ABSTRACT
Objective: To compare the effects of Jiaoai Decoction and Siwu Decoction on peripheral hemogram, immune organ, and energy metabolism enzyme activity in blood deficiency model rats, and investigate the mechanism of Jiaoai Decoction derived as a tonic effect of Siwu Decoction after the change. Methods: Taking every day on the fundus venous plexus put blood 5 mL/kg, 12 d continuous bleeding, to establish the rat model of blood deficiency, the red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), platelet (PLT), immune organ index, and energy metabolism enzyme activity were detected. Results: Compared with the normal control group, RBC, HGB, HCT, and PLT could be significantly decreased (P2+-Mg2+-ATP and Na+-K+-ATP enzyme activity could be decreased significantly (P2+-Mg2+-ATP and Na+-K+-ATP enzyme activity could be enhanced (P2+-Mg2+-ATP enzyme activity could be enhanced (P+-K+-ATP enzyme activity was significantly weaker than that in the mid-dose group of Jiaoai Decoction. Conclusion: Jiaoai Decoction and Siwu Decoction have the effect of enriching the blood in different degrees, Jiaoai Decoction evolved into Siwu Decoction could decrease the efficacy of enriching the blood.
ABSTRACT
Objective: To screen the active parts with enriching the blood effects of different solvent extraction parts from Jiaoai Decoction (JAD) by comparing the peripheral blood, immune organ index, interleukin 2 (IL-2), and erythropoietin (EPO) and the effect of content of erythrocyte membrane and energy metabolism enzyme activity of blood deficiency model rats. Methods: Taking the fundus venous plexus blood 5 mL/kg every day, continuous bleeding for 12 d, to establish the rat model of blood deficiency; The model animals in each group were ig given JAD and n-butanol, petroleum ether, ethyl acetate, parts of water extract (12 g/kg crude drug) with compound E-Jiao slurry as a positive control. On day 12 of modeling, the erythrocyte count (RBC), hemoglobin (HGB), red blood cell hematocrit (HCT), platelet count (PLT) were detected in rats and immune organ indexes of spleen and thymus were calculated, the levels of IL-2 and EPO content were detected, energy metabolism enzymes (Na+, K+-ATPase and Ca2+, Mg2+-ATPase) activity were investigated; The difference of the chemical constituents in the different solvent extraction of JAD was analyzed by UPLC. Results: Compared with the control group, the RBC, HGB, HCT, PLT, thymus index, and IL-2 levels of rats in the model group decreased significantly, Na+, K+-ATPase and Ca2+, Mg2+-ATPase activity weakened significantly, the spleen index and EPO levels were significantly increased, indicating that blood deficiency model was successfully established. Compared with the model group, HCT, RBC, HGB, and PLT (P<0.05, 0.01) of rats in n-butyl alcohol group could be significantly increased; The spleen index and the content of EPO (P<0.05, 0.01) were reduced; The thymus index and the content of IL-2 (P<0.01) were increased, the erythrocyte Ca2+, Mg2+-ATPase and Na+, K+-ATPase activity (P<0.05, 0.01) was elevated. The contents of PLT and IL-2 (P<0.01) in petroleum ether group could be obviously increased; The content of EPO and the spleen index (P<0.05, 0.01) were decreased. The ethyl acetate fractions could obviously reduce the spleen index and the content of EPO (P<0.01), increase Ca2+, Mg2+-ATPase activity in erythrocytes (P<0.05). In water group PLT (P<0.05) could be significantly increased and the content of EPO (P<0.01) be decreased. There were no coffee acid, benzoyl paeoniflorin, and ammonium glycyrrhizinate in other solvent extracts of JAD, while the content of gallic acid, protocatechuic acid, ligustrazine phosphate, and chlorogenic acid in n-butanol extract were higher than those in ethyl acetate and water extract parts of JAD. Conclusion: The n-butanol extract is the most active part of JAD for enriching the blood.
ABSTRACT
Objective: To compare the effects of Jiaoai Decoction and Siwu Decoction on hemorheology, blood coagulation function, and platelet aggregation in acute blood stasis rats, and reveal that Jiaoai Decoction evolved into Siwu Decoction could increase the efficacy of activating blood circulation and dissipating blood stasis. Methods: Ice water bath and sc injection of adrenaline were both used to establish the acute blood stasis rat model. The whole blood viscosity (WBV), plasma viscosity (PV), haematocrit (HCT), thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), plasma fibrinogen (FIB), and platelet aggregation (PAG) were detected to observe the effects of Jiaoai Decoction and Siwu Decoction on the acute blood stasis rat model. Results: Compared with the normal control group, the model group could significantly increase hemorheological indices (P < 0.01), shorten TT, PT, and APTT (P < 0.01), and increase the FIB content (P < 0.01) and platelet aggregation (P < 0.01). Compared with the model group, Siwu Decoction could significantly decrease WBV, PV, and HCT (P < 0.05, 0.01), prolong TT, APTT, and PT (P < 0.05), and reduce the content of FIB (P < 0.01) and platelet aggregation (P < 0.01). The mid-dose Jiaoai Decoction could significantly decrease WBV in 1 s-1 and HCT (P < 0.05, 0.01), prolong APTT (P < 0.05) and shorten PT (P < 0.01), and reduce the platelet aggregation (P < 0.01). Compared with mid-dose Jiaoai Decoction, Siwu Decoction could significantly prolong the TT (P < 0.01). Conclusion: Jiaoai Decoction evolved into Siwu Decoction could increase the efficacy of activating blood circulation and dissipating blood stasis. Meanwhile, the study reveals the scientific evidence for Jiaoai Decoction and Siwu Decoction in clinical application.