ABSTRACT
】 Objective To observe the effects and explore the mechanism of Huaxian Capsule (HXC) on pulmonary fibrosis. Methods 60 rats were divided into the normal group, the HXC group, the prednisone group, and the model group, 15 rats in each group. The pulmonary fibrosis model of rats was established with bleomycin. The experimental rats were sacrificed in batch on the 7th, 14th and 28th day for determining TIMP-1 protein and MMP-2 mRNA expression by immunohistochemistry and in situ hybridization respectively. The outcomes were compared between groups. Results It was showed that TIMP-1 protein and MMP-2 mRNA expression in different stage of pulmonary fibrosis increased significantly and continuously after modeling, showing significant difference in comparison between the model group and the normal group. After HXC treatment, the two indexes expressed weakly as compared with those in the model group, and showed significant difference to those in the model group on 14th and 28th. Conclusion HXC influence on the expression of TIMP-1 and MMP-2 mRNA in rats with pulmonary fibrosis. 【
ABSTRACT
Objective:To study on effect of Danshao Huaxian Capsule on proliferation of activated hepatic stellate cells(HSCs)in vivo and in vitro.Methods:(1)In vitro experiment:57 male Wistar rats were divided into a normal group,a model group and a treatment group.The rats of model group and treatment group were made hepatic fibrosis model with complex stimulation of CCl_4,drinking alcohol,high lipids and low protein diet for 8 weeks.The treatment group were treated for 8 weeks with intragastric perfusion of Danshao Huaxian Capsule,1g/kg.At the end of experiment,a part of the rats were used for detection of hepatic functions and hepatic fibrosis degrees,and another part of the rats were used to separate HSCs.Cell cycle percentage was detected with flow cytometry.(2)In vivo experiment:prepare Danshao Huaxian Capsule serum of the rat;separate cultured HSCs of the rat and divide into calf serum group,normal rat serum group and Danshao Huaxian Capsule serum group.5%,10% and 20% of the above serum was respectively added into the cultured HSCs.Primary HSCs proliferation was detected with MTT method.Results:(1)In vitro experiment:The hepatic function,hepatic fibrosis level and HSCs proliferation reduced significantly.(2)In vivo experiment: Danshao Huaxian Capstde serum group could significantly inhibit proliferation of HSCs as compared with calf serum group and normal rat serum group at the same content.The action showed dose-dependence.Conclusion:Danshao Huaxian Capsule can obviously inhibit proliferation of HSCs in vivo and in vitro.