Component analysis of nucleosides, amino acids, and mineral elements in pollen of Gingko biloba / 中草药

Objective: To analyze the nutritional ingredient in the pollen of Gingko biloba and provide the theoretical basis for the comprehensive utilization of pollen of G. biloba. Methods: The nucleosides and dissociative amino acids in the pollen of G. biloba were detected by UPLC-TQM. The content of nucleosides in pollen of G. biloba was calculated with the help of UPLC-TQ/MS, and the mineral elements in the pollen of G. biloba were determined by ICP-AES. Results: In pollen of G. biloba there were 24 abundant dissociative amino acids accounting for 62.316 mg/g totally. In this number, dissociative essential amino acids accounted for 6.23 mg/g with the highest content of 17.11 mg/g aspartic acid (ASP). Meanwhile, a small quantity of taurine, approximately 0.24 mg/g, has been tested out. Furthermore, there were 16 kinds of nucleosides in the pollen of G. biloba. Among those nucleosides, guanosine made up for the highest percent with 251.99 μg/g while other nucleosides accounted for a low percent. In pollen of G. biloba, K is the most abundant with 26.301 mg/g, followed by Ca with 7.076 mg/g, Mg with 5.361 mg/g, and Fe with 2.545 mg/g. As to the heavy mental elements, as and Cd were not found in it. Conclusion: From above analysis, pollen of G. biloba can provide the theoretical basis for the development of health-care food with pollen of G. biloba.

Differentiation of different brands of alcoholic beverages by trace element profile: A pilot study.

A pilot study was conducted to differentiate various brands of alcoholic beverages commonly sold in Hyderabad city (Andhra Pradesh, India). Various brands of liquor were scanned up to parts per billion (PPB) level for more than 72 elements using the following method – Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES). The results obtained showed significant difference in the elemental profiles of various brands of liquor. Absence of some elements such as copper showed marker parameter for the liquor brands. This survey shows that trace elemental profiling could be a reliable method for differentiating various brands of liquor.

Ingredients and cytotoxicity of MTA and 3 kinds of Portland cements / 대한치과보존학회지

The aim of this study was to compare the compositions and cytotoxicity of white ProRoot MTA (white mineral trioxide aggregate) and 3 kinds of Portland cements. The elements, simple oxides and phase compositions of white MTA (WMTA), gray Portland cement (GPC), white Portland cement (WPC) and fast setting cement (FSC) were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES), X-ray fluorescence spectrometry (XRF) and X-ray diffractometry (XRD). Agar diffusion test was carried out to evaluate the cytotoxicity of WMTA and 3 kinds of Portland cements. The results showed that WMTA and WPC contained far less magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) than GPC and FSC. FSC contained far more aluminum oxide (Al2O3) than WMTA, GPC, and WPC. WMTA, GPC, WPC and FSC were composed of main phases, such as tricalcicium silicate (3CaO.SiO2), dicalcium silicate (2CaO.SiO2), tricalcium aluminate (3CaO.Al2O3), and tetracalcium aluminoferrite (4CaO.Al2O3.Fe2O3). The significance of the differences in cellular response between WMTA, GPC, WPC and FSC was statistically analyzed by Kruskal-Wallis Exact test with Bonferroni's correction. The result showed no statistically significant difference (p > 0.05). WMTA, GPC, WPC and FSC showed similar compositions. However there were notable differences in the content of minor elements, such as aluminum (Al), magnesium, iron, manganese, and zinc. These differences might influence the physical properties of cements.

Analysis of Manganese Content in Frequently Consumed Foods by Koreans

Using ICP-AES, we analyzed manganese content in 366 foods consumed frequently by Koreans. For the analysis, it was included a total of 366 foods such as 51 kinds of grains, 7 kinds of potatoes and starches, 7 kinds of sugars and sweeteners, 12 kinds of legumes, 11 kinds of nuts and seeds, 68 kinds of vegetables, 7 kinds of mushrooms, 33 kinds of fruits, 13 kinds of meats, 4 kinds of eggs, 48 kinds of fishes and shellfishes, 7 kinds of seaweeds, 16 kinds of milks, 8 kinds of oils and fats, 27 kinds of beverages, 34 kinds of seasonings, 13 kinds of processed foods and others. Among the grains, starches and sugars, manganese content of rice was 0.745 mg/100 g. As for legumes, the content of manganese in soybean milk was 0.033 mg/100 g and in black beans was 4.075 mg/100 g. In nuts and seeds, the content of manganese in gingko nuts was 0.268 mg/100 g while that in pine nuts was 8.872 mg/100 g. Among the vegetables, manganese contents were 0.061 mg/100 g in cherry tomato and 14.017 mg/100 g in ginger. In mushrooms, the highest manganese content was displayed in ear mushroom at 10.382 mg/100 g. Dried jujube and shrimp were found to be the fruits and fishes with high manganese contents at 2.985 mg/100 g and 3.512 mg/100 g, respectively. Among dairy foods, oils and beverages, manganese content was the highest in instant coffee powder at 2.577 mg/100 g. Seasonings and processed foods posted 0.010 mg/100 g in Sagolgomtang, instant soup and 23.846 mg/100 g in pepper. In a furture, more various food for manganese content needs to be analyzed and a reliable food database should be compiled from the findings of researches in order to estimate manganese consumption accurately.

Studies on Contents of Heavy Metals in Niuhuang Jiedu Tables / 浙江中医药大学学报

[Objective]To compare the contents of heavy metals in 6 types of Niuhuang jiedu tables.[Methods]The content of heavy metals in 6 types of Niuhuang jiedu tables before or after passed through metabolically man-made stomach liquid was detected by inductively coupled plasma atomic eminssion spectrometry(ICP-AES).[Results]These contents of heavy metals in 6 drugs were more than the order in chinese standards.But the contents of acid-soluble heavy metals were much less than or partially accorded with total heavy metals in the same batch of sample.[Conclusion]The content of acid-soluble heavy metals may be taken as one of the items of quality control for the Niuhuang jiedu tablets and traditional Chinese medicine patent prescription.

Determination of the Content of Zn in Shenningsan Capsules by ICP-AES Capsules by Atomic Absorption Spectrometry / 中国中医药信息杂志

Objective To establish an ICP-AES method for determination of the content of Zn in Shenningsan Capsules. Methods The sample was digested with HNO3-HClO4, and determined by the ICP-AES method. The determined wavelength was at 213.8 nm, the electric current of the light was set at 10.0 mA, the slit-width was 0.5 nm, the flow rate of acetylerce was 2.0 L/min and the flow rate of air was 9.4 L/min, the hight of burning was 7.5 mm. Results The ion concentration of Zn had the good linear range of 0.2～1.6 ?g/mL, Y=0.054X+0.001 1 (r2=0.999 3). The average recovery of the content of Zn in Shenningsan Capsules was 99.56% with RSD of 2.00%. The content of Zn in Shenningsan Capsules was 68.6 ?g/g. Conclusion The method is simple and reliable. It can be used effectively for the quality control of Shenningsan Capsules.

Determination of reference concentrations of strontium in urine by inductively coupled plasma atomic emission spectrometry

<p><b>OBJECTIVE</b>The aim of this study was to establish reference concentrations of urinary strontium by inductively coupled plasma atomic emission spectrometry (ICP-AES).</p><p><b>METHODS</b>For the determination of strontium, urine samples were collected from healthy Japanese (n=146; 115 males, 31 females; mean age, 33±9 years; age range, 18 to 58 years). The urine samples stored at or below -20°C were thawed with incubation at 40°C for 30 min and sediments were dissolved by vigorous shakings. Then, the samples were centrifuged at 3000 g for 5 min, and the supernatant was directly aspired into a P-5200-3600/1200 ICP-AES system from Hitachi Ltd., Tokyo, Japan.</p><p><b>RESULTS</b>A steeper increase in the S/N ratio and a good effective linearity of the calibration line was obtained at 407.771 nm in the range of 0-300 μg/L strontium standard solution. Urine samples having the same background signal as that of 18 MΩ cm ultrapure blank water, a good correspondence of the single peak pattern of the spectra, accuracy and precision of spike recovery were also confirmed. Urinary strontium concentrations showed a log-normal distribution and a geometric mean concentration of 143.9 μg/L, with 5-95% confidential interval of 40.9-505.8 μg/L.</p><p><b>CONCLUSION</b>The results of this study will be useful as guidelines for the biological monitoring of strontium in normal subjects and in individuals therapeutically or environmentally exposed to strontium.</p>

Determination of Reference Concentrations of Strontium in Urine by Inductively Coupled Plasma Atomic Emission Spectrometry

Objective: The aim of this study was to establish reference concentrations of urinary strontium by inductively coupled plasma atomic emission spectrometry (ICP-AES). Methods: For the determination of strontium, urine samples were collected from healthy Japanese (n=146; 115 males, 31 females; mean age, 33±9 years; age range, 18 to 58 years). The urine samples stored at or below −20°C were thawed with incubation at 40°C for 30 min and sediments were dissolved by vigorous shakings. Then, the samples were centrifuged at 3000 g for 5 min, and the supernatant was directly aspired into a P-5200-3600/1200 ICP-AES system from Hitachi Ltd., Tokyo, Japan. Results: A steeper increase in the S/N ratio and a good effective linearity of the calibration line was obtained at 407.771 nm in the range of 0–300 μg/L strontium standard solution. Urine samples having the same background signal as that of 18 MΩ cm ultrapure blank water, a good correspondence of the single peak pattern of the spectra, accuracy and precision of spike recovery were also confirmed. Urinary strontium concentrations showed a log-normal distribution and a geometric mean concentration of 143.9 μg/L, with 5–95% confidential interval of 40.9–505.8 μg/L. Conclusion: The results of this study will be useful as guidelines for the biological monitoring of strontium in normal subjects and in individuals therapeutically or environmentally exposed to strontium.

Determination of blood boron by inductively coupled plasma atomic emission spectrometry and investigation of boron level in children's blood / 西安交通大学学报(医学版)

Objective To study inductively coupled plasma atomic emission spectrometry(ICP-AES) used to determine blood boron(B),and to investigate the level of B in blood of children in Nanjing.Methods ICP-AES was applied to determine the level of B in the blood of 1032 healthy children and 1364 children patients after using 1mol/L HNO_(3) to treat whole blood.Results Precision of this method was 1.60%-4.31%,and recovery ratio was 93.3%-98.9%.The level of B in blood of the 1032 healthy children was(41.8?16.7)?g/L and that in blood of the 1464 patients was(25.1-58.5)?g/L(77%).Conclusion Precision and recovery ratio determined by the method are high,so it can be used as a reference method to detect the level of B in blood.And it preliminarily determines the reference levels of blood B of children in Nanjing as ranging from 25.1-58.5?g/L(except children below 1 year old).

Determination of Trace Elements in Plumbago zeylanica / 中国药房

OBJECTIVE:To establish a determination method for trace elements of Plumbago zeylanica. METHODS:Induct- ively coupled plasma emission spectrum (ICP-AES) was used to determine seven trace elements of P. zeylanica, i.e. Cu, Zn,Fe,Ca,Mg,Mn and Sr simultaneously. Se was determined by atomic fluorescence (AFS) method. RESULTS: The RSD ranged from 0.78% to 3.01%(n=5),while the recovery was within 91.9%～105.8%. The result obtained was satisfactory. Se took a large proportion in P. zeylanica, followed by Cu,Zn,Fe,Ca,Mg,Mn,Sr.CONCLUSION: The method is brief, rapid and accurate for the determination of P. zeylanica. The study provide reference for future pharmacological study of P. zeylanica.

Avaliaçäo do teor de arsênio total em pescado de água doce / Evaluation of arsenic content in fish from freshwater

Foi determinado o teor de arsênio total em polpas e filés das seguintes espécies de peixes de água doce: Pacu (piaractus mesopotamicus), Matrinxä (Brycon lundi), Carpa (Cyprinius carpio), Tilápia (Oreochromus niloticus), Curimbatá (Prochilodus sp), Tambaqui (Colossoma maceropomum), Bagre Africano (Clarias sp), procedentes do centro de Pesquisa e Treinamento em Aquicultura, Pirassununga - Säo Paulo. A determinaçäo do elemento arsênio foi realizada por espectrometria de emissäo atômica com fonte de plasma de argônio induzido (ICP-AES) acoplado a um gerador de hidretos, nas amostras previamente mineralizadas por via seca com nitrato de magnésio. Os teores obtidos de arsênio (ug kg-1) para os filés e polpas foram, respectivamente: Pacu (49 e 8), Matrinxä (27 e 24), Carpa (66 e 209), Tilápia (37 e 277), Curimbatá (30 e 93), Tambaqui (42 e 24) e Bagre (43). Em todas as amostras foram encontrados níveis de arsênio inferiores ao recomendado pela legislaçäo brasileira. (AU)

The level of arsenic was determined in pulps and steak of the following freshwater Fish species: Pacu (Piaractus mesopotamicusi, Matrinxã (Brycon lundii, Carp (Cyprinius carpio], Tilápia tOreochromis niloticusi,Curimbatá tProchilodus sp),Tambaqui (Colossoma macropomumi, Bagre Africano (Clarias sp). The samples were obtained from the Center of Research and Training in Agriculture, Pirassununga, São Paulo.The determination of the arsenic elemento was erforrned By hydride generator-inductively coupled plasma atomic emission spectrometry (ICP-AES), after minera- lizing by drying with magnesium nitrate of the samples.The quantity obtained of arsenic in ug kg' from the fishes for steak and pulps respectively were: Pacu (49 e 8), Matrinxã (27 e 24), Carp (66 e 209), Tilápia (37 e 277), Curimbatá (30 e 93), Tambaqui (42 e 24) e Baqre (43). Arsenic levels below the ecommended Brazilian legislation were found in ali samples. (AU)

Study on inorganic elements in Corium Bufo bufo garagarizans Cantor / 中成药

Objective: Qualitative and quantitative determination of the contents of inorganic elements in Corium Bufo bufo gargarizans Cantor. Methods: Qualitative determination by atomic emission spectrometry and quantitative determination by ICP AES.Conclusion: The content of calcium is the highest in Corium Bufo bufo gargarizans Cantor.

THE RESEARCH ABOUT SERUM TRACE ELEMENTS OF 7-20 YEARS-OLD POPULATION IN SHANGHAI / 营养学报

Seventeen trace elements, including twelve essential trace element, and three normal elements in serum of 417 healthy students aged 7 to 20 in Shanghai were analysed. Except Se which was determined by catalytic pulse polarography, Zn, Cu, Fe, Mn, Cr, Mo, Si, Sn, Ni, V, Co, Cd, Pb, Al, Ti, Sr, Ca, Mg and P in serum were measured by ICP-AES. The result showed that the distribution curve of normal elements and Zn, Cu in serum was more concentrated than the other trace elements. There were significant positive correlation between serum Zn, Fe, Mn, Sr and age, and negative correlation between Cu, Cr, Se, Pb and age, respectively. The elements of males were the same as that of females in total population, but the majority of the elements in serum had sex difference in different age-groups. The serum Cr, Si, Pb contents were higher, and Cu, Fe, Mn, Sr were lower in the urban than in the rural adolescents.