ABSTRACT
Objective To study the expression of HDAC2 and the correlations with pathological parameters in human tongue squamous cell carcinoma, and to analyze the correlation between them and patients' clinical indicators and survival status.Methods A total of 96 cases of humantongue squamous cell carcinoma (SCC), 24 dysplasia (ED), 13 normal mucosa (NOM) were involved in this study. The expression of HDAC2 was evaluated by ICH. Kaplan-Meier was used to research cumulative surviving of SCC patients.Result Among 96 cases of SCC samples, 86 cases showed positive expression (89.6％), The differences between the overexpression of HDAC2 and the pathological grading, lymph node metastasis, the patient's age and gender were insignificant (all P＞0.05). However, the differences of TNM presented statistically significant (χ2 = 18.600, P＜0.001). 14 out of 24 ED (58.3％) were positive expression of HDAC2, there were 7 cases (53.8％) of positive expression samples in NOM. The expression of HDAC2 in SCCs was significantly higher than that in ED and NOM (χ2= 118.407, P＜0.00). There was no significant difference of cumulative surviving between the positive and negative SCC patients (P = 0.184). However cumulative surviving of patients with lymph node metastasis was significantly shorter than those with no metastasis (P＜0.000).Conclusions The expression of HDAC2 in SCC was significantly higher than that in ED and NOM, the TNM stage of patients presented the significant difference of expression of HDAC2, patients with lymph node metastasis were significantly shorter than those with no metastasis, and demonstration can be used as a reliable reference molecule for the evaluating prognosis of SCC.
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Objective To study the roles of hepatocyte cytochrome P450 2E1 in model of nonalcoholic steatosis in rats Methods A total of 40 Wistar rats were randomly divided into two groups: control group (C) and high fat diet induced fatty liver group (H) The expression of hepatocyte cytochrome P450 2E1 antigen in rat model of nonalcoholic steatosis was detected by immunohistochemical method and Western blotting Malondialdehyde (MDA) contents were also determined Results MDA contents and the expression of hepatocyte cytochrome P450 2E1 antigen in rat model of nonalcoholic steatosis induced by high fat diet were higher than those in the normal controls ( P
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Objective To study the expression of the capsaicin receptor,vanilloid receptor subtype 1(VR1) immunoreactivity,their morphology and distribution in afferent fibers of rat esophagus. Methods Laser scanning confocal microscope combined with immunohistochemical double labeling methods were used. Results VRl-like immunoreactivity was observed on nerve fibers and terminalis within mucosa,submucosa,muscle layer and surrounding blood vessel throughout esophagus.Their profiles were fine fibers with some spiny,possessing varicose-like swellings along their lengths.About(92.3?3.7)% VR1 positive fibers co-localized with CGRP immunoreactivity,which representing large majority of afferent fibers in the esophagus was extrinsic in origin with cell bodies located in dorsal root ganglia.In dorsal root ganglion,VR1 was expressed in small-and middle-sized cell bodies.About(41.5?4.5)% VRl-immunoreactive neurons co-stained with CGRP and(67.9?3.2)% CGRP positive neurons co-localized with VR1.In nodose ganglion,the expression of VR1 was similar with dorsal root ganglion,but CGRP immunoreactive neurons very few.Only(4.7?1.4)% VR1 positive neurons co-stained with CGRP.Conclusion These results suggest that VR1 is expressed in afferent fibers in the wall of the rat esophagus which is in origin in dorsal root ganglion.
ABSTRACT
The objective of this study was to characterise the pattern of p53 mutations in bladder tumor. In this study, 25 bladder transitional cell carcinomas were analyzed by immunohisochemistry (IHC) for p53 nuclear overexpression, and the results were compared with those of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis in exon 5-8 of the p53 gene and DNA sequencing analysis. 15 out of 20 cases (75%) showed p53 nuclear immunoreactivities on IHC. On PCR-SSCP analysis, 10 out of 25 cases (40%) had abnormal shifts in mobility. 62% of the mutations were in exon 8. Direct DNA sequencing analysis were performed in these 10 cases to confirm the presence of mutated p53 genes and to determine the type of mutations. Sixteen point mutations were detected in 10 cases. Two specimens had double mutations and another two had triple mutations. G:C-->A:T transitions were the most frequent patterns (62.5%). One mutation was a premature stop codon and two were silent mutations. Three out of 10 had a point mutation at codon 285 (GAG/Glu-->AAG/Lys) and two had at codon 280 (AGA/Arg-->AAA/Lys). One of 16 mutations was transition at hot spot codon 273 with CpG site. These results suggest that altered expressions and point mutations of p53 occured in all grade of bladder cancer, but are more associated with high grade bladder tumors. To elucidate the carcinogenesis of bladder cancer, further studies should be carried out.