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@#Objective To analyze the related knowledge-attitude-practice(KAP)and the influencing factors of human papilloma virus(HPV)vaccine among 16 — 45 year old resident women in Pudong New Area.Methods Six of the 36streets(towns)in Pudong New Area were randomly selected as the survey site,the resident women aged 16 — 45 years were randomly selected excluding those with reading or comprehension disabilities and those with mental disorders,and a total of 1 022 valid questionnaires were collected through self-filling questionnaires to understand the KAP status of the HPV vaccine among the resident women in Pudong New Area. The relationship among KAP of HPV vaccine and its influencing factors were analyzed by univariate analysis and structural equation model.Results The overall awareness rate of HPV vaccine among 16-45 year old resident women in Pudong area was higher. Univariate analysis showed that marital status,educational level,employment status and household annual income were related to the awareness level of HPV vaccine（χ~2=12. 832,17. 636,16. 770 and 20. 030,respectively,each P < 0. 05）;Age,marital status,employment status and children′s status were correlated with HPV vaccination level（χ~2= 12. 382,25. 777,8. 830 and 20. 138,respectively,each P <0. 05）;HPV vaccine health education,HPV and HPV vaccine knowledge scores affected HPV vaccination status（χ~2=97. 561 and 68. 969,respectively,P < 0. 001）;Subjects' knowledge of cervical cancer was positively affected by knowledge of HPV infection(γ_(11)= 0. 756,P < 0. 001). Knowledge of cervical cancer not only positively affected subjects′ attitudes towards the efficacy of HPV vaccine(β_(21)= 0. 557,P < 0. 001),also had a direct effect on the HPV vaccination behavior of the subjects,showing a promoting effect(β_(31)= 0. 274,P = 0. 004). Subjects′ approval of the efficacy of HPV vaccine had a positive effect on their actual vaccination behavior(β_(32)= 0. 175,P = 0. 016).Conclusion The willingness of the 16 — 45 year old resident women in Pudong New Area to inoculate HPV vaccine was positive,but the actual vaccination rate was low. It is suggested to strengthen the HPV vaccine publicity while strengthening the education of cervical cancer and HPV infection,and consider the necessity of including the suitable age males in the scope of human vaccination.
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@#Objective To investigate the effect of silencing E6-associated protein(E6AP)on the level of p53 protein in human papilloma virus(HPV)negative cervical cancer cells(C33A cells).Methods The siRNA sequence silencing E6AP(siE6AP)and silencing control disordered siRNA sequence(siControl)were transfected into C33A cells with the mediation of LipofectamineTM2000 transfection reagent respectively.The silencing effect of siRNA on E6AP and the expression of p53and cleaved-caspase-3 proteins were detected by Western blot.Results The levels of E6AP protein in C33A cells of siE6AP group were significantly lower(t =-4.597,P<0.05),while the levels of p53 and cleaved-caspase-3 proteins were significantly higher than those of siControl group(t = 4.533 and 7.099 respectively,each P<0.05).Conclusion Silencing of E6AP significantly increased the expression of p53 protein in C33A cells,indicating that silencing of E6AP may restore the activity and function of p53 protein in C33A cells.
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In May 2018， the World Health Organization （WHO） called for the "global elimination of cervical cancer" and China committed to join 194 countries worldwide to "eliminate cervical cancer". In November 2020， WHO released the Global Strategy for Accelerating the Elimination of Cervical Cancer， specifying the 2030 strategic goal. China has a large population， which makes the task of eliminating cervical cancer difficult. Based on the global and local burden of cervical cancer，we discussed the current situation of cervical cancer in China in the achievement of the elimination. We showed that in order to achieve the phased elimination goal of cervical cancer by 2030， the supply of HPV vaccine needs to increase by 38% every year， the HPV vaccination coverage needs to increase by 45% every year， and the screening coverage of cervical cancer in appropriate-aged women needs to increase by 10% every year. In conclusion， efforts should be made to expand cervical cancer screening， and to incorporate HPV vaccination into the national immunization program.
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In May 2018， the World Health Organization （WHO） called for the "global elimination of cervical cancer" and China committed to join 194 countries worldwide to "eliminate cervical cancer". In November 2020， WHO released the Global Strategy for Accelerating the Elimination of Cervical Cancer， specifying the 2030 strategic goal. China has a large population， which makes the task of eliminating cervical cancer difficult. Based on the global and local burden of cervical cancer，we discussed the current situation of cervical cancer in China in the achievement of the elimination. We showed that in order to achieve the phased elimination goal of cervical cancer by 2030， the supply of HPV vaccine needs to increase by 38% every year， the HPV vaccination coverage needs to increase by 45% every year， and the screening coverage of cervical cancer in appropriate-aged women needs to increase by 10% every year. In conclusion， efforts should be made to expand cervical cancer screening， and to incorporate HPV vaccination into the national immunization program.
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PURPOSE: This study aimed to evaluate the performance of the PANArray human papilloma virus (HPV) test, a PCR-based DNA microarray assay, in detecting HPV from patient samples and its concordance with the cobas 4800 HPV and Hybrid Capture 2 (HC2) tests. MATERIALS AND METHODS: The PANArray HPV, cobas 4800 HPV, and HC2 tests were performed on 504 cervical swab samples from patients with atypical cells of undetermined significance at five hospitals. The samples that were interpreted as ‘HPV-other’ type positive in the PANArray HPV test were confirmed by direct sequencing. RESULTS: The concordance rates were 80.8% between the cobas 4800 HPV and PANArray HPV tests [κ=0.59, 95% confidence interval (CI) 0.52–0.66] and 80.2% (κ=0.6, 95% CI 0.55–0.68) between the HC2 and PANArray HPV tests. Among the 62 patients negative on PANArray HPV (defined as the absence of high risk HPV), but positive on both cobas 4800 HPV and HC2 tests, 42 (67.7%) tested positive for ‘HPV-other’ types on the PANArray HPV test, and 31 (50.0%) had gray zone results [relative light unit/control (RLU/CO), 1.4–9.25] in the HC2 test. Of the patients deemed positive by the PANArray HPV test, 43 tested positive for high-risk (HR) HPV in cobas 4800 HPV and HC 2 tests. Among them, 58.2% showed HR HPV, including HPV 16, by direct sequencing, of which 25% had gray results. CONCLUSION: Results classified as ‘HPV-other’ type by the PANArray HPV test, or gray zone results by HC2 (RLU/CO ratio level 1–10) should be carefully interpreted using comprehensive clinical information.
Subject(s)
Female , Humans , Atypical Squamous Cells of the Cervix , Human papillomavirus 16 , Oligonucleotide Array Sequence Analysis , PapillomaviridaeABSTRACT
Resumen En la actualidad hay suficiente evidencia científica que relaciona directamente la adquisición, exposición y prevalencia del virus del papiloma humano (VPH) con el cáncer del cuello de uterino. Por ello, el artículo aborda el VPH en la mujer teniendo en cuenta la naprotecnología, que permite conjugar evidencia científica y planteamientos éticos. Se busca que se tengan en cuenta tanto el aspecto biológico de la sexualidad como la capacidad de hacerse persona en su núcleo sexual. De ahí que se analicen programas dirigidos a la educación sexual, basados tanto en la prevención sanitaria como en la antropología de la sexualidad, y apoyados por las instituciones familiares, que han demostrado una mejor acogida ante los riesgos de las enfermedades de transmisión sexual, y entre ellas la infección por el VPH.
Abstract There currently is sufficient scientific evidence directly linking acquisition, exposure and prevalence of the human papillomavirus (HPV) to cervical cancer. The article addresses HPV in women by taking NaProTechnology into account, which makes it possible to combine scientific evidence with ethical approaches. It looks at both the biological aspect of sexuality and the ability to become a person within one's sexual nucleus. There is an analysis of sex education programs based on preventive health and on the anthropology of sexuality, and supported by family institutions, that have demonstrated better acceptance in dealing with the risk of sexually transmitted diseases, including HPV infection.
Resumo Na atualidade, há suficiente evidência científica que relaciona diretamente aquisição, exposição e prevalência do Papilomavírus Humano (HPV) com o câncer do colo do útero. Por isso, este artigo aborda o HPV na mulher considerando a naprotecnologia, que permite conjugar evidência científica e proposições éticas. Procura-se que sejam levados em conta tanto o aspecto biológico da sexualidade quanto a capacidade de se fazer pessoa em seu núcleo sexual. A partir disso, são analisados programas dirigidos à educação sexual, baseados tanto na prevenção sanitária quanto na antropologia da sexualidade, e apoiados pelas instituições familiares, que têm demonstrado uma melhor acolhida diante dos riscos das infecções de transmissão sexual e, entre elas, a infecção pelo HPV.
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Humans , Sexually Transmitted Diseases , Uterine Cervical Neoplasms , Sexuality , Carcinogenesis , GenitaliaABSTRACT
@#Laryngeal SCCA usually presents with hoarseness when the glottis is involved, dysphagia if the supraglottis is involved, and difficulty of breathing and stridor in subglottic invovlement. A neck mass as an initial presentation of laryngeal carcinoma is commonly linked to the involvement of the supraglottis due to its rich lymphatic drainage. About 70% of supraglottic tumours present with advanced disease (stages III-IV),1 while 75% of glottic tumours present with localized disease (stages I-II).1 Smoking and alcohol consumption are considered highly significant etiologic factors but evidence has suggested a possible role for human papilloma virus (HPV) infection, ras oncogene activation, and gastroesophageal reflux as well.2 To the best of our knowledge, laryngeal squamous cell carcinoma has not been associated with herpes simplex virus (HSV). We report a case of laryngeal squamous cell carcinoma with an unusual presentation and peculiar histopathology, and discuss its potential association with herpes simplex virus.
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Humans , Male , Aged , Carcinoma , Larynx , Glottis , Men , SimplexvirusABSTRACT
Objective:To discuss the curative effect and security of recombinant human interferon α-2b vaginal effervescent capsules combined with matrine gels in the treatment of cervical human papilloma virus ( HPV) subclinical infection. Methods:Totally 74 cases of patients with cervical HPV subclinical infection were selected and divided into the observation group (n = 37) and the control group (n = 37) at random. The patients in the observation group were given recombinant human interferon α-2b vaginal effervescent capsules combined with matrine gels, while the patients in the control group were only given recombinant human interferon α-2b vaginal effervescent capsules for 3 treatment courses. The clinical curative effect and adverse reactions in the two groups after the medical treatment were observed, and the clinical curative effect in the 6-month follow-up after the medical treatment was observed and compared. Results:After the 3-month treatment, the total clinical efficiency in the observation group was much higher than that in the control group (P 0. 05). Six-month follow-up was performed and the total clinical efficiency in the observation group was much higher than that in the control group (P < 0. 05). Conclusion:Recombinant human interferon α-2b vaginal effervescent capsules combined with matrine gels have reliable curative effect on cervical HPV subclinical infection with high security and reliable long-term follow-up efficacy, which is benefit for the reoccurrence control of the disease.
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Introducción: el cáncer cérvicouterino (CCU) es la segunda neoplasia maligna más frecuente en mujeres. Objetivo: determinar el comportamiento de las lesiones intraepiteliales cervicales de alto grado. Métodos: se realizó una investigación cuantitativa y cualitativa de tipo descriptivo transversal de las pacientes atendidas en la consulta de patología de cuello del Hospital Universitario "Mártires del 9 de Abril" de Sagua la Grande, en el período comprendido entre enero y diciembre de 2011. El universo estuvo constituido por 158 mujeres que asistieron a consulta con citología alterada, la muestra fue de 70 mujeres con diagnóstico de lesión de alto grado. Se revisaron las historias clínicas, tarjetas de citodiagnóstico y se entrevistaron las pacientes, se confeccionó la base de datos. Resultados: la edad más frecuente fue 25-44 años. El 67,1 % inició su vida sexual entre los 16 y 20 años. Existió una relación directa entre la infección por el VPH y la aparición de las lesiones intraepiteliales cervicales, dado que en 53 pacientes (72,6 %) existió el hallazgo citológico del VPH. Hubo una correspondencia adecuada entre los resultados de la biopsia por ponchamiento y la biopsia por cono. En cuanto al tratamiento definitivo, a 53 pacientes se les realizó cono por bisturí frío y solo a 9 histerectomía total. Conclusiones: una de cada 10 pacientes afectada por una lesión de alto riesgo es menor de 25 años. A mayor tiempo transcurrido desde el inicio de las relaciones sexuales se incrementa la aparición de lesiones de alto grado.
Introduction: cervical cancer (CCU) is the second most common malignancy in women. Objective: to determine the behavior of high grade cervical intraepithelial lesions. Methods: a quantitative and qualitative cross descriptive study was conducted in patients treated in the cervix pathology service at the University Hospital Mártires del 9 de Abril , Sagua la Grande, from January to December 2011. The universe of our study consisted of 158 women who were assisted at consultation with abnormal cytology; the sample was 70 women diagnosed with high-grade lesion. We reviewed the medical records, greeting cytodiagnosis and interviewed the patients. Data were entered in a database. Results: the most common age was 25-44 years. 67.1 % started their sexual life between 16-20 years old. There was a direct relationship between HPV infection and the development of cervical intraepithelial lesions, since 53 patients (72.6 %) showed HPV cytologic finding. There was a suitable correspondence between the results of punch biopsy and cone biopsy. As for the definitive treatment, 53 patients underwent cold blade cone and only 9 underwent total hysterectomy. Conclusions: one in 10 patients affected by high-risk injury is younger than 25 years. A longer time since the onset of sexual intercourse increases the appearance of high-grade lesions.
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Se presenta el proceso de caracterización implementado para la obtención de descriptores visuales que representan el contenido visual de imágenes digitales de biopsias de cuello uterino infectadas con el Virus del Papiloma Humano (VPH), en las que se capturan tejidos con lesiones conocidas como Condiloma Plano Viral. A partir de la construcción de una base de datos de imágenes de biopsias de cuello uterino y el análisis e implementación de técnicas de filtrado que resaltan la información relacionada a las texturas contenidas en los tejidos que captura cada imagen y de técnicas de extracción de características que describen el contenido de las imágenes; se propone un conjunto de características que describen el contenido de las imágenes a partir de modificaciones propias de la Transformada Discreta de Wavelets y el cálculo de la Matriz de Coocurrencia, donde este conjunto de características propuesto proporcionó un porcentaje promedio de recuperación del 80% en imágenes microscópicas de cuello uterino infectadas con el VPH, sobre las cuales no se conocen sistemas CBIR desarrollados. Finalmente, se determina el porcentaje de recuperación promedio a partir del uso de métricas de similaridad basadas en la norma LP.
The purpose of this work is to report the characterization process implemented to obtain visual descriptors representing visual content of digital images of cervical biopsies infected with Human Papilloma Virus (HPV). Positive biopsies with infected tissues present lesions known as Condyloma Plano Viral. A database of images of cervical biopsies was constructed in addition to the implementation of techniques that enhance the texture information and describe the content of images. This work proposed a set of features to describe the content of images from custom modifications of Discrete Wavelet Transform and the calculation of the Co-occurrence Matrix. This proposed feature set provided an average recovery rate of 80% in microscopic images of the cervix infected with HPV, from which CBIR systems have not been developed. Finally, this work determines the average recovery rate from the use of similarity metrics based on the standard LP.
Neste trabalho é apresentado o processo implementado de caracterização para a obtenção de descrições visuais que representam o conteúdo visual de imagens digitais de biópsias cervicais infectadas com Papilomavírus Humano (HPV), capturadas em lesões de tecidos conhecidas como Condiloma Plano Viral. A partir da construção de uma base de dados de imagens de biópsias do colo uterino, análise e implementação de técnicas de filtragem de características que descrevem o conteúdo das imagems, propõe-se um conjunto de características que descrevem o conteúdo das imagens a partir de modificações próprias da Transformada Discreta de Wavelets e o cálculo da Matriz de co-ocorrência, onde o conjunto de características propostas resultou numa porcentagem média de 80% de recuperação nas imagens microscópicas de colo uterino infectado com o VPH, sobre as quais não se percebe o desenvolvimento dos sistemas CBIR. Finalmente, a taxa de recuperação média foi determinada a partir da utilização de métricas de similaridade com base na indicação de LP.
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Oral squamous cell carcinoma is the sixth most common malignancy worldwide. In Bangladesh, it comprises 20% of the whole body malignancies. Several studies found that 15% to 25% of oropharyngeal cancer cases are associated with human papilloma virus (HPV). This study is done to find the association of human papilloma virus subtypes, particularly HPV type 16 and HPV type 18, with the oral squamous cell carcinoma in Bangladeshi patients. In total, 34 diagnosed patients of oral squamous cell carcinoma were included in the study. Extracted DNA from the cancerous tissues was checked for PCR reaction to detect the subtypes of human papilloma virus. Data of the present study suggest that oral squamous cell carcinoma are almost absent in Bangladeshi patients with human papilloma virus, particularly HPV 16 and 18.
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Objective To investigate the expression and clinical significance of p63, CD_(44v6) and human papilloma virus (HPV) 16/18 in carcinoma of cervix and precancerous lesion and to study their role in the pathogenesis of the infiltrative carcinoma of the cervix(ICC). Methods 145 patients were selected, among whom were 60 cases of ICC, 55 cases of cervical intraepithelial neoplasia (CIN) and 30 cases of normal cervical epidermis (NCE). Immunohistochemistry streptavidin peroxidase (SP) was used to detect the expressions of p63 and CD_(44v6) protein and hybridization in situ was used to measure HPV 16/18 gene on tissue microarray. Results HPV 16/18, p63 and CD_(44v6) in ICC were significantly higher than those in CIN and NCE groups (P < 0.05). The expressions of HPV 16/18 gene in CIN I 、CIN II and CIN III were 27.3 % , 43.8 %, 70.6 % respectively. p63 protein was mainly expressed in squamous cell carcinoma, but not in adenocarcinoma. p63-positive rate was related to the grade of squamous cell carcinoma and clinical stage. CD_(44v6) positive rate was related to the pathological grade and clinical stage. Moreover, the positive rate of lymph node metastasis was significantly higher than the cases without metastasis (P <0.05). The expression of HPV16/18 was positively correlated with that of p63 in ISCC (P <0.05, Cp =0.49). Conclusion HPV16/18 possibly participate in the pathogenesis and progress of cervical squamous carcinoma. p63, as the cancer gene, may participate in the occurrence and development of cervical cancer caused by HPV 16/18. p63 could be a differentiation indicator of cervical squamous carcinoma, and it could be one of the markers which would be differentiated into squamous cell in malignant tumour. CD_(44v6) could be used as one of the indicators of distant metastasis of cervical cancer.
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BACKGROUND: Infection with human papilloma virus (HPV) is the main cause of cervical cancer, and HPV genotyping is of increasing importance for determining clinical course and management of the disease based on the HPV genotypes. Here, we established a novel matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS) assay, termed restriction fragment mass polymorphism (RFMP) that is suitable for genotyping multiple HPV in an accurate and high-throughput manner. We evaluated the performance of the RFMP assay in HPV genotyping by comparing the results with those of direct or clonal sequencing and hybrid capture (HC) assays. METHODS: The study population consisted of 50 patients with histologically confirmed cervical lesions and a positive test for HPV DNA. HPV genotyping was performed with RFMP, sequencing, and HC assays. The assigned genotypes and risk groups were compared among the methods. RESULTS: Concordance rates in the genotype level between RFMP vs sequencing, sequencing vs HC, and HC vs RFMP were 98% (49/50), 88% (44/50), and 88% (44/50), respectivley. Especially, RFMP and sequencing were 100% concordant when assigned high-risk group was considered identical in 1 case of mixed genotypes identified only in RFMP. The observed discrepancy between HC and the other two methods is due to the assignment of six cases of low, intermediate, or unassigned risk genotypes as high-risk group in HC method. CONCLUSIONS: RFMP, sequencing, and HC assays were highly concordant with each other in HPV genotyping. Compared to sequencing assay, RFMP assay is found to be advantageous in detecting mixed genotype infections. The accuracy and amenability to high-throughput analysis should make the RFMP assay suitable for reliable screening of HPV genotypes in clinical laboratories.
Subject(s)
Female , Humans , Genotype , Nucleic Acid Hybridization/methods , Papillomaviridae/classification , Papillomavirus Infections/diagnosis , Polymorphism, Restriction Fragment Length , Sequence Analysis, DNA/methods , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Uterine Cervical Neoplasms/diagnosisABSTRACT
OBJECTIVE To investigate the diagnostic effect of cervical cytology test combined with high-risk human papilloma virus detection in the diagnotic and screening tests for cervical cancer and its precancerous lesion.METHODS To screen 590 women by conventional Papanicolaou smear test combined with oncogenic human papilloma virus DNA testing,selecting the abnormalities and high-risks in clinic,and using vaginoscopical biopsy or cervical canals scraping.We diagnosed patients according to histopathology.RESULTS From 590 patients there were no high risk changes when cytology and human papilloma virus(HPV) were negative;the rate of ≥cervical intraepithelial neoplasia(CIN) Ⅱ was 10.1% when cytology(-) and HPV(+);the rate of ≥CIN Ⅱ was 39.39% when cytology(+) and HPV(+);and the rate of ≥CIN Ⅱ was 12.3% when cytology(+) and HPV(-).There were no CIN Ⅲ or even worse.CONCLUSIONS The way of cytology combined with high risk HPV test is accurate,swift and sensitive,it can be used into screening cervical cancer and its precancerous lesion,futhermore,the screening interval can be lengthened if the results are negative.
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Objective To explore the relation ship between the high-risk human papillomavirus(HPV) and prognosis of ovarian carcinomas.Methods In situ hybridization technique was used to examine HPV16/18 DNA in 32 cases of benign ovarian epithelial tumors,25 cases of borderline tumors and 45 cases of ovarian carcinoma.Immunohistochemistry was used to examine the expression of VEGF and ki-67 protein,which compared to usual ovarian tissues. Results (1)The positive rate of HPV16/18 was significantly different between ovarian carcinoma and normal epithelial ovarian tissues or benign epithelial ovarian tumors(P
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The squamous cell carcinoma of the uterine cervix is the most common malignant tumor among women in Korea. Since 1976, when a research result that human papillomavirus(HPV) infection played some role in the carcinogenesis of the uterine cervical carcinoma had been published, numerous reports supporting the result have been released. Among the types of the HPV, type 16 and type 18 are classified as high risk types because they are frequently found in cervical lesions with high grade dysplasia and invasive carcinomas. However, it is impossible to ascertain by host histologic or cellular changes which type of HPV is infected. The HPV genome is composed of six open reading frames (ORF' s) named as E1, E2, E4, E5, E6 and E7 in the early region. Among these oncoproteins HPV E6/E7 have been strongly suggested to be important in carcinogenesis. When HPV infects the epithelial cells, it promotes cellular proliferation. The cellular proliferation can be evaluated by immunohistochemistry with the antibodies for proliferating cell nuclear antigen(PCNA) and Ki-67. Because PCNA has long half-life, and can be detected 48 hours after completion of mitosis, an estimation of proliferating cells by PCNA could be inaccurate. The expression of Ki-67 antigen is more correct than PCNA for the evaluation of proliferation cells due to its short half-life and rapid degradation after completion of the mitosis. In this study, immunohistochemical staining was conducted to determine the rate of expression of HPV E6, E7 and Ki-67, correlation with relationship in carcinoma in situ and invasive uterine cervical cancer. Fifty cases of carcinoma of in situ(CIS) and invasive carcinoma were immunohistochemically stained, and the results obtained were as follows: 1) E6 protein of HPV type 16/18 was expressed in 5 of 14 cases(35.7%) of carcinoma in situ, in 3 of 7 cases of microinvasive carcinoma (42.8%) and in 12 of 20 cases of invasive carcinoma(60%) but there was no significant difference in expression between the carcinoma in situ and invasive cancer group (p=0.138). 2) E7 protein of HPV type 16/18 was expressed in 10 of 14 cases(71.4%) of carcinoma in situ, in 6 of 7 cases of microinvasive carcinoma (85.7%), and in 18 of 20 cases of invasive carcinoma(90%) but there was no significant difference in expression between the carcinoma in situ and invasive cancer group (p=0.138). 3) The cell fraction expressing Ki-67 was expressed in 5 of 14 cases(35.7%) of carcinoma in situ, in 5 of 7 cases of microinvasive carcinoma (71.4%), and in 18 of 20 cases of invasive carcinoma.(90%) The cell fraction expressing Ki-67 increased according to the progress of cervical cancer. 4) There was no statistical significance between HPV type 16/18 E6 protein and the cell fraction expressing Ki-67(p=0.09). 5) There was no statistical significance between HPV type 16/18 E7 protein and the cell fraction expressing Ki-67(p=0.17). The above results suggest that the cell fraction expressing Ki-67 increases according to the invasiveness of cervical cancer and E6/E7 protein seem to play a role in the progression of cervical cancer. However we were not able to reveal a relation between E6/E7 protein and the cell fraction expressing Ki-67 in progress of cervical carcinoma, and it is recommended that further studies should be undertaken.
Subject(s)
Female , Humans , Antibodies , Carcinogenesis , Carcinoma in Situ , Carcinoma, Squamous Cell , Cell Proliferation , Cervix Uteri , Epithelial Cells , Genome , Half-Life , Immunohistochemistry , Ki-67 Antigen , Korea , Mitosis , Oncogene Proteins , Open Reading Frames , Proliferating Cell Nuclear Antigen , Uterine Cervical NeoplasmsABSTRACT
OBJECTIVE To explore the genotype and distribution of humam papilloma viruses(HPV) among rural women with cervical lesion.METHODS The cervical exfoliated cell specimens were collected and divided into two groups,the experimental group with 340 rural women finally diagnosed as cervical intra-epithelial neoplasm(CIN) or higher grade pathological changes in healthy examination,and the health control group with 230 rural women randomly selected from the crowd taken healthy examination.DNA was extracted and the genotypes of HPV-DNA were monitored by traditional nested PCR,flow-through hybridization and gene chip technique.RESULTS One-hundred and ninety-five cases(57.4%) in experimental group and the 58 cases(25.2%) in control group were confirmed to be HPV-DNA positive.There was significant difference between the two groups(P