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Objective To observe the performances of autophagy and inlfammatory cytokines in wounded mice skin at different time, and to explore the relationship between these performances and the duration of wound in the mice. Methods RT-PCR and West-blot methods were used to detect the genetic expression and protein expression of Beclin-1, LC-3, IL-1α, IL-1β, and MCP-1 in wounded skin of the mice. Results After the mice’s skin was wounded, the level of IL-1a and IL-1β genetic and protein expression increased early, the peak was found after 12 hours, and then decreased; the expression of MCP-1 began to increase after 6 hours and it reached peak after 48 hours, then decreased; while the genetic and protein expression of LC3 and beclin-1 began to increase after 6 hours, and the peak was seen after 24hours, then decreased too. Conclusion After the mice’s skin was wounded, the starting and the peak of the expression of autophagy came later than those of inlfammatory cytokines, it is found the level of the expression of inlfammatory cytokines was showing a downward trend when the expression of autophagy reached the peak. This performance may be caused by increasing inlfammatory cytokines in the tissue of the incised wound, which activated the autophagy, and when the level of autophagy reached a certain degree, it could suppress the excessive inlfammatory reaction. So the autophagy and the inlfammatory cytokines interact regularly after the mice’s skin was wounded, and such interaction offers us a reference to infer the injury time after injury.
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The ethanolic extract of S. robusta resin (10 and 30 % w/w applied locally in excised and incised wounds) produced a dose-dependent acceleration in wound contraction and increased hydroxyproline content and tensile strength of wounds in rats. The results demonstrate wound healing activity of ethanolic extract of S. robusta resin.
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Objective:To investigate whether the expression of protein VEGF and TGF-?1 can be used as an indextoestimate injuryage in the field of forensic medicine by approaching the temporal relation of VEGF and TGF-?1 expression with injury in the healing process of the incised wounds on rat skin.Methods:Experimental rat models were established by incisions made on the rat skin.the rat models were divided randomly into three groups:the experimental group,the normal group,and the postmortem injured group.The expression of VEGF and TGF-?1 were observed with Immunohistochemical techniques in their process of cicatrization.Results:In the experimental group,the expression of VEGF and TGF-?1 were higher than those in the normal control and postmortem injury group(P
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Objective:To explore the expression of MIP-1? during healing of skin incised wound and the applicability of time-dependent expressions of MIP-1? to determination of wound age. Methods:An incised wound was made in the dorsal skin of mouse. Immunohistochemical and image-analysis techniques were employed in vital skin wounds 1h~14d after incision and postmortem incision 0.5~6h after death, and the non-incised mouse skin was used as contro1.Results:In the vital skin incisions,expression of MIP-1? began at 6h after incision,which increased subsequently,and peaked at 3d.The quantity of MIP-1?expression decreased and minimized in the specimens aged 14 days. In the non-wounded groups and postmortem incision groups MIP-1? was only detected as weak expression in the epidermic cells,sweat gland cells and endothelial cells. Conclusion:The time-dependent expression of MIP-1?during healing of skin incised wound may be used as a useful reference marker.
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The content of fibronectin in skin incised wound of adult abdomen was rneasured with double antibody sandwich EI,lSA is report.The result indicated that it is possible to measure fibronectin in extracts of skin tissue. The mean extractable fibronectin level per gram adult abdominal skin was 7. 5920?1. 7364ug (M ? SD ). The content of fibronectin in incised wound at different groups in lccal area intervals after injury increased gradually,acconlpy1ng along with the tirne prolonged. There existed a linear correlation between the tlme elapsed after 1n jury and the fibronectin content in wounds. But,only O. 5hr or longer than 0. 5hr after injury the difference between local skin fibronectin and instantenous one is significant (P
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Enzyme-histochemical investigations on experimental incised injury of thirty rabbits fortiming of wound were reported.In peripheral areas of wounds,strong reaction of six kinds ofdehydratase(ACP.AKP,ATP.ANAE.?—GA,?—Gr).were demonstrated 1～2 hrs followingincision while no reaction of four kinds of dehydrogenase(LDH.SDH.NADH.?—GPDH)werefound.The activity of dehydratase are explained by the enhanced metabolism of fibrocytes and theaggregation of acute inflammatory cells.The author suggests that the positive enzyme histochemicalreactions indicate the vital reaction in the first two hours,and PTAH staining is helpful for thediagnosis of micro—exudation of fibrin in the early stage of injury
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Objective To explore the time-related expressions of TGF-?1mRNA during the healing process of rat skin wounds. Method Using the method of the reverse transcriptase polymerase chain reac-tion (RT-PCR) on intra-vital rat skin incised wounds (0.5h, 1h, 3h, 6h, 48h, 72h, 96h, 168h after incision) and postmortem rat skin incised wounds (0.5h, 1h, 3h after incision) to detect the dynamics of expression Ievel of TGF-?1mRNA. The strength of the expression of TGF-?1mRNA in scanned image was using ID-Advanced software. Results The results of RT-PCR showed that TGF-?1mRNA Ievel increased at 0.5h after incision and elevated significantly after 3h. The peak of TGF-?1mRNA occurred at 48h. There was no obvious expression of TGF-?1mRNA in postmortem incised wounds. Conclusion The characteristics of the TGF-?1mRNA expression were potentially indicative for the wound aging. RT-PCR was a sensitive method for detection of the expression of cytokines in genic level.