ABSTRACT
OBJECTIVE: To establish a determination method of lignans in Shengmai injection. METHODS: On the basis of the study on the chemical constituents of Schisandrae Chinensis Fructus extracts, the peaks in the chromatograms of Shengmai injections from eight production enterprises were identified by HPLC-MS/MS. Agilent 6320 Ion Trap LC/MS, the separation was carried out by using an Waters symmetry shield TM RP18 column (4.6 cm × 25 cm, 5 μm), with a gradient mobile phase of acetonitrile-ammonium acetate water solution, detector wavelength was 203 nm; MS parameters; nebulizer: 2 kPa; dry gas: 12 mL · min-1; dry temperature 350°C; collision pressure: 1.0 V; positive ion scan. RESULTS: By comparing the Schisandrae Chinensis Fructus extracts and the Shengmai injection, it indicated that the lignans is the primary component in Schisandrae Chinensis Fructus extracts, and the mutual ingredients of the 8 enterprises is schizandrol A, gomisin D, gomisin J and angeloylgomisin Q. CONCLUSION: The established HPLC-MS/MS method can be used as the basis for further study and quality control of lignans in Shengmai injection.