ABSTRACT
Acupuncture regulating gastrointestinal motility has the characteristics of bidirectional benign regulation, acupoint specificity and immediacy. And its regulation is mainly achieved through the "neuro-endocrine-immune" network system. Acupuncture at Neiguan (PC 6) and Hegu (LI 4) to inhibit intestinal peristalsis may have good application value in colonoscopy.
Subject(s)
Acupuncture Points , Acupuncture Therapy , Colonoscopy , Gastrointestinal Motility , PeristalsisABSTRACT
O cogumelo tibetano (CT) é uma bebida probiótica fermentada por um grande conjunto de bactérias e leveduras, algumas das quais de conhecida atividade antimicrobiana, imunomodulatória, pró-digestiva e auxiliar da peristalse intestinal. Neste sentido, este trabalho teve como objetivo avaliar a possível ação da suspensão produzida pelo CT sobre a motilidade intestinal de ratos. Foram utilizados grupos de animais (ratos Wistar albinos machos, n = 6/grupo) pesando 200 ± 20 g. Os diferentes grupos foram tratados v.o. durante 15 dias (1,5 mL/animal de fermentado de 24 h de CT em cultivo contínuo em 50 g/l de açúcar mascavo comercial em água destilada), e os demais grupos tratados com água destilada (1,5 mL/animal). Após 15 dias os animais foram mantidos em jejum por 24 hs, e tratados com suspensão de CT (1,5 mL/animal/v.o), água destilada (controle, 1,5mL/animal/v.o), acetilcolina (1mg/kg/s.c.) e atropina (1mg/kg/i.m.). Após 30 min, os animais receberam uma suspensão de carvão ativo 10 por cento (0,1 mL/10 g, v.o), seguindo-se eutanásia após 45 min, excisão da porção do piloro até o início do ceco, e determinação da distância percorrida pelo carvão. A administração da suspensão de CT aumentou o trânsito intestinal em 68,7 por cento e os demais tratamentos apresentaram as seguintes percentagens: atropina 20,2 por cento, acetilcolina 78,7 por cento e controle 42,9 por cento (p < 0,05, ANOVA seguido de teste de Tukey-Kramer). Esses resultados mostraram que a suspensão de CT estimulou o peristaltismo nos animais, o que sugere um efeito positivo na digestão e absorção alimentar para o consórcio microbiano.
The Tibetan mushroom (CT) is a probiotic beverage fermented by a big group of bacteria and yeasts, some of which of well-known anti-bacterial activity, immunomodulatory, pro-digestive and to aid of the intestinal peristalsis. In this sense, this work had the aim of evaluating the possible action of the suspension produced by CT on the peristalsis intestinal of rats. Animal groups weighing 200 ± 20 g were used (Wistar rats (male, n = 6/group)). The different groups were treated v.o. for 15 days (1,5 mL/animal of 24h fermented of CT in continuously cultured into molasses solutions (50 g/l)), and the other groups treated with distilled water (1,5 mL/animal). After 15 days the animals were maintained in fast for 24h, and treated with suspension CT (1,5 mL/animal/v.o), distilled water (control, 1,5mL/animal/v.o), acetylcholine (1mg/kg/s.c) and atropine (1mg/kg/i.m). After 30 min, the animals received a suspension of active coal 10 percent (0,1 mL/10g, v.o), after the euthanasia, excision of the portion of the pylorus to the beginning of the cecum, and determination of the distance traveled by the coal. The administration of the CT suspension increased the intestinal peristalsis in 68,7 percent and the other treatments presented the following percentages: atropine 20,2 percent, acetylcholine 78,7 percent and control 42,9 percent (p < 0,05, ANOVA followed by Tukey-Kramer test). Those results showed that the CT suspension stimulated the peristalsis in the animals, what suggests a positive effect in the digestion and alimentary absorption for the microbial consortium.
ABSTRACT
The purpose of this study was to verify whether small intestinal peristalsis could be observed and quantitatively assessed using pulsed-Doppler ultrasound. Pulsed-Doppler ultrasound was used to evaluate small intestinal peristalsis after a meal in ten normal dogs and ten sedated dogs. The small intestinal peristalses were measured 0, 1, 3, 6, 9, 12, and 24 hours after a 24-hour fast and after feeding. The number of small intestinal peristalsis were 0.133/min, 0.100/min, 0.033/min, 0.167/min, 0.070/min, 0.067/min, and 0.100/min in the fasted dogs, and 1.667/ min, 0.933/min, 1.133/min, 1.234/min, 1.933/min, 1.533/ min, and 0.533/min in fed dogs, respectively. In the dogs sedated with xylazine HCl, the number of small intestinal peristalsis was significantly reduced (p<0.01). However, in the dogs treated with ketamine HCl and acepromazine, the number of small intestinal peristalsis remained unchanged. Therefore, it can be concluded that pulsed-Doppler ultrasound allows graphic visualization of the intestinal movements, which can be subjected to qualitative and quantitative analysis, and may be suitable for a non-invasive study of small intestinal motility.
Subject(s)
Animals , Dogs , Acepromazine/pharmacology , Dopamine Antagonists/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Gastrointestinal Motility/drug effects , Intestine, Small/drug effects , Ketamine/pharmacology , Peristalsis/drug effects , Ultrasonography, Doppler, Pulsed/methodsABSTRACT
Aim To study mechanisms of Stigma Maydis Polysaccharide(SMPS) on weight loss in experimental animals. Methods Observe changes of gastric discharge, intestinal peristalsis, biliary secretion, weight of gallbladder, body weight, appetite and cholecystokinin(CCK)level in plasm of rats and mice after being administrated with SMPS. Results SMPS increased the gastric residual rate of methyl orange and the intestinal propulsive rate,advanced first time black dejecta,increased the quantity of dejecta,decreased the weight of gallbladder in mice. SMPS also inhibited appetite,decreased body weight,prolonged gastric emptying,accelerated the propulsion speed of intestinal contents,promoted the biliary secretion,increased CCK level in plasm. Conclusion Mechanisms of weight loss are probably related to the increasing of CCK level in plasm and prolonging of gastric emptying,appetite inhibition, accelerating of intestinal peristalsis,the quantity increasing of dejecta.