propofol group,TBI(2 or 6 Gy)group,and TBI+20 mg/kg propofol group. Propofol of 20 mg/kg were administered to mice 1 d before TBI,30 mins before TBI and once each day within the following 7 days after TBI. Mice were euthanized on the ninth day after TBI,the number of CFU-S,peripheral blood parameters and bone marrow cells per femur were measured in this experiment. Results Propofol im?proved the 30 day-survival of lethally irradiated mice. There were increases in number of CFU-S,white blood cells,red blood cells, hemoglobin in peripheral blood and bone marrow cells per femur in 2 Gy TBI+20 mg/kg propofol group compared to 2 Gy TBI group (P<0.05). Conclusion Propofol exhibits a promising protective effect on TBI-induced hematopoietic system injury;further study should be focused on the related mechanisms.

Effects of long-term consumption of Fallopia multiflora on mouse hematopoietic system / 中国比较医学杂志

Objective To investigate the effects of long-term consumption of Fallopia multiflora on mouse hematopoietic system.Methods Forty 10-month old female C57BL/6J mice were equally divided into two groups at random, the control group fed with normal food , and the experimental group , given food with added Fallopia multiflora. After 10 month, the mice were sacrificed, and the peripheral blood, spleen, thymus and bone marrow cells were examined by flow cytometry.Results In the mice fed with Fallopia multiflora, the percentage of B cells in the spleen and CD 4 +cells in the thymus were increased , and CD8 + cells in the thymus and bone marrow hematopoietic stem cells were decreased , among the bone marrow cells , G0 cells were increased , but G1 and G2/S/M cells decreased .Conclusions Long-term proper consumption of Fallopia multiflora can delay the ageing of the hematopoietic system , and sustain its stability.

Effect of the administration time of hs6101 on hematopoietic recovery in ICR mice injured by cyclophosphamide / 解放军医学杂志

Objective To explore the effect of the administration time of HS6101 on hematopoietic recovery in ICR mice injured by cyclophosphamide (CTX). Methods One hundred and three male ICR mice were divided into 4 groups: CTX control, HS6101 prevention, HS6101 treatment, and HS6101 prevention+treatment groups. CTX was intraperitoneally injected into the ICR mice at a dose of 100mg/(kg.d) for three consecutive days to establish a chemotherapeutics-injured model. HS6101 at a dose of 27μg/mouse in 0.2ml was subcutaneously injected into the mice 1h before the first administration of CTX in HS6101-preventiongroup, 1h after the last administration of CTX in HS6101 treatment group, and both at 1h before the first administration and 1h after the last administration of CTX in HS6101 prevention + treatment group. Physiological saline was subcutaneously injected into the mice in CTX control group (0.2ml/mouse). 10μl peripheral blood was collected from the caudal vein for WBC, neutrophil lymphocyte, RBC and platelet counts on day -1, 3, 5, 7, 9, 11, 13, 15, 17 with the MEK-7222K cell analyzer, and the cell count was compared between HS6101 treatment mice and CTX control mice. Another 30 male ICR mice were used for bone marrow colony forming unit (CFU) assay and bone marrow histopathological examination, and they were assigned into normal control, CTX control, HS6101 prevention, HS6101treatment and HS6101 prevention + treatment groups (each n=6). On the day 4 and day 9 after CTX injection, mice were sacrificed and bone marrow cells were collected from the left femur for mononuclear cell (MNC) isolation. 1×104 MNCs were planted in 1.0ml mouse CFU culture medium M3434 and cultured in incubator with the temperature of 37℃, and 5% CO2 for 7 days. After that, granulocyte macrophage-colony-forming unit (GM-CFU), megakaryocyte colony forming unit (MK-CFU), mixture-colony-forming unit (Mix-CFU), burst-forming unit-erythroid (BFU-E) and colony-forming unit-erythroid (CFU-E) were counted. Then the right femur was taken for histopathology examination. Results After CTX injection, counts of WBC, neutrophils, lymphocytes, RBC and platelets of all the mice decreased rapidly. However, the nadirs of WBC, neutrophils and lymphocytes counts in HS6101 prevention group were higher than those in CTX control group, and the counts on day 3 were higher than those in HS6101 treatment group and HS6101 prevention+ treatment group. On day 3, RBC count in HS6101 prevention group was the highest. It was higher on day 5 and day 7 than that of mice in CTX group. In addition, the platelet count in HS6101 prevention group was also the highest on day 3, although that in HS6101 treatment group and 6101 prevention + treatment group was lower than CTX control group. Bone marrow colony forming unit assay showed that the counts of GM-CFU, MK-CFU, BFU-E and CFU-E in all the HS6101 treatment mice were significantly higher than those in CTX control mice. On day 4, histopathological examination of bone marrow from HS6101-treated mice displayed more intact architecture compared with CTX control mice. Three of eighteen (3/18) mice died in HS6101 treatment group, and nine of eighteen (9/18) died in HS6101 prevention + treatment group, suggesting that HS6101 should not be administered after CTX injection. Conclusion Administration of HS6101 at 1h before giving CTX could significantly promote hematopoietic recovery in ICR mice injured by CTX.

Obesity and inflammation and the effect on the hematopoietic system

Bone marrow is organized in specialized microenvironments known as 'marrow niches'. These are important for the maintenance of stem cells and their hematopoietic progenitors whose homeostasis also depends on other cell types present in the tissue. Extrinsic factors, such as infection and inflammatory states, may affect this system by causing cytokine dysregulation (imbalance in cytokine production) and changes in cell proliferation and self-renewal rates, and may also induce changes in the metabolism and cell cycle. Known to relate to chronic inflammation, obesity is responsible for systemic changes that are best studied in the cardiovascular system. Little is known regarding the changes in the hematopoietic system induced by the inflammatory state carried by obesity or the cell and molecular mechanisms involved. The understanding of the biological behavior of hematopoietic stem cells under obesity-induced chronic inflammation could help elucidate the pathophysiological mechanisms involved in other inflammatory processes, such as neoplastic diseases and bone marrow failure syndromes.

The detection of peripheral blood smear and bone marrow examination in patients with panhematopenia and its clinical significance / 中国基层医药

Objective To explore the clinical significance of peripheral blood smear and bone marrow examination in the etiological diagnosis of panhematopenia.Methods 180 cases of panhematopenia were selected and were given peripheral blood smear and bone marrow examination,and the results were analyzed.Results There were 50 cases of panhematopenia because of non hematopoietic system diseases.Among 50 cases of non-hematopoietic system disease,there were 20 cases of chronic hepatitis,3 cases of cirrhosis,4 cases of lung cancer,10 cases of malignant tumors,3 cases of endocarditis,7 cases of connective tissue-like illness,1 case of gastric cancer,1 case of systemic lupus erythematosus and 1 case of hypersplenism.Due to diseases of the hematopoietic system,there were 130 cases of panbematopenia.Among 130 cases of panhematopenia due to diseases of the hematopoietic system,there were 74 cases of acute leukemia(AL),13 cases of myelodysplastic syndrome(MDS),13 cases of aplastic anemia(AA) and 30 cases of megaloblastic anemia(MA).Conclusion Peripheral blood smear and bone marrow examination is conducive to diagnose the cause of the panhematopenia.Bone marrow examination could improve the accuracy rate of panhematopenia.

La Biometría hemática desde la optica de la homeopatía: hemograma, citometríahemática, citología hemática / The biometrics from the viewpoint of homeopathy: CBC, citometría hemática, hematic cytology

Las pruebas de laboratorio son un complemento importante para que el médico homeópata recopile la totalidad de los datos con que se manifiesta la enfermedad de sus pacientes. Uno de los exámenes más habituales a los que se puede recurrir es la biometría hemática, el cual ofrece una orientación conveniente sobre los mecanismos que se activan en el organismo ante un estímulo nocivo determinado,mediante información detallada sobre los elementos formes de la sangre: serie blanca, serie roja y plaquetas. Se presentan a continuación las consideraciones más importantes sobre las variables que se estudian mediante un hemograma, pues aunque ninguno delos valores que nos puede proporcionar se encuentra en los repertorios clásicos,es evidente que nos ofrece información sobre signos tan importantes o comunescomo anemia, hemorragias, trombosis, tromboflebitis o abscesos, todos ellos ensus diferentes modalidades.

Laboratory tests are an important complement to the homeopath. They help to collect all the data manifested by the patient´s disease. One of the most common tests to which the homeopath can turn to is the Cell Blood Count, which offers a convenient guidance of the mechanisms that are activated in the body to a determined noxious stimulus, through detailed information about the blood cells: white,red and platelets count.The following are the most important considerations of the variables that are studied by a CBC, as though none of the values that we can find is in the classical repertoire, it is evident that offers very important information on common signs such as anemia, bleeding, thrombosis, thrombophlebitis or abscess, all in their different ways.

Anemias sideroblásticas y puenteado basófilo: Indicador biológico por exposición ocupacional al plomo y sus derivados / Sideroblastic anemia and basophilic bridged: Biological indicator of occupational exposure to lead and its derivatives

En el presente estudio se evaluaron 34 individuos de ambos géneros (n = 15 mujeres y n = 19 hombres) con edades promedios de 37,35 ± 10,37. De los cuales 09 trabajadores de talleres mecánicos y 25 de imprentas gráficas con exposición laboral entre 8 a 10 horas/día. Bajo su consentimiento se les tomo muestra de sangre del antebrazo derecho para evaluar hematológica y morfológicamente glóbulos rojos (punteado basófilo), glóbulos blancos (segmentados neutrofilos), plaquetas y determinar por espectroscopia de absorción atómica con atomización elec trotérmica niveles séricos de plomo. Los resultados analíticos obtenidos para niveles de plomo globales expresados en μgL-1 de 36,03 ± 23,02 evidenciaron correlación directa y positiva con los parámetros bioquímicos evaluados. Ma yo res concentraciones de plomo en sangre coincidieron con pun teados basófilos toscos y alteraciones cualitativas morfológicas tales como hipocromía moderada en glóbulos rojos, granulaciones tóxicas e hipersegmentación en segmentados neutrofilos. No se observaron diferencias estadísticamente significativas con un p = 0,002 entre los grupos expuestos y tiempo de exposición por jornada laboral, más si entre los géneros con un p = 0,087, siendo más evidente el impacto de la exposición ocupacional en hombres, asumiendo mayor masa corporal y por ende mayor densidad ósea por donde este metal tóxico tiene un 95% de afinidad, además de contar el género masculino con mayor producción hematopoyética (La cantidad considerada normal fluctúa entre 4.500.000 (en la mujer) y 5.000.000 (en el hombre) por milímetro cúbico (o microlitro) de sangre). Los resultados obtenidos constituyen una herramienta útil para un pre-diagnóstico a exposición o intoxicación por plomo cuando por infraestructura no se cuente en laboratorios bioanalíticos con un equipo de espectroscopia de ab sorción atómica con atomización electrotérmica.

This study assessed 34 individuals of both genre (n = 15 women and n = 19 men) aged averages of 37.35 ± 10.37. Of whom 09 workers of garages and 25 printing graphs with occupational exposure between 8 to 10 hours per day. Under their consent took them right forearm blood sample to evaluate haematological and morphologically (stippling Basophilic) red blood cells, white blood cells (segmented neutrophils), pla te lets and by Atomic Spectrometry atomization absorption spectroscopy to determine serum levels of lead. The analytical re sults for overall lead levels ex pressed in μgL-1 36.03 ± 23.02 demonstrate positive and direct correlation with the biochemical parameters evaluated. High concentrations of lead in blood coincided with various crude basophiles and qualitative morphological alterations such as hypochromia moderate red globules, toxic granulation’s and hypersegmentation in segmented neutrophils. There were no statistically significant differences with p = 0.002 among exposed groups and exposure time by working day, more if genre with a p = 0.087, being most evi dent impact of occupational exposure in men, assuming greater mass body and therefore greater bone density where this toxic metal has a 95% of affinity’s well as the masculine gender with greater production hematopoietic (considered normal amount fluctuates between 4.500.000 (in women) and 5.000.000 (in humans) per cubic millimeter (microliter) of blood). The results constitute a useful tool for an prediag nostic to exposure or poisoning by lead when infrastructure don’t count in laboratories bioanalytic’s with a team of atomic absorption spectroscopy with spectrometry atomization.

Negative effects of protein tyrosine phosphatase SHP-1 in cell signaling / 国际肿瘤学杂志

SHP-1 is the protein tyrosine phosphatase which is mainly expressed in the cytoplasm of hematopoietic-derived cells,and it is the key factor to change the levels of intracellular phosphorylation.SHP-1 can generate different intracellular signals in different cells,and it can play various functions.

Protective effect of CpG-ODN against irradiation injury of bonemarrowhematopoietic system in mice / 第二军医大学学报

Objective To study the therapeutic effect of CpG-ODN, an agonist of Toll-like receptor 9 (TLR9), on irradiation injury of bone marrow hematopoietic system in mice. Methods Mice were treated with intraperitoneal injection of CpG-ODN (50 μg each) at 30 min, 24 h and 48 h after irradiation. The survival rates of animals were observed after irradiation with different doses, the numbers of white blood cell (WBC) and bone marrow nucleated cells within a certain period of time were observed, the bone marrow was pathologically studied, and the number of endogenous colony forming unit-spleen (endoCFU-S) was counted. Results Our results showed that intraperitoneal injeciion of CpG-ODN significantly improved the survival rate of mice and increased the numbers of peripheral WBC and bone marrow nucleated cells (P<0.01); moreover, it also ameliorated the pathological injury of the bone marrow and reduced the death of bone marrow stem cells. Conclusion Intraperitoneal CpG-ODN injection can ameliorate the irradiation injury of bone marrow hematopoietic system in mice.

A telomerase em células-tronco hematopoéticas: [revisão] / Telomerase in hematopoietic stem cells: [review]

A proliferação das células-tronco hematopoéticas sofre a perda dos telômeros a cada divisão celular. Alguns autores discordam quanto à perda ou não do potencial proliferativo e capacidade de auto-renovação das células mais diferenciadas. Revisaremos aqui o papel da telomerase na biologia do sistema hematopoético, na diferenciação normal ou maligna, assim como no envelhecimento das células-tronco hematopoéticas. A constante renovação celular requerida pela hematopoese confere às células-tronco embrionárias, assim como à maioria das células tumorais, um aumento da capacidade proliferativa marcada pela detecção da enzima telomerase e possível manutenção dos telômeros. Estudos clínicos se farão necessários para esclarecer melhor a atividade da telomerase em células-tronco hematopoéticas, seu possível uso como marcador de diagnóstico e seu uso a fim de propósitos prognósticos.

Hematopoietic stem cell proliferation leads to telomere length decreases at each cellular division. Some authors disagree about the telomere influence on the reduction of the proliferative potential and capacity of self renewal. Here we review telomerase function in the biology of the hematopoietic system, in normal or differentiation and its influence on the ageing of hematopoietic stem cells. The constant cellular renewal required to maintain the hematopoietic system, provides embryonic stem cells, as well as malignant cells, an increased proliferative capacity. This is marked by the detection of telomerase enzyme activity and possible telomere maintenance. Clinical trials will be required to clarify telomerase activity in hematopoietic stem cells, its possible use as a diagnostic marker and its use for prognostic purposes.

Diagnostic and Therapeutic Management of Acute Radiation Syndrome and Internal Contamination

The ARS(Acute Radiation Syndrome) develops, within 60 days after exposure to ionising radiation with typical clinical signs and symptoms as a function of time. The interactions and combined effects of radiation-induced damage to different organ systems are diverse and not yet fully understood. Therefore, when accidental exposure to ionising radiation is documented or suspected, guidance for immediate diagnostic procedures and specialised care are required to handle the complexity of the ARS. The following four organ systems, Neurovascular system (N), Hematopoietic system (H), Cutaneous system (C) and Gastrointestinal system (G) are considered to be of critical significance for the development of ARS and should therefore receive special attention in the medical management of radiation accident cases. The Assessment of the severity of damage, Decision on the kind of hospitalisation, Provision of appropriate therapeutic interventions and Evaluation of the patient's prognosis must be considered in the management of a patient after a radiation accident. When significant levels of radioactive materials are incorporated, pathological consequences may ensue, making emergent treatment particularly important. However, this should not take priority over treatment of life threatening conditions and of acute injuries. Following medical stabilization, careful radiological assessment can be performed to determine the presence of both external and internal contamination. It is important to note that 1) contaminated patients do not represent a direct hazard to health care providers and 2) lifesaving procedures should not be delayed regardless of the level of contamination.

Accelerating hematopoietic recovery in mice after allogeneic bone marrow transplantation by co-infusion of bone marrow mesenchymal stem cells / 中国医师杂志

Objective To investigate the effect of co transfusion of bone marrow mesenchymal stem cells(BMMSC) on hematopoietic recovery in mice after allogeneic bone marrow transplantation(allo-BMT).Methods Both BMMSC obtained after three to four weeks of culture and bone marrow cells of donor mice C57BL/6(H-2~b) were transplanted into the recipient mice BalB/c(H-2~d) that were lethally irradiated.Peripheral blood cells were counted on day 1,7,and 14 post-transplantation.CFU-S in recipient bone marrows were measured on day 7 and 14.Results The numbers of peripheral WBC,RBC,platelets and the number of CFU-S in recipient bone marrows on day 7 and 14 were all significantly higher in the co-infusion group than those in control group(P