ABSTRACT
OBJECTIVE: To study and exploit Chinese medicine Astragali Radix, the molecular markers that relates to the phenotypic traits on medicinal components of Astragali Radix and would be detected. METHODS: The genetic diversity of 43 Astragali Radix samples was analyzed with ISSR molecular marker technique and then the population genetic structure was studied through 13 selected markers. The association analysis between ISSR markers and 4 phenotypic traits of medicinal components were performed with GLM (general linear model) programs in Tassel 2.1. Certain genetic diversity was discovered among the 43 Astragali Radix samples. RESULTS: The genetic distance varied between 0.050 6 and 0.743 8, with an average of 0.274 1. Moreover, the cultivated Astragali Radix from Ningxia and Gansu province closely related to the wild Astragali Radix collected from Liupanshan town in Ningxia. On the other hand, No. 340 had the farthest relationship with other Astragali Radix. The content of polysaccharide, total saponins, total flavonoids, and Astragaloside IV ranged between 7.693-27.840 mg•g-1, 7.167-17.579 mg•g-1, 2.212-6.164 mg•g-1 and 6.070-107.920 μg•g-1, respectively. Meanwhile, linear regression analysis indicated that there was a significant positive correlation between the content of the total saponins and that of flavonoids (r=0.650 5,P=2.3×10-6<0.01), while the content of astragaloside had no significant correlation with that of polysaccharide, total saponins and total flavonoids. The population genetic structural analysis showed that the 43 samples were divided into 4 subgroups. There were total of 34 locus in 13 ISSR markers significantly associated (P<0.01) with the content of polysaccharide,total saponins, flavonoids and astragaloside , and the rate of explanation on the phenotype of related marker ranged from 8.14% to 51.39%. Among the locus, 15 were related with astragaloside content at interpretation rates above 30%, 1 with polysaccharide content an interpretation rate reached as high as 51.39% with high threshold (P<1×10-5). CONCLUSION: These results would provide supporting evidence for identification and protection of germplasm resources as well as molecular marker-assisted breeding.
ABSTRACT
Objective In order to provide some information for the domestication and protection of wild Anisodus tanguticus, therefore to analyze the genetic polymorphism and the relationship of A. tanguticus from Gansu and Qinghai province in China. Methods The amplifying products from 127 DNA based on ISSR makers technique were used to analyze the genetic diversity of 11 populations of wild A. tanguticus. The clustering analysis of the unweighted pair-group method with arithmetic average (UPGMA) and making Mantel test on geographic distance were carried out on the basis of genetic distance abtained, then a dendrogram was constructed. Results Total 131 alleles were amplified by using 10 ISSR primers. The number of alleles in per primer range was 11-15 with an average of 13.1, and 858 polymorphic loci were got finally. The average percentage of polymorphic loci (PPB) was 59.54%. The Nei’s gene diversity (H) and Shannon’s information index (I) were 0.220 4 and 0.325 4 respectively. The genetic distances range was 0.073 3-0.306 2, and Mantel test P = 0.002. Conclusion The genetic polymorphism among 11 populations of A. tanguticus is relatively abundant and there is a directly relationship between genetic distance and geographical distribution among them. There is a high polymorphism among A. tanguticus populations between Gansu and Qinghai and the plentiful genetic diversity of species can provide a reference for the protection and sustainable utilizing of wild A. tanguticus resources.
ABSTRACT
Objective: To analyze the genetic polymorphism and relationship of Sophora alopecuroides from Ningxia, Gansu, Qinghai, Xinjiang, and Inner Mongolian in China, and to offer some information for the domestication and protection of wild S. alopecuroides germplasms. Methods: ISSR primers were used to analyze the genetic diversity of 22 populations of S. alopecuroides. Cluster analysis of the unweighted pair-group method with arithmetic average (UPGMA) and principal component analysis (PCA) were carried out based on the molecular data obtained, and a dendrogram was constructed. Results: Totally 433 alleles were amplified using the 51 ISSR primers. The number of allels in per primer range was 5-12, with an average of 8.49. The average percentage of polymorphic loci (PPB) was 93.30%. The Nei's gene diversity (H) and Shannon's information index (I) were 0.3351 and 0.4998, respectively. The genetic distances range was 0.1736-0.6502. Conclusion: The genetic polymorphism among 22 populations of S. alopecuroide is relatively abundant and there are no direct relationship between genetic distance and geographical distribution in them.