ABSTRACT
Objective To develop a HPLC method for quantitative determination of orientin and vitexin in Jinlianhua Pills and to provide valuable data for quality control of Jinlianhua Pills.Methods The separation was performed on a Shimadzu VP-ODS column (250 x 4.6mm,5μm)at 35℃ using a mobile phase of 0.5% acetic acid solution-acetonitrile(88:12).The detection wavelength was 340nm.Results The calibration curve of orientin showed a good linearly within test ranges of 25.0 ~ 75.0μg/ml.The average recovery was 99.9% with RSD 1.2%.The calibration curve of vitexin showed a good linearly within test ranges of 8.0 ~ 24.0μg/ml.The average recovery was 100.5% with RSD 1.3%.Conclusion The developed method is simple,accurate and with good repeatability.It is suitable for quality evaluation of Jinlianhua Pills.