ABSTRACT
OBJECTIVE To establish the methods to identify the chemical components of Ixeris chinensis， and determine the contents of 7 components （chlorogenic acid， luteolin， quercetin， rutin， protocatechuic acid， isochlorogenic acid A， luteoloside）. METHODS HPLC-Q-Exactive-MS was used to identify the chemical components of I. chinensis. The contents of 7 components in I. chinensis， including chlorogenic acid， were determined by HPLC-MS/MS. RESULTS A total of 45 components were identified in I. chinensis， including 20 organic acids， 13 flavonoids， 4 fatty acids， 4 amino acids， 3 nucleosides， and 1 coumarin. The linear range of chlorogenic acid， luteolin， quercetin， rutin， protocatechuic acid， isochlorogenic acid A and luteoloside were 503.00- 25 150.00， 42.00-2 100.00， 5.05-252.50， 20.05-1 002.50， 25.10-1 255.00， 750.00-37 500.00， 196.00-9 800.00 ng/mL （r≥0.999 2）， respectively. RSDs of precision， stability and reproducibility tests were all less than 3.00% （n＝6）， and average recovery ranged from 96.72% to 105.84% （all RSD＜4.00%， n＝6）. The contents of 7 components in 3 batches of I. chinensis were 1 145.77- 3 261.25， 23.75-97.90， 0.92-2.12， 1.06-23.18， 9.35-21.85， 833.25-1 045.58， 199.56-1 869.78 μg/g， respectively. CONCLUSIONS The established methods for identification and content determination are rapid and simple， and can be used for the identification of chemical components and the content determination of 7 components in I. chinensis.
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OBJECTIVE： To screen the hepatoprotective active fractions from Ixeris chinensis and study its chemical constituents. METHODS：The petroleum ether，ethyl acetate，n-butanol and residual water fractions from 70％ ethanol extract of I.chinensis were extracted by systematic solvent method. Human hepatocytes HL-7702 were induced by acetaminophen to induce liver injury model. MTT method was used to detect the protective effect of the above fractions（40 μg/mL，by the dosage of crude drug）on injured cells，and the active fractions were screened. The active fractions were separated and purified by silica gel column and Sephadex column chromatography. The structure of the compounds were identified by physical and chemical properties and spectral data （hydrogen spectrum，carbon spectrum）. RESULTS：After treated with different fractions of I. chinensis，the cell survival rate of each administration group was increased significantly，compared with model group（P＜0.01），and the n-butanol and water fractions had the strongest activity （the cell survival rates were 49.3％ and 52.2％ ，respectively）. Six compoundswere isolated from n-butanol fraction and identified as sonchifolignan A（Ⅰ），apigenin-7-O-β-D-glucopyranoside methyl ester（Ⅱ），luteolin-7-O-β-D-glucuronopyranoside methyl ester （Ⅲ），luteolin-7-O-β-D-glucopyranoside （Ⅳ），apigenin-7-O-β-Dglucopyranoside（Ⅴ）and luteolin（Ⅵ）. CONCLUSIONS：The n-butanol fraction is regarded as an effective position for protecting liver，and flavonoids are the main active omponents.KEYWORDS Ixeris chinensis；Hepatoprotective activi
ABSTRACT
Objective: To identify the similarities and differences of the main chemical constituents between Ixeris chinensis and Sonchus brachyotus by UHPLC-Q exactive orbitrap-HRMS. Methods: The analysis was performed on an Agilent Poroshell 120 reverse phase column (100 mm × 3 mm, 2.7 μm). The mobile phase consisted of methanol and 0. 5% acetic acid, which was used for gradient elution, and the flow rate was 0.3 mL/min. Q exactive orbitrap-HRMS spectrometry was applied for the qualitative analysis under positive and negative ion modes, and ESI ion source was used for mass spectra. Results: The results indicated that nine compounds from the ethanol extract of I. chinensis and ten compounds from the ethanol extract of S. brachyotus had been identified by direct comparison in both positive and negative ion mass data, the element compositions analysis, and the data of the literature. Among them, there were nine compounds are the same, which were seven organic acids and two flavonoids compounds. Conclusion: The efficient separation ability of UHPLC and high sensitive detection of MS were used in this study, which will provide the evidences for evaluating the quality of I. chinensis and S. brachyotus and stabilizing the curative effect in clinic.
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Objective: To study the chemical constituents of Ixeris chinensis. Methods: The chemical constituents were isolated and purified by chromatography of silica gel column and HPLC, and their structures were elucidated by spectral analysis. Results: Nine triterpenoids were isolated and identified as 20α-peroxide-3β-uvaol-21-en (1), 3β,21α-dihydroxylupen-18-en (2), 3β,25-dihydroxy-tirucalla-7,23-diene (3), 21α-hydroxy-taraxasterol-20(30)-en (4), lupeol (5), 3β-uvaol (6), 3β-oleanol-18-en (7), 3β-oleanol (8), and 3β-uvaol-20-en (9). Conclusion: Compound 1 is a new compound, named ixeritriterpenol, and compound 7 is isolated from I. chinensis for the first time.
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OBJECTIVE: To study the chemical constituents of Ixeris chinensis Nakai. METHODS: The constituents were isolated by silica gel column chromatography, HPLC and recrystallization, and their structures were elucidated on the basis of spectral analysis. RESULTS: Fifteen compounds were isolated and identified as Chinensioide F(1), Chinensioide C(2), daucosterol(3), 6'-phydroxyphenylacetyl-Ixerin D(4), methyl-4-hydroxyphenylacetate(5), p-hydroxyphenylethanol(6), 3,5-dimethoxy-4-hedroxyphenylpropynol(7), 10α-hydroxy-guaia-12,6-lactone-3-keton(8), sitosterol(9), Chinensioide E(10), Chinensioide D(11), Ixerochinoside(12), 3β,10α-dihydroxy-guaia-4(15), 11(13)-diene-12,6-lactone(13), 10α-hydroxy-11βH-guaia-4(15)-ene-12,6-lacton(14) and luteolin-7-O-β-D-glucoside(15). CONCLUSION: Compound 1 is new (chinensiode F). Compound 4~7 and 12~14 were isolated from I. chinensis Nakai for the first time.