Helicobacter pylori infection factors and immunological typing in community health examination population / 公共卫生与预防医学

Objective To investigate and analyze the risk factors of Helicobacter pylori (Hp) infection and the immunological classification of Hp-infected people in community health examination population. Methods Among of the 2 475 residents in a community who underwent health examination in Guangyuan First People's Hospital were selected between July 2022 and April 2023. Western blotting was used to detect and type the Hp antibodies. The risk factors of Hp infection in the community residents were analyzed by binary logistic regression analysis, and the results of immunological typing were compared among patients with different upper digestive tract diseases and chronic diseases. Results The Hp infection rate among of the 2 475 residents who underwent physical examination was 35.64% (882/2475), and the positive rates of type I Hp antibody and type II Hp antibody were 23.72% (587/2 475) and 11.92% (295/2 475) , respectively. There were significant differences in Hp infection rates in gender, age, smoking history, and whether or not people had diabetes (P<0.05). Multivariate logistic regression analysis showed that male, age ≥60 years old, drinking history and diabetes were risk factors of Hp infection in the community health examination population (P<0.05). The positive rate of type I Hp antibody in men and patients with coronary heart disease was higher than that in women and patients without coronary heart disease (P<0.05). Conclusion The infection rate of Hp among health examination population in this community is low, mainly type I. Hp infection is affected by gender, age, drinking history and diabetes. There are more males in the population with type I Hp antibody positivity, and the risk may be higher in patients with coronary heart disease. Clinically, it is necessary to take susceptible population as the focus of prevention and treatment, and take targeted monitoring and diagnosis and treatment measures.

Triatomine vectors of Trypanosoma cruzi in an endemic area for Chagas disease in Northeast Brazil

ABSTRACT Background: We assessed the distribution of triatomines in an endemic area for Chagas disease. Methods: This retrospective study used secondary data extracted from the Official System of the National Chagas Disease Control Program (Sistema Oficial do Programa Nacional de Controle da Doença de Chagas - SisPCDCh). Results: A total of 7,257 (725.7 ± 221.7 per year) specimens were collected from 2013 to 2022. Most of them (6,792; 93.6%) were collected in the intradomicile and 465 (6.4%) in the peridomicile. A total of 513 (7.1%) triatomines tested positive for the presence of trypomastigote forms, similar to Trypanosoma cruzi. Conclusions: The spatial analysis revealed a heterogeneous distribution of triatomines across different municipalities.

The impact of bariatric and metabolic surgery on the morbidity and mortality of patients infected during the COVID-19 pandemic: a retrospective cohort study

ABSTRACT BACKGROUND: Since the impact of the coronavirus disease 2019 (COVID-19) pandemic in March 2020, several studies have shown a strong relationship between obesity and severe cases of COVID-19. It is imperative to assess whether bariatric surgery exerts a protective effect in such cases. OBJECTIVE: This study aimed to assess the impact of bariatric surgery on the morbidity and mortality in obese patients during the COVID-19 pandemic. A comprehensive search was performed using the PubMed and Cochrane Library databases. DESIGN AND SETTING: Retrospective cohort studies conducted in the Faculdade de Medicina da Universidade Cidade de São Paulo, São Paulo (SP), Brazil. METHODS: The search comprised the following descriptors: "bariatric, surgery, COVID-19". Current retrospective cohort studies that examined the influence of bariatric surgery on the morbidity and mortality of obese patients during the COVID-19 pandemic were considered eligible. RESULTS: After removing duplicates, 184 studies were obtained from the databases. Of these, 181 were excluded from the analysis as they did not meet the eligibility criteria. Patients undergoing postoperative follow-up of bariatric surgery had a similar probability of SARS-CoV-2 infection compared to the general population, and persistent comorbidities were associated with an increased risk and severity of infection. CONCLUSION: Bariatric surgery has a protective effect against severe COVID-19 in the obese population, bringing the prevalence of severe disease cases to levels equivalent to those of the nonobese general population, with a positive impact on morbidity and mortality.

COVID-19 Infection Rate following COVID-19 Vaccination among Healthcare Professionals at a Tertiary Care Public Hospital in City of Mumbai

Background : India launched COVID-19 mass Vaccination campaign after regulatory approval of Covishield & Covaxin vaccines. However, inspite of Vaccination, cases of COVID-19 infection are being reported. Hence, this study is aimed to assess the COVID-19 infection and/or re-infection rate, including breakthrough infections, following vaccination among Health Care Workers at a Tertiary Care Dedicated COVID Hospital. This questionnaire-based survey was initiated following Institutional Ethics Committee approval. We also looked at reasons for Vaccine hesitancy and occurrence of adverse reactions following vaccination, their management and duration amongst the Healthcare Workers. Results : Of 564 Healthcare Workers (HCWs) who consented to participate, only 503 filled in the questionnaire completely. Majority of the HCWs received Covishield vaccine (78.56%). The infection rate postvaccination was 8.28% (with a median of 22 days and IQR of 8-43 days). This infection rate was significantly higher in those who were not vaccinated as compared to the Vaccinated HCWs (OR = 0.10, 95 CI% = 0.05�22, p <0.0001). Breakthrough infection rate was 2.42. Although 58.39% of the participants suffered adverse reactions after vaccination, like myalgia, Fever, Headache, these were mild in nature lasting for an average of 3-4 days. The vaccine hesitancy rate at our hospital HCWs was 6.36%, the main issue being the concerns regarding safety and effectiveness of the vaccines against the COVID-19 infection. Discussion/Conclusion : The infection and Breakthrough infection rates in our study were low and severity of COVID infection post vaccination was mild, not requiring hospitalisation

Infestación de Musca domestica L. (Díptera, Muscidae) en faenadoras avícolas / Infestation of Musca domestica L. (Diptera, Muscidae) in poultry slaughterhouses

Musca domestica (Diptera, Muscidae) es la mosca más común en todo el mundo. Más de 100 patógenos pueden causar enfermedades en humanos y animales por este tipo de insectos. Estos patógenos incluyen: diarrea infantil, ántrax, cólera, oftalmía, disentería bacilar, fiebre tifoidea y tuberculosis. Además, las moscas domésticas transmiten muchos de los huevos de helmintos como Enterobius vermicularis, Strongyloides stercoralis, Trichuris trichiura, Toxocara canis, y especies de Dipylidium, Diphyllobothriam, Hymenolepis, Taenia y Trichomonas. También puede transmitir quistes de protozoos y trofozoítos como E. histolytica y Giardia lamblia. Se determinó el índice de infestación por Musca domestica L. (Díptera, Muscidae) en una faenadora avícola ubicada en el Perú. Para ello se evaluó no sólo el tratamiento aplicado: protocolo de cola entomológica (T1), monitoreo de insfectación de mosca (T2) o comincación de lámparas LED UV más tabla de pegamento adhesivo (T3), sino también las diferentes zonas de faenadora: zonas sucias de descarga y estabulación (ZDE), preparación (ZP), zonas adicionales de sacrificio sanitario (ZSS), preparación de residuos orgánicos (ZPRO), conservación de los residuos orgánicos (ZCRO) y de conservación de sangre (ZCS). Los resultados demoestraron que, independientemente de la zona de operación, el tratamiento más efectivo para determinar la infestación por la mosca doméstica fue el combinado de lámpara LED junto con tablas de pegamento (T3), seguido del tratamiento de protocolo de cola entomológica (T1) y el tratamiento por Monitoreo de infestación de moscas (T2) independientemente de los días de recolección. Es importante seguir las diferentes normativas a fin de controlar y eliminar la presencia de moscas (y de otros insectos voladores) para evitar la contaminación y, por ende, enfermedades(AU)

Musca domestica (Diptera, Muscidae) is the most common fly in the world. More than 100 pathogens can cause diseases in humans and animals by these types of insects. These pathogens include: infant diarrhea, anthrax, cholera, ophthalmia, bacillary dysentery, typhoid fever, and tuberculosis. In addition, houseflies transmit many of the eggs of helminths such as Enterobius vermicularis, Strongyloides stercoralis, Trichuris trichiura, Toxocara canis, and Dipylidium, Diphyllobothriam, Hymenolepis, Taenia, and Trichomonas species. It can also transmit cysts of protozoa and trophozoites such as E. histolytica and Giardia lamblia. The infestation index by Musca domestica L. (Diptera, Muscidae) was determined in a poultry slaughterhouse located in Peru. For this, not only the applied treatment was evaluated: entomological glue protocol (T1), fly infestation monitoring (T2) or combination of UV LED lamps plus adhesive glue table (T3), but also the different slaughter areas: unloading and holding areas (ZDE), preparation (ZP), additional stamping-out areas (ZSS), preparation of organic waste (ZPRO), conservation of organic waste (ZCRO) and blood conservation (ZCS). The results showed that, regardless of the area of operation, the most effective treatment to determine housefly infestation was the LED lamp combined with glue boards (T3), followed by the entomological glue protocol treatment (T1 ) and the treatment by Monitoring of fly infestation (T2) regardless of the days of collection. It is important to follow the different regulations in order to control and eliminate the presence of flies (and other flying insects) to avoid contamination and, therefore, diseases(AU)

Effect of Different Combinations of Arbuscular Mycorrhiza on Paris polyphylla var. yunnanensis and Chemical Components / 中国实验方剂学杂志

Objective:To study the effects of different arbuscular mycorrhizal（AM） fungi combinations on the growth and quality of<italic> Paris polyphylla</italic> var. <italic>yunnanensis</italic> seedlings，in order to provide reference for the cultivation of high quality <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>. Method:Through the room temperature pot inoculation test method，nine groups of different AM fungi combinations were inoculated into sterilized soil，and the control group was not inoculated. The effects of different AM fungi combinations on root infection rate，photosynthetic parameters，physiological indexes and chemical components of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> seedlings were observed. Result:The spore density in rhizosphere soil，the infection rate and intensity of AM fungi in roots，and the activities of succinate dehydrogenase and alkaline phosphatase in roots of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> were significantly increased by inoculating the combination of exogenous AM fungi. Compared with the control group，the contents of chlorophyll，malondialdehyde，soluble sugar，soluble protein content and the activities of antioxidant enzymes in leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> were increased in different AM fungi treatment groups. The content of total saponin increased from T1 to T2，decreased rapidly from T2 to T4，and increased at T5. Conclusion:Different mixed AM fungus can form a good symbiotic relationship with the roots of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>，improve the mycorrhizal vitality，enhance the resistance of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> to adverse environment，promote the growth and development of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> and improve the quality of rhizome. According to the comprehensive inoculation effect，the combination of S5，S8 and S9 AM fungus has the best effect，which provides reference value for the application of mycorrhizal biotechnology of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>.

Influences of Natural Fibrous Root Decay on Growth and Active Component Content of Paris polyphylla var. yunnanensis / 中国实验方剂学杂志

Objective:To explore the effect of natural decay of exogenously added fibrous roots on the growth and development of <italic>Paris polyphylla</italic> var. <italic>yunnanensis </italic>and its medicinal quality. Method:The effects of natural decay of fibrous roots at different amounts on mycorrhizal infection rate， physiological and biochemical indexes， and saponin contents of <italic>P. polyphylla</italic> var. <italic>yunnanensis </italic>were studied in pot culture experiments at room temperature. Result:The results showed that the infection rate of arbuscular mycorrhizal （AM） fungi in the root of <italic>P. polyphylla</italic> var. <italic>yunnanensis </italic>was not significantly affected by different fibrous root treatments， but there were significant differences in infection intensity. The photosynthetic pigment content in the leaves declined significantly with the increase in fibrous root amount， and the total chlorophyll was decreased by 78.7% at most. The contents of soluble protein， soluble sugar and malondialdehyde in the leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis </italic>showed an overall upward trend. The activities of the three protective enzymes varied. The peroxidase and malondialdehyde were reduced by 181.6% and 200.0% at most. In the root system of <italic>P. polyphylla </italic>var. <italic>yunnanensis</italic>， the contents of the above-mentioned six components decreased to varying degrees， with the largest reductions of peroxidase and malondialdehyde reaching 44.6% and 69.7%. Different fibrous root treatments resulted in a decrease in active component content of <italic>P. polyphylla </italic>var. <italic>yunnanensis</italic>. The total content of the four saponins was decreased by 58.9% at most， and the total saponin content by 46.9%. Conclusion:The natural decay of fibrous roots affects the soil microbial environment of root system， reduces the photosynthetic pigment content in leaves， and destroys the stability of cells， thus interfering with the growth and development of <italic>P. polyphylla </italic>var. <italic>yunnanensis</italic>， reducing its medicinal components， and causing continuous cropping obstacles.

HCV infection in 219 heroin addicts with pulmonary tuberculosis in Xi’an / 西安交通大学学报(医学版)

【Objective】 To explore the incidence rate and characteristics of hepatitis-C virus (HCV) infection in heroin addicts with pulmonary tuberculosis (TB) in Xi’an so as to provide a basis for pulmonary TB and HCV prevention and control measures. 【Methods】 We carried out behavior investigation and serological detection of 219 heroin addicts with pulmonary TB infection in Xi’an Eighth Hospital. We analyzed HCV infection risk factors by Chi-square test and multi-factor Logistic regression method. 【Results】 The prevalence of HCV infection in heroin addicts with pulmonary TB was 71.2% and 92.9% in injected drug users (IDUs). The HCV infection rate was higher than the infection rate of HBV (10.9%) and HIV (5.9%) in heroin addicts with pulmonary TB. The major infection risk factors were intravenous injection (OR=18.632, 95% CI: 8.146-41.284) and longer duration of drug use (OR=1.428, 95% CI: 1.086-1.876). 【Conclusion】 The study found high HCV infection rate in heroin addicts with pulmonary TB and that the independent risk factors are the drug injection and duration of drug use. In order to prevent further spread of HCV in heroin addicts with pulmonary TB, we should establish and perfect the management system of HCV infection.

Investigation of the Demodex infection in eyes of patients with recurrent chalazion / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the Demodex infection in patients with recurrent chalazion and the possible related factors for Demodex infection.<p>METHODS: Patients diagnosed with "chalazion" in our ocular surface and cornea department from January 2019 to May 2019 were collected. 32 eyes in group A were patients with recurrent chalazion, 30 eyes in group B were patients with primary chalazion, and 35 eyes in group C were patients without eye disease. The positive infection rate of the roots of Demodex lashes was observed by biological optical microscopy. The infection of Demodex in the roots of the eyelashes and the opening of the meibomian glands was observed by vivo laser scanning confocal microscopy.<p>RESULTS: Observations by biological optical microscope: the detection rate of Demodex in the eyelashes of group A was 78%, which was significantly higher than that of group B(57%)and group C(34%). Observations by confocal microscopy: the detection rate of Demodex in the eyelashes of group A was 88%, which was significantly higher than that of group B(67%)and group C(37%). The detection rate of Demodex in the meibomian gland opening of group A was 69%, which was significantly higher than that of group B(23%)and group C(14%).<p>CONCLUSION: The rate of Demodex infection in patients with recurrent chalazion is obviously higher. Demodex infection may be one of the pathogenic reasons for recurrent chalazion.

Effect of Field Inoculation with Arbuscular Mycorrhizal Fungi on Rhizosphere Soil Structure of Paris polyphylla var. yunnanensis / 中国实验方剂学杂志

Objective:To investigate the effects of arbuscular mycorrhiza（AM） fungi inoculation on the rhizosphere soil of Paris polyphylla var. yunnanensis under field conditions，so as to provide a reference for the standardized cultivation and development of high-quality varieties of P. polyphylla var. yunnanensis. Method:The effects of inoculation with mycorrhizal fungi on the rhizosphere soil structure of P. polyphylla var. yunnanensis were observed through a combination of small-scale field inoculation test and laboratory analysis. Soil indexes were determined by soil agrochemical methods. Result:The treatment groups inoculated with exogenous AM fungi showed a regulatory effect on the infection rate and intensity of AM fungi infection in the root system of P. polyphylla var. yunnanensis. After treatment with AM fungi，the soil pH was basically not affected，and the contents of organic matter，available nitrogen，available phosphorus，and available potassium increased. Moreover，the number of fungi decreased，the number of bacteria and actinomycetes increased，and soil enzyme activities increased. The results of correlation analysis showed that there was a significant positive correlation among the soil physical and chemical indexes，especially the bacterial number and the three types of phosphatases showed extremely significant correlation （r=0.849，0.800，0.804，P<0.01）. Conclusion:The application of the two mixed fungicides could increase the number of microorganisms and enzyme activities in the rhizosphere of P. polyphylla var. yunnanensis， and there was a certain synergy effect among the soil factors. Among the three field trials，the effects in Anshun，Guizhou and Wanzhou，Chongqing were more ideal，which provided a theoretical and practical basis for large-scale promotion of P. polyphylla var. yunnanensis in the field.

Meta-analysis of bone morphogenetic protein versus autologous bone grafting for limb long bone nonunion / 中国组织工程研究

BACKGROUND: Bone morphogenetic proteins have strong bone induction properties and have been proved to promote bone healing in fracture, bone defect and other diseases. However, few studies are reported on the use of bone morphogenetic proteins in the treatment of bone nonunion, and the results of various studies remain controversial, which makes the role of bone morphogenetic proteins in the treatment of limb long bone nonunion unclear. OBJECTIVE: Meta-analysis was used to systematically evaluate the advantages and disadvantages of bone morphogenetic protein versus autogenous bone grafting in the treatment of limb long bone nonunion. METHODS: PubMed, Elsevier, Web of Science, Cochrane Library, CNKI and WanFang databases were searched to retrieve the randomized controlled trials and non-randomized controlled trials of bone morphogenetic proteins for limb long bone nonunion published before April 2019. Quality evaluation and data extraction of the included literatures were performed. Meta-analysis of outcome indicators was performed using RevMan 5. 1 software provided by Cochrane system. RESULTS AND CONCLUSION: Eight articles were enrolled, including 4 randomized controlled trials and 4 non-randomized controlled trials, all of which were small sample-size studies involving 30-124 cases. A total of 613 cases of nonunion were included. In the study group, patients received bone morphogenetic proteins or bone morphogenetic proteins in combination with bone grafting. In the control group, patients received autologous bone grafting. Meta-analysis results showed that there were no significant differences in postoperative healing rate, infection rate, secondary operation rate, and postoperative improvement in limb function between study and control groups (P > 0. 05). The mean healing time in the study group was significantly shorter than that in the control group [WMD=-1. 24, 95%C/(-1. 70,-0. 79), P 0. 05). These results suggest that bone morphogenetic protein can provide a viable alternative to autologous bone grafting, and it is also a safe adjuvant for autologous bone grafting, which has the potential advantage of accelerating fracture healing, but the current evidence does not support bone morphogenetic protein in combination with autologous bone grafting.

The impact of meteorological and environmental conditions on the spread of COVID-19 / 公共卫生与预防医学

Objective To understand the impact of meteorological and environmental conditions on the spread of COVID-19, and to provide a scientific basis for prediction, prevention and control of COVID-19. Methods The number of confirmed cases, population density and meteorological data of all cities in Hubei Province during COVID-19 outbreak period were collected. The relationship between COVID-19 infection rate and climatic factors were analyzed using Excel and SPSS software. Results The infection rate of COVID-19 had the greatest correlation with population density. The infection rate of COVID-19 showed a very significant power-law relationship with the number of the hot days before COVID-19 outbreak, and it also had a good correlation with the high temperature accumulation, the rank of hot days and the drought intensity. Conclusion The adverse effect of high temperature and heat wave on the human body had an impact on the spread of COVID-19. The ability of people in different areas to resist high temperature was different because of different climates. Drought may affect the spread of COVID-19 by destroying the ecological environment.

Analysis of the status and influencing factors of Helicobacter pylori infection of electrical power system workers in Shanghai / 公共卫生与预防医学

Objective To investigate Helicobacter pylori (HP) infection status and influencing factors of electrical power system employees in Shanghai. Methods A total of 29 986 employees in Shanghai electrical power system who underwent physical examination were selected as the research subjects, and received 13C-urea breath test (13C-UBT). The HP infection rate was recorded. Data including gender, age, body mass index, smoking history, drinking history, medication history, educational level, consumption of high-salt and high-fat food, presence of vomiting, and awareness of HP infection related treatment were also collected. The relationship between these factors and HP infection was analyzed. Results 13C-UBT showed that of 29,986 subjects examined, 17 580 were HP positive, and the positive rate was 58.63%. There was significant difference in HP infection rate between men and women (P 0.05). Logistic regression analysis showed that male, ignorance of HP infection related treatment, smoking and history of family HP infection were independent influencing factors for HP infection. Conclusion The HP infection rate was relatively high among employees in Shanghai electrical power system, and the main influencing factors of HP infection were male, ignorance of HP infection related treatment, smoking and history of HP family infection.

Preliminary investigation on arbuscular mycorrhiza fungi of cultivated Panax quinquefolium roots / 中国中药杂志

In this study, the infection of root arbuscular mycorrhizal fungi(arbuscular mycorrhizal fungi, AMF of Panax quinquefolium in Shandong province was investigated, and the distribution characteristics and infection regularity of AMF were found out. The AMF of P. quinquefolium roots in different habitats was examined by alkali dissociation-trypickin blue staining method to study the infection rate and infection intensity. The contents of ginsenoside(Rb_1, Re, Rg_1, Rb_2, Rd and Rh_1) in the roots of P. quinquefolium was determined by HPLC. The experimental data were SPSS 17.0 statistical software for One-way analysis of variance, cluster analysis and correlation analysis. The results showed that the AMF infection in roots of P. quinquefolium, and there were obvious structures such as hyphae, arbuscular branches and vesicles, and the AMF infection rate and infection intensity showed obvious spatial and temporal heterogeneity with the growth age and origin of P. quinquefolium. The infection rate of AMF in roots of P. quinquefolium from 1 to 3 years increased significantly with the increase of growth years(P<0.05). The infection intensity and infection rate of P. quinquefolium showed a similar change trend, the AMF infection rate and infection intensity reached the highest level in the third year. Cluster analysis showed that the infection rates of roots of P. quinquefolium in similar geographical locations could be clustered together. Correlation analysis showed that the AMF infection rate of P. quinquefolium root was significantly positively correlated with the infection intensity, and the AMF infection rate and infection intensity were significantly positively correlated with the contents of ginsenoside Rg_1, Re and Rb_1. This study explored the distribution characteristics and regularity of AMF in roots of P. quinquefolium under the protected cultivation conditions, and provided basic data for ecological cultivation of P. quinquefolium and research and development of biological bacterial fertilizer.

Research on Helicobacter pylori infection and risk factors in physical examination population in Luzhou / 实用医学杂志

Objective To investigate the infection rate and risk factors of Helicobacter pylori in healthy people in Luzhou. Methods From May 2017 to May 2018, the number of physical examinations for Helicobacter pylori infection was 18, 684 in the Affiliated Hospital of Southwestern Medical University of Ganzhou, the Affiliated Hospital of Southwestern Medical University and Cangzhou People 's Hospital and Jixian People's Hospital.C14, C13 breath test or Hp antibody positive could be considered as Helicobacter pylori infection. Eight hundred people were randomly selected to conduct a telephone survey. The survey content included general information, living habits, blood type and personal and family gastrointestinal related past medical history. Results The HP infection rate of the medical examination population in Cangzhou City was 31.6％, the male HP infection rate was32.4％ (3788/11836) , the female HP infection rate was 30.4％ (2086/6848) , and the male HP infection rate was higher than that of the female (P = 0.025). Univariate analysis showed that gender, BMI, drinking, drinking water, frequent eating, family members and the previous digestive tract diseases, and previous history of oral disease were risk factors for Helicobacter pylori infection. Results of multivariate logistic analysis showed Male, BMI, drinking, drinking water, eating out often, family members and the digestive tract disease, and a history of oral disease were risk factors for Helicobacter pylori infection. Conclusion BMI, drinking water, and eating out in the medical examination population of Quzhou City, family members, the digestive tract diseases and previous history of oral disease are risk factors for Helicobacter pylori infection.

Effects of different inoculation periods on seedling growth and steroidal saponin content of Paris polyphylla var. yunnanensis / 中草药

Objective: To study the effects of different inoculation periods on seedling growth and steroidal saponin content of Paris polyphylla var. yunnanensis, in order to lay a foundation for cultivating and domesticating high-quality Paris polyphylla var. yunnanensis. Methods: The content of steroidal saponins I, II, VI, and VII in the different vaccination periods of Paris polyphylla var. yunnanensis was determined by HPLC. Meanwhile, trypan blue, UV spectrophotometry and so on were adopted to explore colonization rate, colonization intensity, physiological and biochemical indexes, rhizome biomass of the roots of Paris polyphylla var. yunnanensis. Results: Infection rate of AM fungi was higher in different inoculation periods, activities of protective enzymes, photosynthetic pigments and soluble sugars were increased, soluble protein was not changed, content of MDA was decreased, stress resistance of the seedlings of Paris polyphylla var. yunnanensis was improved, and the growth and development were good. Quality of the cultivar one-year seedlings (recovered in August 2015, T7) was relatively low, and cultivar one-year seedlings (harvested in June or July 2015, T5 and T6) and cultivar two years seedlings (recovered in August 2015, T8) were the best. S2, S3 and S6 treatment groups had better effect in different AM fungi mixed groups. Conclusion: Seedling growth and steroidal saponin content of Paris polyphylla var. yunnanensis inoculated by different inoculation periods had certain effects.

Insect vectors of Chagas disease (Trypanosoma cruzi) in Northeastern Brazil

Abstract INTRODUCTION: Chagas disease remains a public health problem in the rural and urban areas of 19 countries in the Americas. METHODS: The aim of the present study was to investigate the Trypanosoma cruzi infection rate of triatomines collected from both intra- and peridomiciliary areas in eleven municipalities of Southeastern Ceará, Brazil, from 2009 to 2015. RESULTS: A total of 32,364 triatomine specimens, including nymphs and adults, were collected, and 31,736 (98.06%) of these were examined. More nymphs were collected than adults, and the greatest number of triatomines (n = 8,548) was collected in 2010, for which the infection rate was 1.3%, with the highest rate of infections observed for specimens from Quixere. The species collected during the study were identified as Triatoma pseudomaculata, Triatoma brasiliensis, Panstrongylus megistus, Panstrongylus lutzi, and Rhodnius nasutus, with T. pseudomaculata being the most abundant (n = 19,962). CONCLUSIONS: These results verify the presence of triatomines in both intra- and peridomiciliary areas, thereby ensuring persistence of the pathogen and consequently, the disease, as the presence of infected vectors in households is an important risk factor. According to these findings, the Chagas Disease Control Program should intensify its efforts in order to prevent the spread of the disease.

Survey on the rate of Helicobacter pylori infection in 12 932 cases of children in Kunming area / 中国医师进修杂志

Objective To investigate the prevalence and trend of Helicobacter pylori (HP) infection in children with gastrointestinal symptoms in Kunming area, and to provide basis for prevention and cure. Methods All of 12 932 cases with gastrointestinal symptoms were adopted by retrospective analysis method from January 2013 to December 2016, and HP infection was detected by 13C-urea breath test (13C-UBT). The epidemioloqical characteristics of children with HP infection in including prevalence trend and gender and age HP were statistically analyzed. Results The rates of HP from January 2013 to December 2016 were respectively 17.85%, 18.26%, 20.41% and 32.93%.The rate of HP positive was 24.08%(3 114/12 932), which was 24.82%(1 706/6 874) in boys and 23.24%(1 408/6 058) in girls. The rate of HP infection was significantly higher in boys than that in girls, and there was statistical differences (P＜0.05). The rate of HP infection showed a gradual upward trend with age. There was statistical differences in gender between age 11 and 14[(29.03% (349/1 202) vs. 22.62% (190/840)] (P＜0.05). Conclusions HP infection rate is high in the children with gastrointestinal symptoms in Kunming, and the rate of HP infection in children is related with gender .The rate of HP infection is significantly higher in boys than that in girls, and the positive rate is directly proportional.

Evaluation of hepatitis C virus infection based on big data of hospital / 中华肝脏病杂志

Objective@#To evaluate the feasibility of hepatitis C virus (HCV) infection by survey methods based on big data of hospital.@*Methods@#Inpatients data of anti-HCV was collected in 2016 and 2011. Patient’s data related to Department of Liver Diseases were excluded. The research population was divided into Surgical and Non-surgical Department. The characteristics of the two groups were analyzed and the changing trends of anti -HCV positive rate in different years was compared and analyzed.@*Results@#Patients in the surgical and non-surgical department of hospital were equally distributed across gender, urban and rural areas, and region, but the distribution of patients in surgical departments were relatively equal in all age groups. The positive rate of anti -HCV in hospitalized patients in 2016 was 0.82%, and anti -HCV positive rate was 0.58% in surgical department of 1~55 years old. Among them, anti-HCV was positive in 0.06% who underwent surgical procedure at the age of ≤25 (born after 1993 and screened for hepatitis C antibody), which was significantly lower than those in other age groups were. The anti -HCV positive rate of patients (all age groups) was lower in 2016 than that in 2011(0.75% vs. 0.97%). The anti-HCV positive rates of surgical department in both years had a decreasing trend with age.@*Conclusion@#The prevalence rate of anti-HCV, among patients population of operation departments, might reflect the prevalence rate of HCV infection among general population. Using the convenience of hospital data acquisition, we might dynamically understand the change of HCV infection.

Analysis of human papillomavirus infection status and genotype distribution in women in Huzhou, China / 中华实验和临床病毒学杂志

Objective@#To observe the infection status and the distribution of the gene subtypes of human papillomavirus (HPV) in Huzhou.@*Methods@#Cervical exfoliated cells were collected from the women who attended the physical examination and the cervical HPV examination from June 2017 to December 2017 in Huzhou Maternal and Child Health-Care hospital. A total of 2 830 women were tested for 21 HPV genotypes (including HPV-16, 18, 31, 59, 66, 53, 33, 58, 45, 56, 52, 35, 68, 51, 39, 82, 26, 73, 6, 11, 81), using real-time polymerase chain reaction (RT-PCR), investigated the infection status and subtype distribution.@*Results@#Among the 2 830 women, 269 were positive for HPV, the total infection rate was 9.51% (269/2 830). The infection rate of single gene and double gene was 86.99% (234/269) and 11.90% (32/269), respectively. The infection rate of high-risk subtypes was 9.11% among the detected women (258/2 830), accounting for 83.77% of HPV gene detections (258/308). The HPV-58, 16, 52 were the most commonly found gene subtypes among the high-risk HPV. The rate of HPV infection and high-risk subtypes of HPV infection in women was the highest in the 56-60 year old group, and the lowest in the 31-35 year old group. In different age groups, the difference in high-risk HPV infection rate was statistically significant, the total infection rate had no statistical significance.@*Conclusions@#In Huzhou area, the HPV infection rate of women found through normal physical examination was 9.51%, the high risk type was 58, 16 and 52, and the 56-60 year old group was the high incidence group. This study provides the HPV infection status and HPV gene subtype distribution characteristics in women in Huzhou, which has important guiding value for the vaccine development and application, and for prevention of HPV infection.