Desmoplastic small round cell tumor of the ovary: A case report with a new modality of treatment and review of the literature

Abstract Objective Desmoplastic small round cell tumor (DSRCT) is a rare intraabdominal neoplasm that grows along serosal surfaces and is primarily found in young men. To Keywords date, only 16 cases of ovarian DSRCT have been previously reported in women in the English literature, and no large population-based studies on this topic exist. Case Report We report the case of a 19-year-old virgo with unremarkable past medical history, initially presented with abdominal fullness. After being treated with the optimal treatment modality (primary and secondary surgical debulking, unique chemotherapy, protocol and adjuvant radiotherapy), the patient has remained without tumor disease for 40 months. Conclusion Although the best therapy for patients with DSRCT has yet to be determined, combining complete surgical resection, adjuvant chemotherapy, and radiotherapy is required to prolong survival and to achieve proper quality of life.

Role of Ki 67 Labelling Index as an Adjunct to Histopathological Diagnosis for Grading of CNS Tumours

The Central Nervous System tumours are unique as they arise from specialized tissue. CNS tumours constitute a wide range of neoplasm that differs in their location, age distribution, extent of invasiveness, morphological features and tendency for progression. We wanted to evaluate the traditional morphological data with knowledge on the prognostication marker Ki 67 antibody in evaluating tumour grade and prognosis of CNS neoplasm.METHODSThis is a cross section study carried out between March 2015 and September 2016 in histopathology department of Dhiraj Hospital on 50 cases of CNS tumours. Immunolabelling of all biopsies was done by horse radish peroxidase technique using rabbit monoclonal antibody to Ki 67 (clone SP 6) (Thermo Scientific, USA). Ki 67 immune positive labelling index was obtained for each tumour and correlated with mitotic labelling index obtained by conventional morphological grading as per WHO 2007 classification.RESULTSIn our study of CNS tumours, all age groups were studied. The mean Ki 67 labelling index (LI) values +/-SD for WHO Grade I tumours were- meningiomas (10) 3.85 (+/1.97) %, schwannoma (3) -3.0 (+/-2.97) %, pituitary adenoma (1) 0.6, craniopharyngioma (1) -1.1% and ependymoma (6) 2.62 (+/-0.60) %. WHO Grade II tumours- atypical angiomatous meningioma (1) -2%, atypical mucinous meningioma (2) -6.15 (+/-1.06) %, gemistocytic astrocytoma (1) -12; pleomorphic xanthoastrocytoma (2) -4.3 (+/-0.99) %, astrocytoma grade ii (2) -3.3 (+/-0.71) %, oligodendroglioma grade ii (4) - 3.9 (+/-0.88) %. WHO grade III tumours- anaplastic astrocytomas (5) -6.82 (+/-2.17) %, anaplastic oligoastrocytoma grade iii (1) -10.8 %. who grade iv-glioblastomas (7) -18.44 (+/-3.97) %; medulloblastomas (1) 20%, metastatic tumour (3) -36 (+/- 22.16) %. In our study, the mean Ki 67 LI (± SD) values for grade II, III and IV glioma is as follows: 4.76 (+/-2.83) %, 7.48 (+/-2.53) % and 18.44 (3.97) % respectively.CONCLUSIONSThis study shows that Ki 67 LI serves as an essential clinical prognostic proliferation marker of particular importance in cases with lower grade histology of Grade II & Grade III astrocytomas, Grade II & Grade III oligodendrogliomas. Ki 67 LI is important in determining benign, atypical and malignant meningiomas, non-invasive and invasive pituitary adenomas.

Gastrointestinal Stromal Tumor (GIST)-Clinical Profile & Management of Patients Admitted To a Tertiary Care Hospital in Odisha

Gastrointestinal stromal tumor (GIST) is the commonest mesenchymal tumor of gastrointestinal tract. Gastrointestinal bleeding, obstruction, pain and abdominal lump are the common clinical manifestations. Local or segmental resection provides satisfactory results. Aim: Our aim was to report our experience of gastrointestinal stromal tumors (GISTs) during the last 2 years. Methods: Between January 2017 and June 2019, we performed surgery for 12 cases of GIST. Metastases, recurrence and survival data were collected in relation to age, history, clinical presentation, location, size, resection margins and cellular features. Results: Resection was completed in 11 cases. In one case definitive surgery was abandoned due to local invasion and metastasis. Three patients with high risk GIST were treated with imatinib mesylate. Conclusion: Non-radical surgery in the form of local or segmental resection is the standard surgical approach for GIST management. Pathological and biological features of the neoplasm represent the most important factors predicting the prognosis.

Gastrointestinal Stromal Tumor (GIST)-Clinical Profile & Management of Patients Admitted To a Tertiary Care Hospital in Odisha

Gastrointestinal stromal tumor (GIST) is the commonest mesenchymal tumor of gastrointestinal tract. Gastrointestinal bleeding, obstruction, pain and abdominal lump are the common clinical manifestations. Local or segmental resection provides satisfactory results. Aim: Our aim was to report our experience of gastrointestinal stromal tumors (GISTs) during the last 2 years. Methods: Between January 2017 and June 2019, we performed surgery for 12 cases of GIST. Metastases, recurrence and survival data were collected in relation to age, history, clinical presentation, location, size, resection margins and cellular features. Results: Resection was completed in 11 cases. In one case definitive surgery was abandoned due to local invasion and metastasis. Three patients with high risk GIST were treated with imatinib mesylate. Conclusion: Non-radical surgery in the form of local or segmental resection is the standard surgical approach for GIST management. Pathological and biological features of the neoplasm represent the most important factors predicting the prognosis.

Immunohistochemical profile and its association with clinicopathological parameters in carcinoma breast: a prospective study in central India

Background: Carcinoma breast is one of the most common malignancies of women in India. The current study was conducted with the objective of assessing estrogen receptor (ER), progesterone receptor (PR), Her-2/neu (human epidermal growth factor receptor-2) expression and Ki67 index of breast carcinomas and its correlation with histological grade, tumour size and lymph node metastasis.Methods: Forty-seven lumpectomy or modified mastectomy specimens diagnosed as Infiltrating duct carcinoma (IDC): NOS, were selected for panel of imuno histochemistry (IHC) markers on tissue microarray blocks prepared from each case.Results: Maximum of our patients belonged to premenopausal 24/47 (51%) and 20% to younger age group (<30 year). Tumour size of 2-5 cm was observed in maximum females 29 (61%); while 13(27%) had size >5.0cm. The majority of cases diagnosed as grade I (40%) and lymph node involvement was seen in 31/47 (65%). Molecular classification revealed 10 (21%) luminal A, 4 (8%) luminal B, 9 (19%) Her2/neu positive, while triple negative phenotype comprised of maximum 24 (51%) patients. Most of the Luminal group tumours were low grade (14/15); while majority of Her2/neu positive 7/9(77%) and triple negative tumours 19/24 (80%) belonged to higher grades.Conclusions: Breast carcinoma among our patient is characterized by a large percentage of triple negative phenotype that is less susceptible to hormonal therapy. The empirical treatment with tamoxifen should therefore be reconsidered as it would be less effective. Assessment of prognostic markers in breast carcinoma is strongly advocated in order to provide the best therapeutic options.

Expression of TNF-α and PCNA in human breast tissue injected with polyacrylamide hydrogel and clinical significance / 中华整形外科杂志

Objective@#To study the expression of TNF-α and PCNA in human breast tissue with polyacrylamide hydrogel(PAHG) injection, and to provide the initial theory basis for its prognosis and clinical treatment.@*Methods@#Immunohistochemistry was used to measure the expression of tumor necrosis factor α(TNF-α)and proliferating cell nuclear antigen (PCNA) in 20 normal breast tissues and 40 cases with PAHG injection, analysis was also done by HE staining.@*Results@#①HE staining showed that there were a large number of homogeneous amorphous gel-like injections under optical microscope. Around PAHG there were different degrees of fibrous tissue hyperplasia with or without fibrous degeneration and lots of inflammatory cells. Local foreign body giant cell reaction and ductal dilatation also can be seen around PAHG. ②The IA levels of TNF-α and PCNA in the experimental group were 3.9± 0.3 and 3.2 ± 0.2, the IA levels of TNF-α and PCNA in the control group were 1.0 ± 0.1 and 1.3 ± 0.3, the IA level in the experimental group was significantly higher than that in the control group (P=0.000). The difference was statistically significant. The positive rates of TNF-α and PCNA in the experimental group were 90% (36/40)and 85% (34/40)respectively; the positive rates of TNF-α and PCNA in the control group were 30% (6/20), 40% (8/20). The positive expression rate in the experimental group was significantly higher than that in the control group (P=0.000). The difference was statistically significant. TNF-α and PCNA has Pearson positive relevance in the changes(r=0.3, P=0.040), the difference was statistically significant.@*Conclusions@#TNF-α and PCNA are involved in the immunoreaction of mammalian breast tissue injected with polyacrylamide hydrogel, which may induce the aseptic inflammation, fibrous tissue hyperplasia and related issues through mutual influence.

Expression of MAGE-A9 in colorectal cancer and its clinical significance / 重庆医学

Objective To observe the expression change of melanoma-associated antigen(MAGE)-A9 in colorectal cancer (CRC)tissue and to explore its significance.Methods The samples in 23 cases of initially diagnosed CRC in the Affiliated Hospital of Nantong University from January 2006 to December 2008 were collected.The quantitative real-time(qRT)-PCR was adopted to detect MAGE-A9 mRNA expression in cancer tissue and corresponding paracancerous tissue.Its correlation with the clinicopatho-logical features and prognosis was analyzed.Results The positive rate of MAGE-A9 in CRC tissue was significantly higher than that in paracancerous normal tissue(P<0.05).MAGE-A9 protein expression in CRC was related to the clinicopathological features such as tumor differentiation degree(P=0.011),TNM stage(P=0.003),tumor infiltration depth(P=0.001)and lymph node me-tastasis(P=0.003).The survival analysis showed that the expression of MAGE-A9 was closely related to the prognosis of CRC pa-tients.Conclusion MAGE-A9 expression is increased in CRC tissue,suggesting the poor prognosis.

Clinical significance of Bcl-6 expression in young DLBCL patients with Myc+ or Bcl-2+Myc+ / 肿瘤

Objective: To investigate the expression of Bcl-6 in newly diagnosed and young patients with diffuse large B cell lymphoma (DLBCL), and to further explore its clinical significance in young DLBCL patients with Myc positive expression (Myc+) or Bcl-2 and Myc positive expressions (Bcl-2+Myc+). Methods: Immunohistochemical (IHC) staining was used to detect the expressions of Bcl-6, Myc and Bcl-2 in 116 newly diagnosed and young patients with DLBCL, and the complete clinical and pathological data were collected. The relationship between the expression levels of Bcl-6 protein and the prognosis of young DLBCL patients with Myc+ and Bcl-2+Myc+ was retrospectively analyzed. Results: There was no significantly correlation between the expression of Bcl-6 alone and the prognosis of young DLBCL patients (P > 0.05). However, for the DLBCL patients with Myc+, the prognosis in Bcl-6 negative (Bcl-6–) group was worse than that in Bcl-6 positive (Bcl-6+) group (P < 0.05). Furthermore, the multivariate COX regression analysis showed that Bcl-6–Myc+ expression was a significant independent factor of adverse prognosis except for the international prognostic index (IPI) (P < 0.05). Meanwhile, the prognosis of DLBLC patients with Bcl-6–Bcl-2+Myc+ was significantly worse than that of the patients with Bcl-6+Bcl-2+Myc+ (P < 0.05). Conclusion: Bcl-6 negative expression can increase the risk of poor prognosis in young DLBCL patients with Myc+ or Bcl-2+Myc+.

Mantle Cell Lymphoma-A Retrospective 2-Year Study

Materials and methods Retrospective study for period of 2 years was conducted. For this study, we reviewed bone marrow material along with nodal and extranodal tissues. There were 16 cases of mantle cell lymphoma (MCL). Each patient had an absolute lymphocyte count of more than 10 × 109/l at the time of initial evaluation at our institution. Giemsa stained peripheral blood and bone marrow aspirate smears were reviewed, along with haematoxylin-eosin-stained histologic sections of bone marrow aspiration and core biopsy specimens. The immuno-phenotype of the neoplastic cells supported the diagnosis of MCL. The clinical and pathological spectrum will be discussed. Immuno-histochemistry Immuno-histochemical staining for CD3, CD20, CD23, CD1O, KI67 CYCLIN D1 were performed on formalin-fixed, paraffin-embedded tissue sections of either bone marrow aspirate or core biopsy tissue sections in all 16 cases. Results There were 11 men and 5 women with a median age of 68 years (range, 40–74 years). Physical examination revealed splenomegaly in 15 out of 16 patients. Lymphadenopathy involving multiple sites was present in 10 patients. Conclusion MCL can exhibit a wide spectrum of morphologic findings. We suggest that cell size and chromatin characteristics are useful for dividing these cases into two groups: small cell and large/blastoid. The large/blastoid group predicts poorer prognosis and includes cases with large cells, many of which are nucleolated and resemble prolymphocytes, as well as blastoid cells that resemble lymphoblasts. In this study, a cut-off of at least 20% large/blastoid cells best predicted poorer survival.

Expression and Significance of Mcl-1 Gene in Hepatocellular Carcinoma / 现代检验医学杂志

Objective To investigate the expression and significance of myeloid cell leukemia-1 (Mcl-1 )gene and protein in Hepatocellular Carcinoma(HCC).Methods The expression of Mcl-1 was detected respectively by Reverse Transcription-Polymerase Chain Reaction (RT-PCR),Western blot and ENVISION immunohistochemistry in 20 HCC specimens,19 liver cirrhosis(LC)specimens,and 12 control ones.Their relationship with clinical and pathological characteristics of HCC was investigated.Results The expression of Mcl-1 mRNA in the control group,LC group and HCC group was 0.52±0.17, 3.46±1.7,3.65±2.93,respectively.The level in HCC and LC group was statistically different compared with the control group,respectively (t=7.925,5.334,P<0.05).The relative expression of Mcl-1 protein in LC group (0.51±0.35)and HCC group (0.75±0.36)were significantly higher than that in the control group (0.21±0.19)(t=5.526,6.355,P<0.05).The positive expression rate of Mcl-1 in HCC group was 55.00% (11/20),significantly higher than that in the con-trol group 33.33% (4/12)(t=7.835,P<0.05).The positive expression of Mcl-1 was related to tumor necrosis and TNM staging (χ2=4.201,P<0.05).Conclusion Mcl-1 gene and protein are differentially expressed in HCC,LC and the control, which may be involved in the occurrence,development and malignant transformation of HCC.

Plasma Epstein-Barr Virus (EBV) DNA as a Biomarker for EBV-associated Hodgkin lymphoma.

Objective: To assess plasma Epstein-Barr virus (EBV) DNA as a biomarker of tumour burden at diagnosis and during therapy in children with Hodgkin lymphoma. Design: Case-control study, with prospective follow-up of the Hodgkin lymphoma cohort (2007-2012). Setting: Pediatric Hematology Oncology unit of a tertiary care hospital in Delhi. Patients: Thirty children with Hodgkin lymphoma and 70 sex and age-matched controls (benign lymphadenopathy 19, non-lymphoid malignancy 29, Burkitt lymphoma 5, healthy children 17). Intervention: Positive EBV-staining on immunohistochemistry was defined as EBV-associated Hodgkin lymphoma. Plasma EBV real-time quantitative polymerase chain reaction (PCR) was tested at presentation, after first and last chemotherapy cycles, and on follow-up. Main outcome measures: Plasma EBV quantitative PCR was compared between cases and controls. Its kinetics was assessed during and after chemotherapy. Results: EBV quantitative PCR was positive in 19 (63%) Hodgkin lymphoma cases (range 500–430,000 copies/mL), with 87.5% accuracy (kappa=0.69) as compared with EBVimmunohistochemistry. Sensitivity and specificity of the quantitative PCR were 87.5% and 81.8%, respectively. Only boys showed positive EBV immunohistochemistry and/or quantitative- PCR positivity. All controls were quantitative-PCR negative. All quantitative-PCR positive cases with follow-up blood sample showed EBV clearance after the first cycle. A quantitative-PCR negative case in long-term remission became positive at relapse. EBV status did not influence survival. Conclusion: Plasma EBV-DNA, detectable in EBV-associated Hodgkin lymphoma, becomes undetectable early after initiating therapy. It can be used as a biomarker of treatment response in EBV-associated Hodgkin lymphoma.

Effects of fluoxetine on changes of GLT-1 in rat prefrontal cortex after chronic unpredictable stress / 中国药理学通报

Aim To investigate the effects of fluoxe-tine on the changes of of protein levels of GLT-1 in pre-frontal cortex in rat depression model, and to further explore the molecular mechanism of antidepressant ac-tion of fluoxetine. Methods Sixty male SD rats were randomly assigned into three groups: control group, chronic unpredictable stress ( CUS) group, and CUS+fluoxetine group. The rats of CUS group and CUS+flu-oxetine group were subjected to CUS for 2 sessions per day for 35 days. Then, the rats of the CUS+fluoxetine group were given fluoxetine for 28 days. Behavioral changes were assessed by the sucrose preference and open field tests. The GLT-1 protein levels in the pre-frontal cortex were detected by immunohistochemistry and Western blot analysis at the end of the fluoxetine treatment. Results ( 1 ) Compared with the control group,sucrose preference, total traveling distance, ve-locity and frequencies of rearing were reduced in the CUS group ( P < 0. 01 ) . These behavioral changes could be reversed after 28 day fluoxetine treatment. (2 ) Immunohistochemistry assay indicated weak im-munoreactivity for GLT-1 in the prefrontal cortex of CUS group ( versus the control rats: P <0. 01 ); the immunoreactivity for GLT-1 of the fluoxetine-treated rats was significantly up-regulated compared with the CUS group rats ( P<0. 01 ) . ( 3 ) Western blot analy-sis indicated significant reductions of GLT-1 in the pre-frontal cortex of CUS group ( versus the control rats:P<0. 01 ) , and chronic fluoxetine treatment reversed the CUS-induced decrease in GLT-1 levels ( P <0. 01 ) . Conclusions Chronic unpredictable stress ( CUS ) could down-regulate the GLT-1 protein levels in the prefrontal cortex, which is reversed by fluoxe-tine. These results further support the notion that en-hanced expression of the GLT-1 protein could be mo-lecular mechanism of fluoxetine antidepressant effect.

Expression of Wisp-1, β-catenin, TGF-β1 and Smad4 in gold hamster induced intrahepatic cholangiocarcinoma and their significance / 临床与实验病理学杂志

Purpose To establish a golden hamster model of intrahepatic cholangiocarcinoma (ICC) using BOP [N-nitrosobis (2-oxo-propyl) amine] and to explore the protein expression of Wisp-1,β-catenin, TGF-β1 and Smad4 in ICC and their relationship with the tumorigenesis. Methods 57 female golden hamsters aged 8 to 9 weeks (39 in experimental group, 18 in control group), the experi-mental animals were subjected to subcutaneous injection of BOP, the control group was injected with saline. The liver was removed and paraffin sections were prepared for histopathological observation. The protein expression of Wisp-1,β-catenin, TGF-β1 and Smad4 was detected by immunohistochemistry (IHC) in these blocks. Results Most of the animals in the experimental group (29/39) developed ICC, part of the animal (8/39) developed bile duct dysplasia, 1 developed focal bile duct hyperplasia, and 1 was not found bile duct hyperplasia. The positive expression rates of four protein markers in ICC, bile duct dysplasia and normal intrahepatic bile duct tissues were Wisp-1,79. 3%, 87. 5% and 5. 0%,β-catenin, 96. 6%, 100. 0% and 15. 0%, Smad4, 96. 6%, 100. 0% and 25. 0%, TGF-β1, 62. 1%, 12. 5% and 5. 0%, respectively. The positive expression rates of Wisp-1, beta-catenin and Smad4 protein in both the ICC and the tissue of bile duct dysplasia were higher than that of normal intrahepatic bile duct tissue ( P<0. 001 ) , The positive ex-pression of TGF-β1 in the ICC tissue was higher than that of normal intrahepatic bile duct tissue and bile duct dysplasia (P<0. 001). Conclusions The study showed that BOP can induce a golden hamster model of ICC and provides a reliable animal model for the study of ICC. The high expression of Wisp-1,β-catenin, TGF-β1 and Smad4 in BOP-induced intrahepatic cholangiocarcinoma is closely re-lated to occurrence, development and infiltration of ICC.

Expressions of MMP-2 and VEGF in laryngeal carcinoma and their clinicopathology meanings / 重庆医学

Objective To study the expressions of matrix metalloproteinase-2(MMP-2)and vascular endothelial growth factor (VEGF)in laryngeal carcinoma and their clinicopathological significance.Methods The immuno-histochemical technique was used to detect the expression staining results of MMP-2 and VEGF in 54 cases of laryngeal carcinoma,23 cases of polyps of vocal cord and 1 5 cases of normal laryngeal mucous tissues verified by clinicopathology under the automatic image analyzer.Results The ex-pression rates of MMP-2 and VEGF were 72.22%(39/54)and 70.37%(38/54)in the laryngeal carcinoma tissue,which were sig-nificantly higher than 47.83% and 39.13% in polyps of vocal cord and 40.00% and 46.67% in the normal laryngeal tissues respec-tively,the differences between them had statistical significance(P<0.05).The expressions of MMP-2 and VEGF had certain corre-lation with the clinical stage,lymph node metastasis and.infiltration depth of laryngeal carcinoma.Conclusion MMP-2 and VEGF may jointly participate in the process of infiltration and metastasis in laryngeal and have certain relation with the tumorous invasive growth.

Central odontogenic fibroma: Report of case with immunohistochemical study.

Objectives: To report a case of central odontogenic fibroma (COF) with immunohistochemical study. Clinical Presentation: We describe a case of epithelium‑rich type of COF in the posterior region of the mandible of a 39‑year‑old woman. Immunohistochemical examination showed the odontogenic epithelium to be positive for high‑molecular‑weight cytokeratins, vimentin and CD99, and negative for CAM5.2. The stroma contained some myofibroblasts and many fibroblast‑like cells positive for CD99. Conclusion: Our immunohistochemical findings, and especially the positive expression of vimentin from the epithelial cells of COF suggests that these cells are primordial. Last but not least, the presence of a relative small number of myofibroblasts in the stroma justifies the non‑aggressive behavior of the neoplasm and supports that a part of stromal collagen of COF is produced by these cells.

Human skin as revealed by optical and electron microscopy / 全日本鍼灸学会雑誌

[Objective]The skin is the biggest organ in the body. The human epidermis functions as a defence against various antigens in addition to physical and bio-chemical protection. The dermis consists of dense connective tissue which contains the circulatory system and sensory nerve endings. In this paper, regional differences in the structures of human skin are described.<BR>[Materials and Methods]The skin of different regions in the human body was examined by optical and electron microscopy and by utilizing various morphological techniques. <BR>[Results and Discussion]Epidermis:The cornified layer in the finger pulp and heel, which receives strong mechanical stimuli, is considerably thicker than other regions. The germinal layer consisting of spinous and basal layers becomes thinner with aging. Langerhans cells that produce antigens are scattered in the germinal layer. Furthermore, Merkel cells situated at the basal layer are found in the finger pulp, bottom of the foot and the hair disks of limbs. These cells are involved in the sense of touch or pressure. Dermis:The dermis is divided into the papillary and reticular layers, which consist of loose and dense connective tissue, respectively. In the papillary layer, fibrocytes and mast cells are distributed. Large-sized dermal papillae are found the in finger pulp and bottom of the foot, but there are also a few small papillae in other regions. In large papillae, loops of blood capillaries and Meissner's tactile corpuscles were observed. In addition, large-sized lymphatic capillaries are present in the papillary layer.A dense network of free endings, which are situated beneath the epidermis and are responsible for thermal nociception, are abundant in the face, palm, forearm and sacrum. Corpuscles of Vater-Pacini situated in the deep dermis or subcutaneous tissue are found in the finger pulp, and bottom of the foot. <BR>[Conclusion]In conclusion, it is likely that acupuncture and moxibustion may directly or indirectly stimulate Langerhans cells, Merkel cells, fibrocytes, mast cells or various nerve endings.

Role of pro-angiogenic marker galectin-3 in follicular neoplasms of thyroid.

The role of pro-angiogenic marker galectin-3 (GAL-3) was examined in differential diagnosis of follicular neoplasms of thyroid into histological subsets of follicular adenoma (FA), follicular carcinoma (FC) and follicular variant of papillary thyroid carcinoma (FVPTC). The study included 22 cases from January 2006 to June 2011 comprising of FA (n = 12), FC (n = 3) and FVPTC (n = 7). Immunohistochemical evaluation of GAL-3 was performed on representative histologic sections from the resected thyroid specimens. The proportion of stained cells and intensity of staining in tumor blood vessels were evaluated. GAL-3 expression showed that angiogenesis was prominent in malignancy (FC and FVPTC) and negative in non-neoplastic thyroid parenchyma and benign condition (FA). GAL-3 expression was found to differentiate benign from malignant follicular neoplasms. Focal and diffuse positivity for GAL-3 was found to be associated with FC and FVPTC respectively, thus GAL-3 can be used as a immunohistochemical marker in the differential diagnosis of follicular neoplasms of thyroid based on the type of expression. Limitation of this study was relatively less number of cases studied; however, this data need to be corroborated in larger cohort.

Tumor neuroectodermal melanótico de la infancia en los maxilares: reporte de dos casos / Melanotic neuroectodermal tumor of infancy on the jaws report of two cases

El Tumor Neuroectodermal Melanótico de la Infancia (TNMI) es un tumor raro que se presenta en infantes antes de un año de edad. Es un tumor de crecimiento rápido, que afecta el maxilar. En la mayoría de los casos es benigno. Reportamos dos casos atendidos en el Instituto Nacional de Enfermedades Neoplásicas. Los pacientes fueron 2 Infantes, de 4 a 7 meses de edad, en el maxilar inferior y superior respectivamente. En ambos casos se realizó resección quirúrgica del Tumor. El diagnóstico se corrobora con Inmunohistoquímica marcadores S-100 y Sinaptofísina y Panqueratina. La evolución fue favorable y ambos pacientes viven.

Melanotic Neuroectodermal Tumor of Infancy (MNTI) is a rare neoplasm occurring in infants before one year of age. It is a neoplasm of fast growth that affects maxilla. In most of the cases it is benign. We reported two cases attended at the Instituto Nacional de Enfermedades Neoplásicas. The patients were two infants, of 4 and 7 months old, in the mandible and maxilla respectively. In both cases the treatment was surgical excision. The diagnosis is corroborated with Inmunohistochemestry markers, S-100, Sinaptofisin and Pankeratin. The evolution was favorable and both patients are alive.

Comparision and application of detective methods for Her-2 gene in invasive breast carcinoma tissue / 肿瘤研究与临床

Objective Through comparision of Her-2 gene in invasive breast carcinoma tissues assayed by IHC, CISH and FISH respectively, to explore an optimal detective methods. MethodsIHC, CISH and FISH were used in detection for Her-2 gene of 100 cases, respectively.ResultsCISH identified no Her-2 gene amplification in IHC score negative rumors (6/6) and gene amplication in 7.1%(1/14) of IHC score (+) and in 32 % of IHC score (++) tumors(l6/50) and in 80 % of IHC score (+++) (24/80), Concordant rate between CISH and FISH was 100 %(20/20). ConclusionThere are advantage and disadvantage among IHC,CISH and FISH. CISH is an accurate and dependable methods and would be easy to apply for detection of Her-2 gene.

Comparasion of two different detection methods for HER-2 protein expression and gene amplification in breast cancer tissue / 中华内分泌外科杂志

Objective To compare HER-2 state in breast cancer tissue deteced by fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) and analyze their correlation. Methods HER-2/neu protein expression and gene amplification were detected by FISH and IHC in 56 newly-diagnosed cases of female breast cancer from July 2008 to July 2009. Results Of the 56 patients, HER-2 protein expression (-), (+), (++), (+++) was 9 cases (16.1%), 29 cases (51.8%), 11 cases (19.6%) and 7cases (12.5%) respectively; 26 cases (46.4%) had HER-2 gene amplification while 30 cases (53.6%) didnt have. Type of HER-2 gene amplification was mainly HER-2(++) and HER-2(+++), and according gene amplification rate was 72 7% and 100%. HER-2 (+) gene amplification rate was 37.9 %(11cases) and no gene amplification was found in HER-2(-) tissue. The HER-2 positive rate using two methods had significant difference(χ2=19.778,P<0.01). HER-2(-) and HER-2(+++) had good consistency with the FISH results(Kappa=0.969),but HER-2(+) and HER-2(+ +) were poorly consistent with the FISH results(Kappa=0.271). Conclusions IHC is the preliminary screening method for detection of HER-2 expression. HER-2(-) and HER-2(+++) have good consistency with the gene amplification, and can guide clinical treatment. Some patients with HER-2(+) and HER-2(++) have HER-2 gene amplification. FISH is needed for targeted therapy.