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Objective: Optimizing the formula and preparation of β-elemene- polybutylcyanoacrylate nanoparticles (β-ELE-PBCA-NP). Methods: With polybutylcyanoacrylate (PBCA) as the drug-loaded material, β-ELE-PBCA-NP was prepared by interfacial polycondensation. The preparation conditions were optimized by single factor design and the formula was optimized by orthogonal design with entrapment efficiency (EE) as index. EE of β-ELE-PBCA-NP was determinated by RP-HPLC. Results: Under the optimal conditions, the prepared NPs were round and regular in shape without adhesions with average particle size of 254 nm and EE of 90.17%. Conclusion: The optimized technology in this experiment is stable and feasible.
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OBJECTIVE:To optimize the formula and preparation technique of oleanolic acid loaded polybutylcyanoacrylate nanocapsules(OA-PBCA-NC)by interfacial polymerization and to study its quality.METHODS:The preparation technique of OA-PBCA-NC prepared by interfacial polymerization were optimized by single factor design,and the formula was optimized by orthogonal design with entrapment ratio as index.Its shape,particle size and the entrapment ratio were investigated as well.RESULTS:The optimized dosage ratios were as follows∶ OA vs.BCA∶60mg∶0.1ml;acetic ether vs.BCA∶2.0ml∶0.1ml;and oil vs.water:1∶1.The prepared nanocapsules were round and regular in shape without adherence.The particle size was well-distributed with mean diameter at(214?8)nm and mean entrapment ratio at(76.8?0.4)%.CONCLUSION:The preparation technique of OA-PBCA-NC established in this study is stable and feasible.
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Objective To optimize the preparation of nanoparticles encapsulating antisense oligodeoxynucleotides in a-butyleyanoacrylate carrier (ASODN in NP) and investigate their stability. Methods ASODN in NP were prepared by interfacial polymerization of butyleyanoacrylate (BCA). The formulation and technology of the prepared NP was optimized by using orthogonal design based on the single-factor experiment. The morphology of NP was examined by transmission electron microscope; The size and size distribution of NP were determined by Malvern laser granularity equipment;The encapsulation efficiency and drug loading were determined by HPLC; The ability of protecting oligodeoxynucleotides from serum was investigated on a 20% polyacrylamide-7 Murea sequencing gel (PAGE). Results The nanoparticles in the optimal conditions were of regular spherical surface and discrete. The average size was 97.1 nm,the average encapsulation efficiency and drug loading of ASODN in NP were 96.7% and 10.1% respectively; The oligonucleotides were more efficiently protected from degradation by nucleases than by oligonucleotides adsorbed into nanospheres.Conclusion ASODN in NP has good stability,encapsulation efficiency,drug loading and great potential for ASODN delivery.