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Objective To investigate the protective effect of Jiawei Danshen Yin combined with microRNA-21 on IRI myocardial cells,and to study its protective mechanism. Methods H9C2 cardiomyocytes were cultured and divided into four groups: Blank group, IRI model group of cardiomyocytes,miR-21 group,miR-21 combined with Jiawei Danshen Yin group (JWDSY group). Apoptotic rate, apoptosis-related protein and PTEN signaling pathway expression of cardiomyocytes were detected. Results Electron microscopy showed that mitochondria in model group were damaged significantly , and ultrastructural damage in JWDSY group was alleviated; ELISA demonstrated that cTnl was elevated in model group and decreased in JWDSY group with statistical significance (P < 0. 01); flow cytometry re vealed that the apoptotic rate was significantly increased in model group (36. 79 ±2. 12) ,but markedly decreased in JWDSY group (14. 65 ± 0. 94), and the difference was statistically significant ( P < 0. 05 ). Western blot results showed that PTEN expression was uP-regulated and P-Akt expression down-regulated in model group,while PTEN expression decreased and P-Akt expression increased in JWDSY group, and the difference was statistically significant ( P < 0. 05 ). Conclusions miR-21 combined with Jiawei Danshen Yin can inhibit cardiomyocyte apoptosis and protect IRI cardiomyocytes by reducing PTEN expression and activating PI3K/Akt signaling pathway,.
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Objective:To investigate the effects of Jiawei Danshen Yin on the cardiac function and serum inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the rats with chronic heart failure, and to elucidate the mechanism of Jiawei Danshen Yin in the treatment of chronic heart failure.Methods:The SD rats were randomly divided into blank control group, heart failure model group, captopril group, and Low, medium, and high doses of Jiawei Danshen Yin groups.The rat models of chronic heart failure were prepared by intraperitoneal injection of adriamycin solution for 4 weeks.The left ventricular ejection fraction (LVEF) of the rats was measured by echocardiography.The heart rate (HR) , cardiac output (CO) , systolic blood pressure (SBP) , carotid artery diastolic pressure (DBP) , left ventricular end diastolic pressure (LVEDP) , left ventricular systolic pressure (LVSP) , left ventricular pressure change maximum increase/decrease rate (±dp/dtmax) and other related hemodynamic parameters were measured by ventilator in each group to assess the rat heart function.The levels of inflammatory cytokines IL-6and TNF-αwere detected by enzyme-linked immunosorbent assay (ELISA) .Results:Compared with blank control group, the LVEF of the rats in model group, captopril groups and low, medium, and high doses of Jiawei Danshen Yin groups were decreased (P＜0.05) .Compared with model group, the LVEF of the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were increased (P＜0.05) .The HR, SBP, DBP, CO, LVSP, and±dp/dtmaxof the rats in model group were lower than those in blank control group (P＜0.05) , while the LVEDP was increased (P＜0.05) .Compared with model group, the SBP, DBP, CO, LVSP and±dp/dtmax of the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were increased (P＜0.05) , however the HR and LVEDP were decreased (P＜0.05) .Compared with low dose of Jiawei Danshen Yin group, the CO and±dp/dtmaxof the rats in captopril group and medium and high doses of Jiawei Danshen Yin groups were increased (P＜0.05) and the LVEDP were decreased (P＜0.05) .Compared with blank control group, the levels of serum IL-6and TNF-αof the rats in the other groups were increased (P＜0.05) .The levels of serum IL-6and TNF-αof the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were lower than those in model group (P＜0.05) .Compared with low dose of Jiawei Danshen Yin group, the levels of serum IL-6and TNF-αof the rats in captopril group and medium and high doses of Jiawei Danshen Yin groups were decreased (P＜0.05) .Compared with blank control group, the indexes of heart and lung in the other groups were all increased (P＜0.05) ;compared with model group, the heart and lung indexes of the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were decreased (P＜0.05) .Conclusion:Jiawei Danshen Yin can improve the heart function of the rats with heart failure and inhibit the expressions of serum inflammatory cytokines IL-6and TNF-αto some extent.In the common dose range, Jiawei Danshen Yin can maximize the benefit of cardiac function and the serum inflammatory factors in the rats with chronic heart failure.
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Objective To study the effect of Jiawei-Danshen Yin on anti-myocardial fibrosis and Notch signaling pathway in rats with myocardial fibrosis. Methods The rats were randomly divided into normal group (10 rats), model group (5 rats), latopril group (5 rats), low dose group (5 rats) and high dose group (5 rats). In addition to the normal group, the other groups of rats prepared myocardial fibrosis model. One hour after model establishment, the rats in high and low dosage groups were given 1.5 mg/ml and 3.0 mg/ml of modified salvia miltiorrhiza drink respectively, the rats in captopril group were given 1 mg/ml of captopril, the rats in model group and normal group were given saline of equal volume for 1 day/time for one week. The collagen volume fraction (CVF) was measured by pathological observation. The left ventricular mass index (LVMI), heart mass index (HWI), heart rate (HR), left ventricular systolic pre-existing (LVSP) left ventricular end diastolic pressure (LVEDP) and left ventricular systolic pressure were measured. Western blot was used to detect the expression of Notch1, NICD, Notch3 and α-SMA. Results Compared with the model group, the levels of LVMI (2.26 ± 0.01 mg/g, 1.48 ± 0.03 mg/g vs. 2.86 ± 0.06 mg/g), CVF (3.44% ± 1.45%, 2.62% ± 1.43% vs. 7.82% ± 1.95%), HWI (4.05 ± 0.02 mg/g, 3.11 ± 0.02 mg/g vs. 4.58 ± 1.05 mg/g) in the low and high dose groups of Jiawei-Danshen Yin significantly decreased respectively(P<0.05). The levels of LVEDP (10.16 ± 2.35 kPa, 6.25 ± 1.23 kPa vs. 13.15 ± 3.06 kPa) significantly decreased, HR (375.22 ± 20.36 time/min, 450.32 ± 22.13 time/min vs. 329.36 ± 22.01 time/min), LVSP (90.12 ± 6.32 kPa, 110.45 ± 10.71 kPa vs. 80.54 ± 10.35 kPa) significantly increased (P<0.05) respectively. The expression of Notch1 (0.83 ± 0.05, 1.11 ± 0.04 vs. 0.51 ± 0.06), NICD (0.72 ± 0.01, 1.06 ± 0.03 vs. 0.35 ± 0.05), Notch3 (0.62 ± 0.03, 1.20 ± 0.06 vs. 0.39 ± 0.05) in myocardial tissue significantly increased, and the expression of α-SMA (1.12 ± 0.08, 0.92 ± 0.05 vs. 1.43 ± 0.07) significantly decreased (P<0.05). Conclusions The Jiawei-Danshen Yin has a significant effect on myocardial fibrosis model rats. It can improve the anti-myocardial fibrosis ability by activating Notch signaling pathway.
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Objective: To investigate the effects of Jiawei Danshen Yin on the cardiac function and serum inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-α) in the rats with chronic heart failure, and to elucidate the mechanism of Jiawei Danshen Yin in the treatment of chronic heart failure. Methods: The SD rats were randomly divided into blank control group, heart failure model group, captopril group, and Low medium, and high doses of Jiawei Danshen Yin groups. The rat models of chronic heart failure were prepared by intraperitoneal injection of adriamycin solution for 4 weeks. The left ventricular ejection fraction (LVEF) of the rats was measured by echocardiography. The heart rate (HR), cardiac output (CO), systolic blood pressure (SBP), carotid artery diastolic pressure (DBF), left ventricular end diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP) left ventricular pressure change maximum increase/decrease rate (±dp/dtmax) and other related hemodynamic parameters were measured by ventilator in each group to assess the rat heart function. The levels of inflammatory cytokines IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with blank control group, the LVEF of the rats in model group, captopril groups and low, medium, and high doses of Jiawei Danshen Yin groups were decreased (P<0. 05). Compared with model group, the LVEF of the rats in captopril group and low, medium, and high doses of Jiawei Danshen Yin groups were increased (P<0. 05). The HR, SBF, DBF, CO, LVSPt and ±dp/dtmax of the rats in model group were lower than those in blank control group (P<0. 05), while the LVEDP was increased ( P
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Objective@#To study the effect of Jiawei-Danshen Yin on anti-myocardial fibrosis and Notch signaling pathway in rats with myocardial fibrosis.@*Methods@#The rats were randomly divided into normal group (10 rats), model group (5 rats), latopril group (5 rats), low dose group (5 rats) and high dose group (5 rats). In addition to the normal group, the other groups of rats prepared myocardial fibrosis model. One hour after model establishment, the rats in high and low dosage groups were given 1.5 mg/ml and 3.0 mg/ml of modified salvia miltiorrhiza drink respectively, the rats in captopril group were given 1 mg/ml of captopril, the rats in model group and normal group were given saline of equal volume for 1 day/time for one week. The collagen volume fraction (CVF) was measured by pathological observation. The left ventricular mass index (LVMI), heart mass index (HWI), heart rate (HR), left ventricular systolic pre-existing (LVSP) left ventricular end diastolic pressure (LVEDP) and left ventricular systolic pressure were measured. Western blot was used to detect the expression of Notch1, NICD, Notch3 and α-SMA.@*Results@#Compared with the model group, the levels of LVMI (2.26 ± 0.01 mg/g, 1.48 ± 0.03 mg/g vs. 2.86 ± 0.06 mg/g), CVF (3.44% ± 1.45%, 2.62% ± 1.43% vs. 7.82% ± 1.95%), HWI (4.05 ± 0.02 mg/g, 3.11 ± 0.02 mg/g vs. 4.58 ± 1.05 mg/g) in the low and high dose groups of Jiawei-Danshen Yin significantly decreased respectively(P<0.05). The levels of LVEDP (10.16 ± 2.35 kPa, 6.25 ± 1.23 kPa vs. 13.15 ± 3.06 kPa) significantly decreased, HR (375.22 ± 20.36 time/min, 450.32 ± 22.13 time/min vs. 329.36 ± 22.01 time/min), LVSP (90.12 ± 6.32 kPa, 110.45 ± 10.71 kPa vs. 80.54 ± 10.35 kPa) significantly increased (P<0.05) respectively. The expression of Notch1 (0.83 ± 0.05, 1.11 ± 0.04 vs. 0.51 ± 0.06), NICD (0.72 ± 0.01, 1.06 ± 0.03 vs. 0.35 ± 0.05), Notch3 (0.62 ± 0.03, 1.20 ± 0.06 vs. 0.39 ± 0.05) in myocardial tissue significantly increased, and the expression of α-SMA (1.12 ± 0.08, 0.92 ± 0.05 vs. 1.43 ± 0.07) significantly decreased (P<0.05).@*Conclusions@#The Jiawei-Danshen Yin has a significant effect on myocardial fibrosis model rats. It can improve the anti-myocardial fibrosis ability by activating Notch signaling pathway.