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Objective To explore the protective effect of Qihuo decoction (QHD) on the livers of sleep-deprived rats housed in the humid and hot environment, and to characterize ultrastructural changes of livers in these rats. Methods Forty male SD rats were randomized into 4 groups (10 each group): blank control group (BC group), humid and hot control group (HC group), humid and hot with sleep deprivation group (HS group), and humid and hot with sleep-deprived treated with QHD group (HSQ group). Rats in BC group were housed in the normal temperature and humidity chamber; while rats in HC group, HS group, and HSQ group were housed in the simulated hot and humid chamber. Rats in BC group, HC group, and HS group were given distilled water to gavage for 15 days, and rats in HSQ group were given QHD to gavage for 15 days. Then rats in HS group and HSQ group were sleep deprived for 72 hours. The serum concentrations of alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) were detected. The liver tissues contents of hepatic glycogen, malondialdehyde (MDA) and reduced glutathione (GSH) were detected. The expression of β-galactosidase (β-gal) in each group was also detected. Lastly, the rat hepatocytes ultrastructure was observed by a transmission electron microscope. Results In BC group, HC group, HS group, and HSQ group, the serum AST levels were (107.7±8.4)U/L, (126.9±10.4)U/L, (176.9±13.1)U/L, and (140.6±9.6)U/L; the ALT levels were (37.9±3.1)U/L, (45.5±6.2)U/L, (86.5±10.4)U/L, and (64.9±8.7)U/L. Statistical analysis among groups were significantly different (P<0.01). Compared with BC group, the levels of AST and ALT increased significantly in the rest 3 groups (P<0.01). The serum concentrations of AST and ALT in HSQ group were significantly lower than those in HS group (P<0.01). The hepatic glycogen levels were (19.7±1.5)nmol/mg prot, (17.9±0.8) nmol/mg prot, (11.3±0.5)nmol/mg prot, (14.6±0.7)nmol/ mg prot successively in the four groups. The MDA levels were (10.3±1.1)nmol/mg prot, (26.6±1.3)nmol/mg prot, (41.4±2.4) nmol/mg prot, (33.7±1.7)nmol/mg prot successively in the four groups. The GSH levels were (77.6±5.2)U/mg prot, (70.6±5.1) U/mg prot, (45.8±3.8)U/mg prot, (60.7±3.3)U/mg prot successively in the four groups. Comparisons among groups were significantly different (P<0.01). Compared with BC group, the data of HC group, HS group and HSQ group about the contents of hepatic glycogen and GSH decreased significantly (P<0.05), the contents of MDA increased significantly (P<0.01). Compared with HS group, the data of HSQ group about the contents of hepatic glycogen and GSH increased significantly (P<0.01), the contents of MDA decreased significantly (P<0.01). The counts of β-gal positive cells were (0.67±0.52), (6.23±0.42), (13.5±0.48) and (10.16±0.36) successively in rats liver tissues of the four groups. Comparison among groups was significantly different (P<0.01). Compared with the BC group, the data of the HC group, HS group, and HSQ group about the number of β-gal positive cells increased significantly (P<0.01). The number of β-gal positive cells in HSQ group was lower than that in HS group (P<0.01). Light microscopy and transmission electron microscopy showed that the hepatocytes of HC group, HS group and HSQ group were damaged to varying degrees, and the injury of HS group was the most obvious, and the injury of HSQ group was reduced. Conclusion QHD Can obviously alleviate the degree of liver injury that was induced by high temperature and high humidity and sleep deprivation, and the mechanism of which might be related to increasing hepatic glycogen reserve, balancing oxidative stress and slowing process of hepatic cell aging.
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Objective To study the relevance of 1iver fibrosis at Kasai procedure with age, degree of pathological changes of liver tissue, header bile ductuler proliferation and pathological feature of portal fibrous tissue in biliary atresia patients. Methods From January 2013 to June 2016, 48 biliary atresia patients accepted Kasai procedure, and liver tissue biopsy and portal fibrous tissue excision was done. Level of liver fibrosis was evaluated under microscope using Masson trichrome staining and HE staining, and extent of ductular reaction was evaluated using CKl9 immunohistochemistry staining with image analysis technology. Based on classification of Okamoto severity degree of liver fibrosis, forty eight patients were divided into two groups:low-grade liver fibrosis group (23 patients)and severe liver fibrosis group (25 patients). The relevance of age at Kasai procedure, liver pathological changes, header bile ductuler proliferation, portal fibrous tissue in volume and bile ductuler proliferation, inflammation reaction with severity degree of liver fibrosis was analyzed with SPSSl6.0 software statistically. Results Age of severe 1iver fibrosis group at Kasai procedure were 38- 89 d, and the mean age was (62.64 ± 14.92) d. Age of low-grade 1iver fibrosis group were 18- 92 d, and the mean age was (53.13 ± 16.89) d. There was significant difference (t=2.071, P=0.044). Mean number of patients with header bile ductuler proliferation at Kasai procedure in severe 1iver fibrosis group and low-grade 1iver fibrosis group were (17.28 ± 4.31) cases and (11.39 ± 4.00) cases, and there was significant difference (t=4.900, P=0.000). There was significant difference in degree of hepatocellular pathological changes at Kasai procedure between groups (χ2 =4.691, P=0.030). There were no significant differences in the volume of portal fibrous tissue in volume (t=0.276, P=0.784), portal bile ducts proliferation (t=0.53, P=0.60) and inflammation reaction (χ2=3.074, P=0.080) between groups. Conclusions There is significant relevance between the degree of 1iver fibrosis in biliary atresia patients at Kasai procedure and age, level of hepatocellular pathological change and degree of header bile ductuler proliferation , The more older the age, the more sever the hepatocellular pathological change and the bile ductuler proliferation, the more sever the liver fibrous.
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Objective To investigate the protective effects of Apigenin (APG) on hepatic in diabetic rats.Methods The experimental diabetes model were made by intraperitoneal injecting STZ. The rats were randomly devided into six groups: a normal control group, a diabetes model control group, APG 5, 10, 20 and 40 mg/kg treated groups, and the drug was given by intraperitoneal injection. 4 weeks later, the body weight, ratio of hepatic weight and body weight were detected; the histopathological changes of hepatic tissue were observed by HE staining; the content of ALT/AST/LDH in serum were determined; the activity of SOD/GSH-Px/CAT and the content of MDA in hepatic tissue were determined.Results Compared with the diabetes model control group, the body weight of APG(10, 20, 40) mg/kg treated groups were significantly increased (261.3 ± 15.8 g, 274.2 ± 18.4 g, 265.9 ± 19.0 gvs. 250.8 ± 21.4 g,P<0.05 orP<0.01), and the ratio of hepatic weight and body weight were significantly decreased (27.7 ± 5.69, 26.2 ± 4.91, 27.3 ± 4.58vs. 32.9 ± 5.85,P<0.05 orP<0.01); the hepatic tissue histopathological changes of APG-treated groups were significantly improved; the level of ALT in serum of APG (5, 10, 20 and 40) mg/kg treated groups were significantly decreased (1 039.3 ± 453.9 U/L, 917.6 ± 445.2 U/L, 828.4 ± 309.5 U/L, 721.7 ± 318.3 U/Lvs. 1 205.2 ± 484.1 U/L;P<0.05 orP<0.01), AST were significantly decreased (97.8 ± 23.8 U/L, 90.1 ± 19.6 U/L, 81.7 ± 15.7 U/L, 86.4 ± 19.2 U/Lvs. 105.3 ± 25.7 U/L;P<0.05,P<0.01), LDH were also significantly decreased (983.7 ± 192.6 U/L, 918.3 ± 212.9 U/L, 830.4 ± 174.2 U/L, 871.8 ± 183.1 U/Lvs. 1 102.8 ± 211.6 U/L;P<0.05 orP<0.01); the activity of SOD in hepatic tissue of APG (10, 20, 40) mg/kg treated groups were significantly increased (10.5 ± 1.9 U/mg, 11.6 ± 2.1 U/mg, 10.5 ± 2.0 U/mgvs. 9.1 ± 1.8 U/mg,P<0.05,P<0.01), GSH-Px were significantly increased (14.2 ± 2.7 U/mg, 15.3 ± 2.9 U/mg, 14.6 ± 2.6 U/mgvs. 12.9 ± 2.3 U/mg;P<0.05, P<0.01), CAT were significantly increased (3.15 ± 0.90 U/mg, 3.58 ± 0.88 U/mg, 3.31 ± 1.09 U/mgvs. 2.58 ± 0.79 U/mg,P<0.05 orP<0.01), and the content of MDA was significantly decreased (5.03 ± 1.70 nmol/mg, 4.66 ± 1.51 nmol/mg, 4.73 ± 1.65 nmol/mg vs. 5.98 ± 1.62 nmol/mg;P<0.05 orP<0.01). And the treatment effect of APG 20 mg/kg groups was the most significant.Conclusion APG had antioxidant enzyme activity, besides its reducing the damage of free radical, and protecting the hepatic tissue of diabetic rats effects.
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The aim of this research was to investigate the effect of testosterone propionate administration in the liver of rats. The rats were divided in the following groups: Initial control (SC), Aged control (SE) and Anabolic group (SA). Testosterone propionate was administered three times per week during 16 weeks. Using morphoquantitative techniques, we quantified the volume densities of lobular and non-lobular parenchyma, area and number of nuclei of hepatocytes. The data were analyzed statistically using mean and standard deviation, ANOVA one-way and level of significance about p0.05. Our results showed an increase in capillaries, perisinusoidal spaces and biliary ducts in SE group compared to SC. SA group showed a decrease in hepatic cells, non-lobular volume density and hepatocytes nuclei area, but also an increase in capillaries, perisinusoidal spaces, biliary ducts, number of hepatocytes and non-hepatocyte nuclei compared to SC. We conclude that a direct toxicity may have occurred, with consequent loss of the cells.
El objetivo fue investigar el efecto de la administración de propionato de testosterona en el hígado de ratas. Las ratas se dividieron en los siguientes grupos: control inicial (CI), control de Edad (CE) y grupo anabólico (GA). El propionato de testosterona se administró tres veces por semana durante 16 semanas. Utilizando técnicas morfocuantitativas, determinamos las densidades de volumen del parénquima lobular y no lobular, área y número de núcleos de los hepatocitos. Los datos fueron analizados estadísticamente con la media y desviación estándar, la prueba de ANOVA de una vía y un nivel de significación p0,05. Nuestros resultados mostraron un aumento en los capilares, espacios perisinusoidales y conductos biliares en el grupo CE en comparación con CI. El GA mostró una disminución en las células hepáticas, la densidad de volumen no lobular y el área de los núcleos de hepatocitos, como también un aumento en los capilares, espacios perisinusoidales, conductos biliares, número de hepatocitos y núcleos no hepatocíticos en comparación AL CI. Concluimos que una toxicidad directa puede haber ocurrido, con la consiguiente pérdida de las células.
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Animals , Male , Rats , Testosterone Propionate/pharmacology , Liver/drug effects , Aging , Analysis of Variance , Rats, Wistar , Hepatocytes/drug effects , Testosterone Propionate/administration & dosageABSTRACT
Liver transplantation is the only known treatment for patients with end-stage liver failure, but this therapy is limited by the shortage of donor organs. Hepatic tissue engineering combining biomaterial scaffolds and cells have been used as a promising strategy to create engineered liver graft for liver regeneration. Despite significant progress in this field, attempts to create clinically transplantable whole organs have not been as nearly successful. Recently, whole organ decellularization techniques have emerged as a new therapeutic strategy for organ replacement and provided feasibility for clinical translation. The perfusion decellularization method was applied to the whole organ for efficient removal of cellular components and generated organ scaffolds that can maintain the extracellular matrix (ECM) and vascular structure of the native organ. This review paper describes current progress in organ bioengineering for the development of transplantable liver grafts.
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Humans , Bioengineering , Extracellular Matrix , Liver , Liver Failure , Liver Regeneration , Liver Transplantation , Organ Transplantation , Perfusion , Tissue Donors , Tissue Engineering , TransplantsABSTRACT
HPLC method is quick,accurate,and sensitive,and can be applied to the determination of riboflavin in hepatic tissue.
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Objective:To detect the relation of peroxisome proliferators actived receptor ?(PPAR?)in the hepatic tissue of chronic hepatitis B and explore its significance.Methods:The protein and mRNA expression of PPAR? was detected by means of immunohistochemistry and semi-quantitative RT-PCR in cases of hepatic fibrosis staging S0-S4 of chronic hepatitis B and cases of hepatic hemangioma.The average of A values was obtained by image analysis.Results:There was not obvious correlation between PPAR? level and the hepatic activity index.Positive staining of PPAR? was observed in all of the human liver tissues.The expression of PPAR?in tissues of staging S0 and normal liver was stronger than that in tissues of staging S3 and staging S4(P
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Objective This study was designed to analyze the proteome differences between cancer tissue and surrounding-cancer tissue using Two-Dimensional Gel Electrophoresis (2-DE) in patients with HBV relative HCC.Methods Immobile phase pH gradients (IPGs) for isoelectric focusing of proteins were used as the first dimension,and SDS-polyacrglamide gel electrophoresis(PAGE) as the second dimension. The gels were stained by silver, scanned by ImageScanner, analyzed with ImageMast software.Results Average spots expressed in cancer tissue,cirrhosis tissue and chronic hepatitis tissue were significantly different(P