ABSTRACT
Objective: To establish a method for the determination of luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin by high performance liquid chromatography (HPLC) method, and the determination of luteoloside, apigenin-7-O-β-D- glucosidase, and luteolin in Humulus scandens female and male plants from different origins and different harvest months in Hebei province. Methods: HPLC method was used for simultaneous determination of luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin. System suitability conditions were as follows: column: Agilent 5 TC-C18 (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-0.2% phosphoric acid; flow rate: 1.0 mL/min; detection wavelength: 340 nm; column temperature: 25 ℃; injection volume: 10 μL. Results: The linear ranges were 4.640-74.24 μg/mL of luteoloside (r = 0.999 2), 1.510-30.20 μg/mL of apigenin-7-O-β-D-glucosidase (r = 0.999 9), and 0.796 3-12.74 μg/mL of luteolin. RSD of precision, stability, and repeatabillity tests were ≤ 1.99%. The average recoveries were 102.69% (RSD = 1.64%, n = 9), 100.49% (RSD = 1.93%, n = 9), and 100.39% (RSD = 1.65%, n = 9). Conclusion: The method is simple, reproducible, and accurate, and reliable measurement results can be used for simultaneous determination of luteoloside, apigenin-7-O-β-D- glucosidase, and luteolin in H. scandens female and male plants from different origins and different harvest months in Hebei province. There is greater difference between luteoloside, apigenin-7-O-β-D-glucosidase, and luteolin in H. scandens female and male plants from different origins and different harvest months in Hebei province.
ABSTRACT
Objective: To study the chemical constituents from the roots and stems of Humulus scandens. Methods: The chromatographies on silica gel column, reverse phase column, and semi-preparative HPLC were used to isolate and purify the constituents and the structures of the compounds were elucidated on the basis of physicochemical properties and spectral data. Results: Ten compounds were obtained from the ethyl acetate fraction of methanol extract in the roots and stems of H. scandens and identitied as N-p-coumaroyltyramine (1), N-trans-feruloyltyramine (2), cosmosiin (3), 3, 3'-dimethoxy-4, 8'-oxyneoligna-9, 4', 7', 9'-tetraol (4), 3-hydroxy-5, 6-epoxy-7-megastigmen-9-one (5), 4, 5-dihydroblumenol (6), scopoletin (7), citruusin C (8), 2-phenylethyl-O-β-D-glucopyranoside (9), and 3-oxo-α-ionol-β-D-glucopyranoside (10). Conclusion: Compounds 2, 4-6, and 8-10 are all obtained from H. scandens for the first time, and compounds 2, 4-6, 9, and 10 are firstly isolated from the plants of this genus. Compounds 2, 4, 7, and 9 show remarkable scavenging activity toward DPPH radical with IC50 values of 0.01, 3.36, 10.55, and 18.15 μg/mL, respectively. The scientific evidence is provided for the development of anti-oxidant and anti-aging aspects of the drugs.
ABSTRACT
Objective: To study the chemical constituents of Humulus scandens. Methods: The compounds were isolated and repeatedly purified by silica gel, TLC, and Sephadex LH-20 column chromatography, and their structures were elucidated on the basis of physicochemical constants and spectral analysis. Results: Eleven compounds were obtained and elucidated as (24R)-stigmast-7, 22(E)-dien-3β- ol (1), daucesterol (2), stigmast-3, 6-dione (3), epidioxyergosta-6, 22-dine-3β-ol (4), stigmasterol (5), soya-erebroside II (6), oleanolic acid (7), bis-(2-ethylhexyl) phthalate (8), scopoletin (9), betulimicaciol (10), and soya-erebroside I (11). Conclusion: The compounds 1, 3, 4 and 6-11 are isolated from the plants of Humulus Linn. for the first time.