ABSTRACT
Objective To study the anti-inflammatory constituents from the roots and rhizomes of Iris scariosa and I. bloudowii. Methods The chemical constituents were isolated and purified by column chromatography (silica gel, ODS, and sephadex LH-20), RP-preparative TLC, and semi-preparative HPLC. Their structures were identified by spectroscopic methods (MS, 1H-NMR, and 13C-NMR). The anti-inflammatory activities of the compounds were evaluated by the inhibition against NO production in lipopolysaeeharide (LPS) activated RAW 264.7 macrophages. Results Five compounds including tectoridin (1), iridin (2), nigricin 4’-O-β-D-glucoside (3), neomangiferin (4), and mangiferin (5) were isolated and identified from the roots and rhizomes of I. scariosa. Besides, seven compounds, nigricin4’-O-β-D-glucoside (3), nigricin (6), nigricanin (7), 5,5’-dihydroxy-3’,4’-dimethoxy-6,7- methylenedioxyisoflavone (8), nigricanin-4’-O-β-D-glucopyranoside (9), irifloside (10), and nigricin-4’-[O-β-D-glucopyranosyl- (1’’’→6″)-β-D-glucopyranoside] (11) were isolated and identified from the roots and rhizomes of I. bloudowii. Compounds 2, 3, 5, 6, 7, 9, 11, and previously reported chemical constituents of I. scariosa irigenin and irilone could inhibit NO production in RAW 264.7 macrophages to some extent. Conclusion Compounds 1-5 are isolated from I. scariosa and compounds 4 and 6-11 are isolated from I. bloudowii for the first time, respectively. The results of bioactivity assays indicated that compounds 2, 3, 5, 6, 7, 9, 11, and other two compounds irigenin and irilone isolated and purified from I. scariosa earlier are potential anti-inflammation agents.
ABSTRACT
Objective: To investigate the chemical constituents in the roots and rhizomes of Iris scariosa and Iris halophila var. sogdiana, respectively. Methods: The chemical constituents were isolated and purified by column chromatography (silica gel, ODS, Sephadex LH-20, and macroporous resin HP-20) and RP-preparative TLC. Their structures were identified by spectroscopic methods (MS, 1H-NMR, 13C-NMR, and 2D-NMR). Results: Six compounds, irigenin (1), nigricin-4'-[O-β-D-glucopyranosyl-(1‴→6″) - β-D-glucopyranoside] (2), 5, 7, 2'-trihydroxy-6-methoxy-flavanone (3), nigricin (4), irilone (5), and iriflophenone (6) were isolated and identified from the roots and rhizomes of I. scariosa. Besides, seven compounds, 5, 7, 2'-trihydroxy-6-methoxy-flavanone (3), irilin A (7), iristectorigenin B (8), iristectorigenin B 4'-O-β-D-glucopyranoside (9), iristectorigenin B 4'-[O-β-D-glucopyranosyl-(1‴→6″) - β-D-glucopyranoside (10), alpinone (11), and β-daucosterol (12) were isolated and identified from the roots and rhizomes of I. halophila var. sogdiana. Conclusion: Compound 9 is a new one named halophiloside. Compounds 1-6 are isolated from I. scariosa and compounds 3 and 7-12 are isolated from I. halophila var. sogdiana for the first time, respectively.